34,237 results

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pET22b-T5-sfGFP*- MmH-MH4R

Plasmid

#196409

Purpose

Express sfGFP with TAG and enzymes for biosynthesizing DOPA

Depositor

Han Xiao

Publication

Chen et al J Mol Biol. 2021 Dec 20:167412. doi: 10.

Inserts

sfGFP with TAG at 151 position
Tyrosine Hydroxylase (Th Mouse)
dihydropterine reductase from human (QDPR Human)
pterin-4a-carbinolamine dehydratase from human

Expression

Bacterial

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ-mNeongreen

Plasmid

#182392

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-LZ-mNeongreen

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMV361_MjTyrRS

Plasmid

#193250

Purpose

Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

Depositor

Peter Schultz, Jessica Seeliger

Publication

Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

Insert

MjTyrRS

Expression

Bacterial

Promoter

hsp60

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSMT-MjtRNA-GFP(47TAG)

Plasmid

#193251

Purpose

Expresses the M. jannaschii tRNATyr (MjtRNA) and GFP(47TAG) (aka GFP151TAG)

Depositor

Peter Schultz, Jessica Seeliger

Publication

Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

Inserts

GFP(47TAG)
MjtRNA

Expression

Bacterial

Mutation

Codon for Tyr47 replaced with amber stop codon TA…

Promoter

hsp60 and metU

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMG024 MBP-GSK3β-HA-His, GST_λPPase

Plasmid

#196182

Purpose

Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His

Depositor

Jesse Zalatan

Publication

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Inserts

GSK3B (GSK3B Human)
λPPase

Tags

GST, HA, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_INS

Plasmid

#184867

Purpose

pSwap plasmid part with insert fragment module

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSWAP_Entry_HB

Plasmid

#184868

Purpose

pSwap plasmid part with HB homology arms

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDropVn

Plasmid

#184873

Purpose

Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for Vibrio natriegens genome editing protocol.

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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cr3Vn

Plasmid

#184861

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an V. natriegens-specific recombination system

Depositor

Patrick Sobetzko

Publication

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, gam, bet, exo

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-mLemon

Plasmid

#191256

Purpose

Bacterial expression plasmids for pH sensitive fluorescent protein mLemon

Depositor

Robert Campbell

Insert

mLemon

Tags

His tag

Expression

Bacterial

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-ATAD2-plx303

Plasmid

#188635

Purpose

ATAD2 overexpression

Depositor

Lorenz Studer

Publication

Baggiolini et al Science. 2021 Sep 3;373(6559):eabc1048.

Insert

ATAD2 (ATAD2 Human)

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-FOLD6

Plasmid

#186547

Purpose

FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

FOLD6

Tags

6xHis

Expression

Bacterial

Mutation

mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

Promoter

araB

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-ALDH1A3

Plasmid

#189750

Purpose

The construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.

Depositor

Robert Sobol

Publication

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A3 (ALDH1A3 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-mWatermelon

Plasmid

#190625

Purpose

Bacterial expression of mWatermelon GFP

Depositor

Robert Campbell

Publication

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

mWatermelon

Expression

Bacterial

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.402

Plasmid

#187202

Purpose

Expresses retron Eco1 RT

Depositor

Seth Shipman

Publication

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Insert

Retron Eco1 Reverse Transcriptase

Use

Synthetic Biology

Expression

Bacterial

Promoter

mphR

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGE144 (MutaEco; aTC-induced)

Plasmid

#189824

Purpose

Expresses MutaEco for generating mutations in the E. coli genome (aTC-induced)

Depositor

Seokhee Kim

Publication

Eom et al Nucleic Acids Res. 2022 Apr 22;50(7):e38

Insert

EcoRNAPα-PmCDA1

Expression

Bacterial

Promoter

tetR/tetA promoter

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTPH413

Plasmid

#185728

Purpose

Bacterial PAM profiling of adenine base editor variants

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Inserts

ecTadA(8e)-neNme2-C
Nme2Cas9 sgRNA

Use

CRISPR

Expression

Bacterial

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTPH418b

Plasmid

#185725

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEA01

Plasmid

#170766

Purpose

Utilisation of pSAM2 excision tool in Amycolatopsis spp

Depositor

Jean-Luc Pernodet

Publication

Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

Inserts

xis, int
oriT
pac

Expression

Bacterial

Promoter

trcp

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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