33,449 results
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Plasmid#174503Purposeexpress P53 DNA binding domain (94-312) in E.coli without tagDepositorInsertP53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
TagsnoneExpressionBacterialPromoterT7Available sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBx1-VHH template3-3XMyc-Spacer
Plasmid#176210PurposePCR template for generating CeVICA VHH/nanobody libraryDepositorInsertVHH template3-3XMyc-Spacer
TagsMyc tagExpressionBacterialPromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-msfGFP
Plasmid#174492Purposebacterial expression of human SEPT2 fused to monomeric superfolder GFPDepositorAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_C5
Plasmid#171925PurposeBacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2DepositorInsertVHH_C5
TagsHis6ExpressionBacterialAvailable sinceSept. 23, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAT9679-sgRNA-BFP
Plasmid#162988PurposesgRNA cloning plasmidDepositorInsertexchangeable cassette
UseCRISPRExpressionBacterial and MammalianPromoterU6Available sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SUMO-AsCas12f
Plasmid#171612PurposeAsCas12f1 heterologous expression plasmidDepositorInsertAsCas12f1
ExpressionBacterialAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB207
Plasmid#164122PurposeJ23101 driving PP7 repeatsDepositorInsertYFP-PP7
ExpressionBacterialPromoterJ23101Available sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBad-UGAcS
Plasmid#172137Purposebacteria expression plasmid for a UDP-GlcNAc sensorDepositorInsertUGAcS
ExpressionBacterialMutationH605Q- please see depositor commentsPromoteraraBADAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41
Plasmid#172757PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-41
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBad-UXPS
Plasmid#172138Purposebacteria expression plasmid for a UDP/UTP sensorDepositorInsertUXPS
ExpressionBacterialPromoteraraBADAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7SS-DogTag
Plasmid#171775PurposeExpresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formationDepositorInsertHaloTag7SS-DogTag
TagsHis6ExpressionBacterialMutationDogTag inserted in HaloTag7 between residues D139…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-His6-TEV_SacB
Plasmid#164588PurposeBacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.DepositorInsertTEV-SacB
TagsTrx-His6 followed by TEV siteExpressionBacterialPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTS_TXS
Plasmid#163839PurposeiPTG inducible expression of taxadiene synthase and geranylgeranyl diphosphate synthaseDepositorInserttaxadiene synthase (61-862)
ExpressionBacterialPromoterTrcAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBJ3
Plasmid#167132PurposeA kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterFrr promoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGV3341
Plasmid#170279PurposeExpression of mutant HIV-2 RT p66 reverse transcriptaseDepositorInsertHIV-2 RT p66 (mutant)
ExpressionBacterialPromoterT7-lacAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS29: pDonor(PmcCAST)
Plasmid#168162PurposePmcCAST donor harboring non-functional cargo.DepositorInsertsPmcCAST right end (RE)
PmcCAST left end (LE)
ExpressionBacterialAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNIC-AncCAR_A
Plasmid#168286PurposeExpresses AncCAR_A in E. coli from a T7 promoterDepositorInsertAncCAR_A
UseSynthetic BiologyTags6*HisExpressionBacterialPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPM2213
Plasmid#167553PurposeEncodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12DepositorInsertrfb region of Shigella flexneri serotype 2a
ExpressionBacterialAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)
Plasmid#169079PurposeHuman ATG3DepositorInsertATG3 (ATG3 Human)
Tags6X Histidine Tag, TEV cleavage siteExpressionBacterialPromoterT7 lac promoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by