32,871 results
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Plasmid#110274PurposeElongin B and Elongin C co-expression plasmid for structure determination; Full length Elongin B & amino acids 17-112 of Elogin CDepositorExpressionBacterialMutationElongin B is full length. Elongin C contains a.a…PromoterT7Available SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only
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pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
Tags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKCcas9dO
Plasmid#62552PurposeHigh efficiency Streptomyces genome editing by CRISPR/Cas9 systemDepositorInsertsScocas9
sgRNA ()
act-orf4 homology arm
UseCRISPR; Bacterial genome editingExpressionBacterialPromoterj23119 and ptipAAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-TRIM28
Plasmid#45570DepositorAvailable SinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET His6 TEV LIC cloning vector (2B-T)
Plasmid#29666DepositorTypeEmpty backboneTagsHis6-TEVExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMV306G13+Lux
Plasmid#26160DepositorInsertG13 promoter + Bacterial luciferase operon
ExpressionBacterialMutationIt contains a gram-positive enhanced translation …Available SinceFeb. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
GST-Hsp90 N(9-236)
Plasmid#22481DepositorAvailable SinceApril 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2L3
Plasmid#15795DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK 14-3-3 sigma GST
Plasmid#11944DepositorAvailable SinceMay 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
HP4_M13
Plasmid#120340PurposeHelper plasmid for production of phagemid ssDNA, based on m13mp18DepositorInsertCoding part of the M13 phage genome
ExpressionBacterialPromoternative phage promotersAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-DENV3-NS5FL
Plasmid#86013PurposeExpression of dengue virus 3 NS5 proteinDepositorInsertDENV3-NS5FL
Tagshexa-histidine tagExpressionBacterialMutationamino acid residues 6 to 895 of DENV3 NS5PromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR33.4r
Plasmid#63198PurposeExpresses Cph8, Ho1, and PcyA constitutivelyDepositorInsertsCph8
Ho1
PcyA
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
TagsSpyCatcher and SpyTagExpressionBacterialPromoterT7 promoterAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKD‐SepRS-EFSep-5x tRNASep (B40 OTS)
Plasmid#52054PurposeUsed in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)DepositorInsertsSepRS
EF-Sep
5x tRNA Sep
ExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtacAvailable SinceMarch 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmOrange
Plasmid#37129DepositorInsertLSSmOrange
TagsHisExpressionBacterialMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoteraraBAvailable SinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
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LB322 (Bcl-2 from ATG to -3934)
Plasmid#15381DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from ATG to -3934 with both P1 and…Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-2T-Rac1-wt
Plasmid#12977DepositorAvailable SinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBR322-PVCpnf17-22
Plasmid#198272PurposeUnmodified pPayload; contains WT PVC payloads/regulatory genesDepositorInsertPVCpnf17-22
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only