31,591 results
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Plasmid#107876PurposeExpresses E. coli RtcA in bacterial expression cellsDepositorAvailable SinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only
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pUB-MSP1D1
Plasmid#161609PurposeBacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
Tags7-His tagExpressionBacterialMutationmutant of MSP1D1 ( Q138W, L181W, Y236W)PromoterT7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD-MBP-His-ACPs
Plasmid#141140PurposeExpression of holo-[acyl-carrier-protein] synthaseDepositorInsertacpS
TagsMBPExpressionBacterialAvailable SinceMay 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMEG_YLCas9_empty
Plasmid#117826PurposeCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolyticaDepositorInsertYlpTEF_hCas9_YlCyc1TT
ExpressionBacterial and YeastAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
TagsFLAG purification tag and eGFPExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTHmut
Plasmid#200159PurposeFor bacterial expression of the APOBEC1-YTHmut fusion protein for protein purificationDepositorInsertAPOBEC1-YTHmut
Tags6xHis, HA, and MBPExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
PHB1
Plasmid#212153PurposeFor PHB production in E. coli (PT7-pMB1).DepositorInsertpoly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
ExpressionBacterialPromoterPT7Available SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV303
Plasmid#204978PurposeAMA1 plasmid with Aspergillus optimized Mad7 and amdS selection markerDepositorInsertsMad7
amdS
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationtwo silent mutations (nt177T>C, nt879C>T)PromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor_Tn-pOUT
Plasmid#213905PurposeMariner transposon with outward-facing pJ23104 promoterDepositorInsertmariner transposon with kan resistance and outward-facine pJ23104 promoter
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-wt
Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE-meGFP-DLC1
Plasmid#192048PurposePlasmid expresses DLC1 binding peptide to Talin R8 domain in chimera with meGFPDepositorInsertDLC1 (DLC1 Human)
ExpressionBacterialAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-ATP
Plasmid#225247PurposeBacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GFP-Rab27a
Plasmid#194985Purposeoverexpress Rab27aDepositorInsertGFP-Rab27a
ExpressionBacterialAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pELlR4
Plasmid#172545PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Carbenicillin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2H
Plasmid#15752DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pTrcHUM
Plasmid#19003DepositorInsertgamma-humulene synthase
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG-RGG
Plasmid#124938PurposeExpression of RGG-RGG-RGGDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AlkB
Plasmid#228218PurposeExpress wild type AlkB protein in E. coli BL21(DE3)DepositorInsertAlpha-ketoglutarate-dependent Dioxygenase (alkB Synthetic)
TagsHis tagExpressionBacterialMutationcodon optimizedPromoterT7Available SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
RhoA
Plasmid#55699Purposeexpression of mouse RhoADepositorAvailable SinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
KLHL2
Plasmid#39060PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only