30,808 results

1. pJM251 (miniCTX1-araC-ParaBAD)

   Plasmid

   #110567

   Purpose

   Integration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoter

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   araC-ParaBAD
   

   Expression

   Bacterial

   Promoter

   araC-ParaBAD

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBADHISTEV_PfeE

   Plasmid

   #129120

   Purpose

   pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   PBAD

   Available Since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJKR-L-tetR

   Plasmid

   #62562

   Purpose

   Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNBU2_erm_P1T_DP-GH023

   Plasmid

   #90317

   Purpose

   Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS GH023

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHH0103_ESRRA_Hormone-recep

   Plasmid

   #109908

   Purpose

   Protein expression and purification of ESRRA_Hormone-recep

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   ESRRA_Hormone-recep (ESRRA Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-pro-sumo1

   Plasmid

   #25101

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Changed Serine 2 to Alanine

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pBAD-eLACCO2.1

   Plasmid

   #208017

   Purpose

   Biosensor for extracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   eLACCO2.1
   

   Expression

   Bacterial

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFGM1

   Plasmid

   #64949

   Purpose

   source of GmR FRT cassette

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   FRT-GmR-FRT
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEN017-SpCas9-hiNLS-t-M4

   Plasmid

   #234455

   Purpose

   Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGGG-Sr43

    Plasmid

    #186974

    Purpose

    Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).

    Depositor

    Brande Wulff

    Article

    Yu et al Nat Genet. 2023 Jun;55(6):921-926. doi:

    Insert

    Sr43
    

    Tags

    None

    Expression

    Bacterial and Plant

    Mutation

    None

    Promoter

    Natice

    Available Since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJUMP45-2A(sfGFP)

    Plasmid

    #126989

    Purpose

    Level 2 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174840

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    ENO1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZE31ms2

    Plasmid

    #25860

    Depositor

    Philippe Cluzel

    Article

    Le et al Proc Natl Acad Sci U S A. 2005 Jun 28. 1

    Insert

    ms2 binding site (x2)
    

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pExchange_tdk_BT1311p-tetR-2

    Plasmid

    #90326

    Purpose

    Suicide vector for integration of a constitutively expressed TetR by promoter BT1311 at B. thetatiotaomicron genomic position 2660693 and 2660694

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    TetR
    

    Expression

    Bacterial

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-TAG

    Plasmid

    #26771

    Depositor

    Shankar Srinivas

    Article

    Trichas et al BMC Biol. 2008 Sep 15;6:40. doi: 10.1186

    Insert

    CAG-TdTomato-2A-H2BGFP-SV40pA
    

    Expression

    Bacterial

    Available Since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDsEndoNA2

    Plasmid

    #174728

    Purpose

    Expresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Lehti et al Nat Commun. 2017 Dec 4;8(1):1915. doi: 1

    Insert

    DsRed-Monomer
    

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ibpAB-GFP

    Plasmid

    #109390

    Purpose

    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pibpAB-GFP
    

    Expression

    Bacterial

    Available Since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gi-c010

    Plasmid

    #173080

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GAP
    

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    mCitrine + last 10 residues of GNA1, codon optimi…

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-30 Ubwt (2)

    Plasmid

    #26685

    Depositor

    Linda Hicke

    Article

    French et al J Biol Chem. 2009 May 1. 284(18):12071-9

    Insert

    Ubiquitin
    

    Tags

    His and S-tag

    Expression

    Bacterial

    Available Since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only