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pET28a-MSP1D1deltaH4H5

Plasmid

#71716

Purpose

Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

Depositor

Gerhard Wagner

Publication

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP

Plasmid

#51056

Purpose

Express self-cleaving-ribozyme-flanked sgRNA cassette (RGR) targeting GFP for CRISPR systems in yeast driven by ADH1 promoter. RGR has a HH ribozyme at its 5', and an HDV ribozyme at its 3'.

Depositor

Yunde Zhao

Publication

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET His6 TEV LIC cloning vector (2B-T)

Plasmid

#29666

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6-TEV

Expression

Bacterial

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pET mOrange LIC cloning vector (u-mOrange)

Plasmid

#29770

Depositor

Scott Gradia

Type

Empty backbone

Tags

mOrange

Expression

Bacterial

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBR322-PVCpnf17-22

Plasmid

#198272

Purpose

Unmodified pPayload; contains WT PVC payloads/regulatory genes

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf17-22

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pNeae2

Plasmid

#168300

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Publication

Salema et al PLoS One. 2013 Sep 23;8(9):e75126. doi:

Insert

Enterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus

Tags

6xHis, E-tag, and myc-tag

Expression

Bacterial

Promoter

LacI-Plac

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDB302

Plasmid

#113673

Purpose

expresses Cth SUMO protease in Escherichia coli

Depositor

Christopher Bahl

Publication

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Cth SUMO protease

Tags

10-His

Expression

Bacterial

Mutation

deleted amino acids 1-934

Promoter

T7

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pNIC28-DENV3-NS5FL

Plasmid

#86013

Purpose

Expression of dengue virus 3 NS5 protein

Depositor

Dahai Luo

Publication

Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

Insert

DENV3-NS5FL

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

amino acid residues 6 to 895 of DENV3 NS5

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pKCcas9dO

Plasmid

#62552

Purpose

High efficiency Streptomyces genome editing by CRISPR/Cas9 system

Depositor

Weihong Jiang

Publication

Huang et al Acta Biochim Biophys Sin (Shanghai). 201

Inserts

Scocas9
sgRNA ()
act-orf4 homology arm

Use

CRISPR; Bacterial genome editing

Expression

Bacterial

Promoter

j23119 and ptipA

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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MP4

Plasmid

#69652

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Publication

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA

Expression

Bacterial

Promoter

E. coli PBAD

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-TRIM28

Plasmid

#45570

Depositor

Fanxiu Zhu

Publication

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Insert

TRIM28 (TRIM28 Human)

Tags

GST

Expression

Bacterial

Promoter

Ptac

Available since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pMV306G13+Lux

Plasmid

#26160

Depositor

Brian Robertson, Siouxsie Wiles

Publication

Andreu et al PLoS One. 2010 May 24;5(5):e10777.

Insert

G13 promoter + Bacterial luciferase operon

Expression

Bacterial

Mutation

It contains a gram-positive enhanced translation …

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GST-Hsp90 N(9-236)

Plasmid

#22481

Depositor

William Sessa

Publication

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90 alpha N domain (HSP90AA1 Human)

Tags

GST

Expression

Bacterial

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pDEST17-UbE2L3

Plasmid

#15795

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK 14-3-3 sigma GST

Plasmid

#11944

Depositor

Michael Yaffe

Publication

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 sigma (SFN Human)

Tags

GST

Expression

Bacterial

Available since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDuEx2-NIR-GECO1

Plasmid

#113680

Purpose

Bacterial and mammalian expression plasmid for near-infrared fluorescent Ca2+ indicator NIR-GECO1

Depositor

Robert Campbell

Publication

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NIR-GECO1

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJYS2_crtYf

Plasmid

#85544

Purpose

Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

Depositor

Sheng Yang

Publication

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Insert

crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET21b-Caspase-3

Plasmid

#90087

Purpose

Expresses wild type caspase 3 in bacterial cells

Depositor

Clay Clark

Publication

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

Wild type

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHMM

Plasmid

#67717

Purpose

Expression vector MBP/hexahistidine tagged variant of the MS2 coat protein

Depositor

Robert Batey, Jeffrey Kieft

Publication

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Hexahistidine/Maltose binding protein/MS2 coat protein fusion

Tags

Hexahistidine tag

Expression

Bacterial

Promoter

T7 RNA polymerase

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSR33.4r

Plasmid

#63198

Purpose

Expresses Cph8, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Publication

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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