32,838 results
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Plasmid#154126PurposeExpresses wt rRNAs operon in bacterial cellsDepositorInsertwt bacterial rRNAs operon under control of pL promotor
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
SNX11-PX (7-167)
Plasmid#119092PurposeBacterial expression of human phox homology (PX) domain, SNX11-PX (7-167)DepositorAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHelper-T7IS1
Plasmid#140631PurposeExpresses ShCAST under the T7 promoter and an empty sgRNADepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
UseCRISPR; TransposonExpressionBacterialAvailable SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1-54 pComb3xss anti-cFos nanobody
Plasmid#145836PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody S1-54 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
GC92 pComb3xss anti-Gephyrin nanobody
Plasmid#145826PurposeBacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Gephyrin (Human) recombinant llama nanobody GC92 (GPHN L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSETT4gap
Plasmid#153413PurposeφC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassetteDepositorTypeEmpty backboneExpressionBacterialPromotergapDH promoter from Eggerthella lentaAvailable SinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJ2077.2
Plasmid#140666PurposeController plasmid for TS circuit and cL and cR controlsDepositorInsertController plasmid for TS circuit and cL and cR controls
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
LARS_pNIC-Bio3
Plasmid#153052PurposeExpression construct for producing Avi tagged Leucyl-tRNA synthetaseDepositorInsertLARS (LARS1 Human)
TagsAvi and His6-TEVExpressionBacterialMutationNot full length proteinPromoterT7Available SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
HARS_pNIC-Bio3
Plasmid#153050PurposeExpression construct for producing Avi tagged histidyl tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
CyPet-A206K-PRL2
Plasmid#149685PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCyPet-A206K-PRL2
Tags8 histidine tagExpressionBacterialMutationCyPet A206KPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AlphaC
Plasmid#124525PurposepColE1_AlphaCDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_target_42_mut_G
Plasmid#139467PurposeT7 RNAP-driven expression of WT trigger RNA with mutation at 42DepositorInserttarget_42_mut_G
ExpressionBacterialAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFloat.T7_v1
Plasmid#133070PurposeEntry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseGateway CloningTags3C-His6 and His6-3CExpressionBacterialPromoterT7Available SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM09
Plasmid#135482PurposeAgrobacterium T-DNA with SpunHsp70pr driving hph-GFPDepositorInsertSpunHsp70pr hph-GFP ADH1t
TagsGFPExpressionBacterial and YeastPromoterSpunHsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
NdeI – SacII – SpeI(NheI) - ANXA2 (23-339)
Plasmid#136543PurposeExpresses ANXA2 chimeras in bacterial cellsDepositorTypeEmpty backboneTagsANXA2 (23-339) and His-tagExpressionBacterialAvailable SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)
Plasmid#136546PurposeExpresses ANXA2 chimeras in bacterial cellsDepositorTypeEmpty backboneTagsANXA2 (23-339) and His-tagExpressionBacterialPromoterT7Available SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4095420
Plasmid#149523PurposeBacterial expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationd1-745 d1063-1945PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4136454
Plasmid#149529PurposeBacterial expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 13, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTNT-pT7-tetR-mCherry
Plasmid#140870PurposeT7 promoter driven synthesis of TetR-mCherry fusion proteinDepositorInsertTetR-mCherry expressed with T7 promoter
UseSynthetic BiologyTagsmCherryExpressionBacterialPromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c028
Plasmid#139765PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
TagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c027
Plasmid#139764PurposeProtein expression/Streptavidin pull-downDepositorInsertTBXT, Full-length, G177D (TBXT Human)
TagsAvi-tag (Biotin) and His6-GST-TEVExpressionBacterialMutationG177D; contains only amino acids S2- M435PromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c026
Plasmid#139763PurposeProtein expression/GST pull-downDepositorInsertTBXT, Full-length, WT (TBXT Human)
TagsHis6-GST-TEVExpressionBacterialMutationContains only amino acids S2- M435PromoterT7Available SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
TBXTA-c006
Plasmid#139755PurposeFor protein expression/crystallography (PDB:6F59)DepositorInsertTBXT, DNA-binding domain, G177D (TBXT Human)
TagsHis6ExpressionBacterialMutationG177D; contains only amino acids E41-S224PromoterT7Available SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-gfp
Plasmid#128141PurposeDinoflagelate backbone for gfp expressionDepositorInsertGreen fluorescence protein
ExpressionBacterialPromoterDinoflagellate potential promotersAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
DinoIII-arrO-N
Plasmid#128143PurposeDinoflagellate backbone with rifampicin resistant gene optimized for Oxyrrhis marinaDepositorInsertRifampin ADP-ribosylating transferase + potential promoter
ExpressionBacterialAvailable SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046953
Plasmid#145764PurposeBacterial Expression plasmid for SARS-CoV-2 ORF7bDepositorInsertSARS-CoV-2 ORF7b (ORF7b Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046801
Plasmid#145741PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;MycExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046890
Plasmid#145740PurposeBacterial Expression plasmid for SARS-CoV-2 ORF6 proteinDepositorInsertSARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046925
Plasmid#145694PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046919
Plasmid#145615PurposeBacterial Expression plasmid for SARS-CoV-2 nsp7DepositorInsertSARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits