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31,182 results

Showing: 6881 - 6900 of 31182 results

  4. pJL1-sfGFP-AQNAT

    Plasmid
    #198211
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing a non-permissible AQNAT sequon followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    sfGFP_AQNAT
    Tags
    6x His Tag and AQNAT mutated (non-permissible) gl…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198211-plasmid-map-sequence-id-393679

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  5. pJL1-sfGFP-MOOR

    Plasmid
    #198212
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing the minimum optimal O-linked recognition site (MOOR) followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    sfGFP_MOOR
    Tags
    6x His Tag and MOOR glycosylation tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198212-plasmid-map-sequence-id-393678

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  10. pOPINN-GFP

    Plasmid
    #53541
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Tags
    N-His-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53541-plasmid-map-sequence-id-83419

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  13. HisMS2_VLP_RSV

    Plasmid
    #175958
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]
    Depositor
    Paul Freemont
    Article
    Unruh et al medRxiv 2024.04.17.24305965
    Inserts
    P gene
    M gene
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    175958-plasmid-map-sequence-id-357255

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  16. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162681-plasmid-map-sequence-id-321444

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  18. pWJ68

    Plasmid
    #46571
    Purpose
    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)
    Depositor
    Luciano Marraffini
    Article
    Bikard et al Nucleic Acids Res. 2013 Jun 12.
    Inserts
    tracrRNA
    w-dcas9
    CRISPR array
    Use
    CRISPR
    Tags
    rpoZ
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    46571-plasmid-map-sequence-id-65847

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Showing: 6881 - 6900 of 31182 results