34,148 results
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Plasmid#51056PurposeExpress self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET30-R5-GFP
Plasmid#17838DepositorInsertpET30-GFP
UseSynthetic BiologyTagsHis, R5, and S-tagExpressionBacterialAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
GLI-3 bs-2
Plasmid#16420DepositorInsertGLI3 (GLI3 Human)
ExpressionBacterialAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody
Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
Tags14xHis-Avi-SUMOEuExpressionBacterialPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNeae2
Plasmid#168300PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
Tags6xHis, E-tag, and myc-tagExpressionBacterialPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
6His-MBP-TEV-huAsCpf1
Plasmid#90095PurposeBacterial expression plasmid for protein purificationDepositorInserthuAsCpf1
Tags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKCcas9dO
Plasmid#62552PurposeHigh efficiency Streptomyces genome editing by CRISPR/Cas9 systemDepositorInsertsScocas9
sgRNA ()
act-orf4 homology arm
UseCRISPR; Bacterial genome editingExpressionBacterialPromoterj23119 and ptipAAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MP4
Plasmid#69652PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA
ExpressionBacterialPromoterE. coli PBADAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV LIC cloning vector (2B-T)
Plasmid#29666DepositorTypeEmpty backboneTagsHis6-TEVExpressionBacterialAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET mOrange LIC cloning vector (u-mOrange)
Plasmid#29770DepositorTypeEmpty backboneTagsmOrangeExpressionBacterialAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDEST17-UbE2L3
Plasmid#15795DepositorPublicationAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pATetO 6XHis-Phusion
Plasmid#206210PurposeInducible expression of DNA polymeraseDepositorInsertPhusion DNA polymerase
ExpressionBacterialAvailable sinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf17-22
Plasmid#198272PurposeUnmodified pPayload; contains WT PVC payloads/regulatory genesDepositorInsertPVCpnf17-22
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSL0283 (pTnsABC)
Plasmid#130633PurposeExpresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.DepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonExpressionBacterialPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCold-I-Dsup
Plasmid#90021PurposeTo express His-tagged Dsup in bacteriaDepositorInsertDsup
TagsHisExpressionBacterialPromotercspAAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNIC28-DENV3-NS5FL
Plasmid#86013PurposeExpression of dengue virus 3 NS5 proteinDepositorInsertDENV3-NS5FL
Tagshexa-histidine tagExpressionBacterialMutationamino acid residues 6 to 895 of DENV3 NS5PromoterT7Available sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHMM
Plasmid#67717PurposeExpression vector MBP/hexahistidine tagged variant of the MS2 coat proteinDepositorInsertHexahistidine/Maltose binding protein/MS2 coat protein fusion
TagsHexahistidine tagExpressionBacterialPromoterT7 RNA polymeraseAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
TagsSpyCatcher and SpyTagExpressionBacterialPromoterT7 promoterAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-5X-TRIM28
Plasmid#45570DepositorAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and NonprofitsCited by