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GST-p35

Plasmid

#24895

Depositor

Harish Pant

Article

Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

Insert

p35 (CDK5R1 Human)

Tags

GST

Expression

Bacterial

Mutation

Full-length p35

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pBLind

Plasmid

#113109

Purpose

Vector encoding the RFP gene under blue light-inducible promoter BindF1.

Depositor

Chueh Loo Poh

Article

Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

Insert

RFP

Use

Cell-free

Expression

Bacterial

Promoter

BindF1

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pIBA3-SpyCatcher-sfGFP

Plasmid

#107420

Purpose

For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

Depositor

Jack Leo

Article

Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

Insert

SpyCatcher

Tags

His tag, StrepII, and superfolder GFP

Expression

Bacterial

Promoter

Tet

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-CmCHS-MsCHI

Plasmid

#73394

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPSU20-40-60-80

Plasmid

#173839

Purpose

Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-1β

Plasmid

#214340

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL1B (IL1B Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCK01_PalkS_alkS

Plasmid

#166502

Purpose

Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.

Depositor

Sven Panke

Article

Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

Insert

alkS

Use

Synthetic Biology

Expression

Bacterial

Mutation

L401G

Promoter

PalkS

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTE105

Plasmid

#59400

Purpose

MethyloBrick expression vector, tuf promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptuf

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pQE80L-SpyCatcher-ELP-GFP

Plasmid

#69835

Purpose

Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

Insert

SpyCatcher-ELP-GFP

Tags

6x His Tag, GFP, and TEV Tag

Expression

Bacterial

Promoter

T5 promoter/lac operator element

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEN019-SpCas9-hiNLS-t-M1M4

Plasmid

#234456

Purpose

Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

Depositor

Ross Wilson

Article

Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

Insert

SpCas9 hiNLS t-M1M4

Use

CRISPR

Tags

His6, Nucleoplasmin, SV40, and c-Myc

Expression

Bacterial

Mutation

Cys at positions 80 and 574 have been mutated to …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET3a-hUBA1

Plasmid

#63571

Purpose

Expression of human UBA1 tagged with His

Depositor

Titia Sixma

Article

Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

Insert

UBA1 (UBA1 Human)

Expression

Bacterial

Mutation

Isoform 2 (1018 amino acids)

Promoter

T7

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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pSUP-(2xCouRS-tRNAopt)

Plasmid

#197930

Purpose

Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

Depositor

Kenneth Johnson

Article

Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

Inserts

CouRS
tRNA opt

Expression

Bacterial

Promoter

glnS' and lambda(ts) and proK

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR

Plasmid

#109055

Purpose

Bacterial expression of E.coli DHFR protein

Depositor

Stephen Benkovic

Article

Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

Insert

ecDHFR

Expression

Bacterial

Promoter

T7

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pJG103

Plasmid

#158533

Purpose

wrmScarlet11 x3 tandem repeats

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet11 x3 tandem repeats

Expression

Bacterial

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pJOE7706.1

Plasmid

#135075

Purpose

A shuttle vector for heterologous HA production with Corynebacterium glutamicum

Depositor

Josef Altenbuchner

Article

Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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DP6

Plasmid

#140446

Purpose

Untargetted bacterial drift

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1 gIII

Expression

Bacterial

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1

Plasmid

#187830

Purpose

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Tags

GST and TEV-mEGFP-GG Linker

Expression

Bacterial and Insect

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pTEF2

Plasmid

#43971

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

TEF

Available Since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a(His10-PD-1cyto)

Plasmid

#177896

Purpose

E coli expression of His10 tag fused to human PD-1 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

PD-1 (aa 194-288 only) (PDCD1 Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2024

Availability

Academic Institutions and Nonprofits only

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