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Bacterial Expression Clear filter

32,218 results

Showing: 10411 - 10440 of 32218 results

  5. AKAP13

    Plasmid
    #73232
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    AKAP13 (AKAP13 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 1976-2211
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid
    #112031
    Purpose
    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    Tags
    Split mCherry
    Expression
    Bacterial
    Available Since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112031-plasmid-map-sequence-id-216275

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  14. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162682-plasmid-map-sequence-id-320836

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  16. pGEX2TK-Osh2-PH

    Plasmid
    #71370
    Purpose
    bacterial expression of PH domain for phosphoinositide binding assay
    Depositor
    Mark Lemmon
    Article
    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.
    Insert
    Osh2-PH
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Pleckstrin homology (PH) domain; aa 277-398
    Promoter
    tac
    Available Since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  17. pAV10.KN

    Plasmid
    #63209
    Purpose
    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63209-plasmid-map-sequence-id-115799

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  19. 4Z3C (TET3)

    Plasmid
    #119969
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    TET3 (TET3 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 48-98
    Promoter
    T7
    Available Since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119969-plasmid-map-sequence-id-232693

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  20. INMT

    Plasmid
    #25475
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    INMT (INMT Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  25. pBT3024

    Plasmid
    #122542
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_mt::CemOrange2
    Tags
    MLSLRQSIRFFKPATRTLCSSRTLL
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122542-plasmid-map-sequence-id-238125

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  26. 883 pET15b PTEN

    Plasmid
    #10936
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  27. pSA120

    Plasmid
    #59789
    Purpose
    C. elegans heat-shock driven ZIF-1 and mCherry co-expressed in the same operon
    Depositor
    Jeremy Nance
    Article
    Armenti et al Development. 2014 Dec;141(23):4640-7. do
    Inserts
    hsp-16.41 promoter
    zif-1 coding
    gpd-2/gpd-3 intergenic sequence
    mCherry (worm)
    unc-54 3' UTR
    Expression
    Bacterial
    Available Since
    Oct. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59789-plasmid-map-sequence-id-95470

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Showing: 10411 - 10440 of 32218 results