30,735 results

1. pSIP403

   Plasmid

   #122028

   Purpose

   Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

   Depositor

   Geir Mathiesen, Lars Axelsson

   Article

   Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

   Insert

   gusA
   

   Expression

   Bacterial

   Promoter

   sppA

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJLG038

   Plasmid

   #192981

   Purpose

   BHR plasmid (pBBR1 ori) expressing T7 polymerase under control of the lac promoter

   Depositor

   Clay Fuqua

   Article

   Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

   Insert

   T7 polymerase
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTP264

   Plasmid

   #149334

   Purpose

   Bacterial expression of Biotin ligase BirA

   Depositor

   Dirk Görlich

   Article

   Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

   Insert

   Biotin ligase BirA
   

   Tags

   His14 and bdNEDD8

   Expression

   Bacterial

   Available Since

   June 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SpyTag003-sfGFP

   Plasmid

   #133454

   Purpose

   Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-sfGFP
   

   Tags

   GSGSGS linker and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28:GFP

   Plasmid

   #60733

   Purpose

   pET28 driving expression of GFP, biobrick part Bba_E0040

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJMK001

   Plasmid

   #33780

   Depositor

   Adam Cohen

   Article

   Kralj et al Science. 2011 Jul 15;333(6040):345-8.

   Insert

   Proteorhodopsin Optical Proton Sensor
   

   Expression

   Bacterial

   Mutation

   D97N

   Promoter

   AraBAD

   Available Since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMAL-XPG

   Plasmid

   #39215

   Depositor

   Aziz Sancar

   Article

   Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

   Insert

   XPG (ERCC5 Human)
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Note that XPG starts at amino acid 5

   Promoter

   Ptac

   Available Since

   Aug. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET 28 full-length human PARP1

   Plasmid

   #192635

   Purpose

   Expresses human PARP1 with an N-terminal His-tag

   Depositor

   John Pascal

   Article

   Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

   Insert

   Human PARP1 (PARP1 Human)
   

   Tags

   histidine-tag

   Expression

   Bacterial

   Mutation

   Polymorphism A762

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMevT

   Plasmid

   #17815

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKDsgRNA-ack

    Plasmid

    #62654

    Purpose

    Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    exo, beta, gam, sgRNA
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHN924

    Plasmid

    #178885

    Purpose

    Expression of GST-His8-HRV3C

    Depositor

    Kazuhiro Abe

    Insert

    human rhinovirus 3C protease
    

    Tags

    GST, His8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SuperNova Green/ pRSETB

    Plasmid

    #124839

    Purpose

    bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

    Insert

    SuperNova Green
    

    Tags

    No tag

    Expression

    Bacterial

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30-2-GAPDH

    Plasmid

    #83910

    Purpose

    stable overexpression

    Depositor

    David Sabatini

    Article

    Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

    Insert

    glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)
    

    Tags

    6x His tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28-His-TEV-NQO1

    Plasmid

    #119058

    Purpose

    For expression in E.coli and affinity purification of N-terminally His tagged NQO1

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Tags

    6His, TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-6xHis-MBP-TEV site-TadA8r

    Plasmid

    #208318

    Purpose

    Overxpression MBP-TadA8r

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r
    

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET51b-His-TEV-CLIP-tag

    Plasmid

    #167274

    Purpose

    Overexpression of CLIP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    CLIP-tag
    

    Tags

    His x10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTNT-B18R

    Plasmid

    #58978

    Purpose

    expresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    B18R
    

    Expression

    Bacterial

    Promoter

    T7/Sp6

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RP1B FUS 1-163

    Plasmid

    #127192

    Purpose

    Protein expression for affinity purification

    Depositor

    Nicolas Fawzi

    Article

    Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

    Insert

    FUS 1-163 (FUS Human)
    

    Tags

    hexahistidine

    Expression

    Bacterial

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDae069 (eMutaT7[PmCDA1] with optimized RBS)

    Plasmid

    #187621

    Purpose

    Expresses eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

    Insert

    PmCDA1-T7RNAP
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge