30,320 results

1. pJMP2834

   Plasmid

   #160673

   Purpose

   Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Insert

   gmc6 (gfp) sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. His-TEV-Aurein1.2_(+36GFP)-LPETG-His

   Plasmid

   #71755

   Purpose

   His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   Aurein1.2_(pos36_GFP)-LPETG-His
   

   Use

   Tags

   6XHis, Sortase (LPETG), and TEV Protease

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_PABPC1_PABP

   Plasmid

   #109931

   Purpose

   Protein expression and purification of PABPC1_PABP

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PABPC1_PABP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PERK 5: PERK.K618R.pGEX4T-1

   Plasmid

   #21818

   Depositor

   David Ron

   Article

   Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

   Insert

   PERK (537-1114) K618R (Eif2ak3 Mouse)
   

   Use

   Tags

   GST and Myc (9E10)

   Expression

   Bacterial

   Mutation

   PERK Kinase Domain. Amino acids 537-1114. Kinase …

   Promoter

   Available since

   March 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOSV805

   Plasmid

   #126598

   Purpose

   modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

   Depositor

   Jean-Luc Pernodet

   Article

   Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYDE007

   Plasmid

   #194152

   Purpose

   sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   sgRNA with mrfp control guide sequence
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

   Plasmid

   #85072

   Purpose

   Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor constitutively active (Q63L) (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q63L

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

   Plasmid

   #105000

   Purpose

   Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TetCMV

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T-HEPN

   Plasmid

   #75296

   Purpose

   Expresses Sacsin HEPN domain (4441-4579) in fusion with GST (bacterial)

   Depositor

   Kalle Gehring

   Article

   Kozlov et al J Biol Chem. 2011 Jun 10;286(23):20407-1

   Insert

   Human Sacsin 4441-4579
   

   Use

   Tags

   GST-Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PE-SUMO CRYAB

    Plasmid

    #217670

    Purpose

    For expression of SUMO tagged human alpha B crystallin

    Depositor

    Larry David

    Insert

    CRYAB (CRYAB Human)
    

    Use

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFEndoNA

    Plasmid

    #174724

    Purpose

    Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA fusion protein
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCD565

    Plasmid

    #153027

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A), J306 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-OptoPB

    Plasmid

    #113935

    Purpose

    Express a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactions

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    OptoPB
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

    Plasmid

    #166421

    Purpose

    Express pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Naegleria fowleri PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6xHis(TEV)-Drp1(K38A)-StrepII

    Plasmid

    #174423

    Purpose

    Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K38A

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pScaf-5544.4

    Plasmid

    #111524

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v4)

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-KH'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)

    Plasmid

    #136543

    Purpose

    Expresses ANXA2 chimeras in bacterial cells

    Depositor

    Laszlo Nyitray

    Article

    Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

    Type

    Empty backbone

    Use

    Tags

    ANXA2 (23-339) and His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSCC2

    Plasmid

    #39993

    Depositor

    Paul Modrich

    Article

    Cheng et al J Biol Chem. 1984 Sep 25;259(18):11571-5

    Insert

    EcoRI endonuclease & EcoRI methylase
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    n/a

    Available since

    Nov. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-Tev-WWP2

    Plasmid

    #186807

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-WWP2 (WWP2 Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. EcMscL

    Plasmid

    #92418

    Purpose

    E. coli Mechanosensitive Channel of Large Conductance

    Depositor

    Douglas Rees

    Insert

    MscL (mscL E. coli)
    

    Use

    Tags

    6x Histidine Tag and Thrombin Cleavage Site betwe…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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