29,981 results

1. pJ1996_v2

   Plasmid

   #140664

   Purpose

   dCas9 & Csy4

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   dCas9 & Csy4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pABE8e-protein

   Plasmid

   #161788

   Purpose

   Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

   Depositor

   David Liu

   Article

   Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

   Insert

   ABE8e base editor
   

   Use

   Tags

   8xHIS and BPNLS

   Expression

   Bacterial

   Mutation

   Promoter

   pRhaBAD

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-2TK-14-3-3 beta GST

   Plasmid

   #13276

   Depositor

   Michael Yaffe

   Article

   Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

   Insert

   14-3-3 beta (YWHAB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pHen2 Avi-tag-linker-p3

   Plasmid

   #119819

   Purpose

   For cloning and subsequent expression of peptide fragments fused with Avi-tag (for biotinylation) and with pIII protein of M13K07 bacteriophage

   Depositor

   Alexander Gabibov

   Article

   Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

   Insert

   linker with Avi-tag and p3
   

   Use

   Tags

   Avi-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pE-FLP

   Plasmid

   #45978

   Purpose

   Expresses yeast Flp driven by the constitutive promoter pE from phage P2

   Depositor

   Keith Shearwin, Drew Endy

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Insert

   flp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   flp driven by the constitutive promoter pE from p…

   Promoter

   pE (from phage P2)

   Available since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET29b_mNeonGreen

   Plasmid

   #209425

   Purpose

   Express mNeonGreen in E. coli

   Depositor

   Magnus Rueping

   Article

   Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

   Insert

   mNeonGreen
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGRG36

   Plasmid

   #16666

   Depositor

   Nancy Craig

   Article

   McKenzie et al BMC Microbiol. 2006 . 6():39.

   Insert

   mTn7::MCS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-4T1-14-3-3 zeta GST

   Plasmid

   #13278

   Depositor

   Michael Yaffe

   Article

   Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

   Insert

   14-3-3 zeta (YWHAZ Bovine)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pGEX-5X1 Flag PA1

   Plasmid

   #15560

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   PA1 (PAGR1 Human)
   

   Use

   Tags

   Flag and GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pEGFP-C3-hYAP1

    Plasmid

    #17843

    Depositor

    Marius Sudol

    Article

    Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKL001

    Plasmid

    #98917

    Purpose

    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    CaPR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGDP3 apmA

    Plasmid

    #112881

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    apmA (apmA Staphylococcus aureus)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Pbla

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKEW302-AcrVA4

    Plasmid

    #115656

    Purpose

    Expresses AcrVA4 in bacteria, under TetR control.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-28a+GFP-CAMSAP3 CKK

    Plasmid

    #59033

    Purpose

    expresses CKK domain of CAMSAP3 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP3 CKK domain (CAMSAP3 Mouse)
    

    Use

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pOTIII.EFGP.IGR3.6

    Plasmid

    #40866

    Depositor

    Harold Gainer

    Article

    Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

    Insert

    MOUSE OXYTOCIN GENE (Oxt Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mouse oxytocin promoter

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKEW303-AcrVA5

    Plasmid

    #115657

    Purpose

    Expresses AcrVA5 in bacteria, under TetR control.

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKEW189-MBP-TEV-AcrVA1

    Plasmid

    #115660

    Purpose

    Expresses AcrVA1 in bacteria via T7 RNAP, for purification

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA1
    

    Use

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNAP

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AcrIIA2

    Plasmid

    #101042

    Purpose

    Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

    Depositor

    Zhiwei Huang

    Article

    Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

    Insert

    AcrIIA2
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK645

    Plasmid

    #71700

    Purpose

    Produces E. coli thioredoxin (EcTrxA)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge