30,808 results

1. K980

   Plasmid

   #129762

   Purpose

   Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAMK2D

   Plasmid

   #38987

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CAMK2D (CAMK2D Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pET21a-GFP-AURKA_K162M-mCherry

   Plasmid

   #99881

   Purpose

   Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP and mCherry

   Expression

   Bacterial

   Mutation

   K162M (kinase-dead)

   Available Since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRF-McbR

   Plasmid

   #49365

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   McbR repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG28-J23151-AraC

   Plasmid

   #102453

   Purpose

   Expresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   AraC (araC )
   

   Expression

   Bacterial

   Promoter

   J23151

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. TAF1L

   Plasmid

   #39014

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TAF1L (TAF1L Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pMRE161

   Plasmid

   #118509

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Expression

   Bacterial

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDule-Mb haloTyrRS C6

   Plasmid

   #160377

   Purpose

   C6 HaloTyrosine tRNA synthatase and cognate amber suppressing tRNA derived from M. barkeri.

   Depositor

   Ryan Mehl

   Article

   Jang et al ACS Chem Biol. 2020 Feb 21;15(2):562-574

   Insert

   C6 HaloTyrosine tRNA synthatase
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Mammalian

   Mutation

   L270S, Y271L, N311G, C313T

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pstb-LAFR5

   Plasmid

   #86169

   Purpose

   pLAFR5 with Smb21651 promoter. pLAFR5 is a broad-host range cosmid vector with double cos sites.

   Depositor

   Kay Jones

   Article

   Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   SMb21651 promoter

   Available Since

   Feb. 6, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX3X-RanBP2dFG

    Plasmid

    #53120

    Purpose

    E.coli expression of GST tagged human RanBP2 fragment with SUMO ligase activity

    Depositor

    Frauke Melchior

    Article

    Pichler et al Cell. 2002 Jan 11;108(1):109-20.

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2553-2838)

    Promoter

    tac

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSIBR003

    Plasmid

    #177666

    Purpose

    SIBR Intron 3 (Int3) interrupts the CDS of FnCas12a

    Depositor

    John van der Oost

    Article

    Patinios et al Nucleic Acids Res. 2021 Oct 6. pii: 6382

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQTEV-SCP2

    Plasmid

    #31336

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    sterol carrier protein 2 (SCP2 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-T4uvsW

    Plasmid

    #228201

    Purpose

    Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

    Depositor

    Stephen Benkovic

    Article

    Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

    Insert

    uvsW
    

    Tags

    hexahistidine and 10 amino acid linker

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pShHELIX(Cas12k-TniQ-TniQ)-sgRNA_entry (CJT112)

    Plasmid

    #181791

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TniQ-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ-ShTniQ
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCPP5060

    Plasmid

    #128716

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

    Insert

    shcN-hopN1
    

    Expression

    Bacterial

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCPP6450

    Plasmid

    #128731

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

    Insert

    HopAD1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Nsp3 Mac1 - P43 (Oxford)

    Plasmid

    #169210

    Purpose

    Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

    Depositor

    James S Fraser

    Article

    Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

    Insert

    SARS-CoV-2 Nsp3 Mac1
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. AMPKA2

    Plasmid

    #36194

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    AMPKA2 (PRKAA2 Human)
    

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    contains KINASE DOMAIN only

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pH6HTC_RBFOX2-Halo

    Plasmid

    #175304

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    RBFOX2 (RBFOX2 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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