30,256 results

1. pet-MBP

   Plasmid

   #209184

   Purpose

   Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Type

   Empty backbone

   Use

   Tags

   HisX6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1142 (pSPIN, pBBR1 backbone)

   Plasmid

   #160730

   Purpose

   Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSF-G1(7FTrp)RS

   Plasmid

   #177310

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al ACS Sens. 2022 Jan 10. doi: 10.1021/acss

   Insert

   tRNA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe-tetoff-mVenus-PICdc42

   Plasmid

   #91876

   Purpose

   PI(WT): Photo inhibitable (blue light sensitive)

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Insert

   Vav2 (VAV2 Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. LM670

   Plasmid

   #191552

   Purpose

   LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

   Depositor

   David Baker

   Article

   Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

   Insert

   ccdb
   

   Use

   Tags

   HIS-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. UbcH5c S22R pET28a

   Plasmid

   #12644

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   Ubch5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

7. TDP-43_CTD

   Plasmid

   #98669

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. 12.4kbVillin-ΔATG

   Plasmid

   #19358

   Depositor

   Deborah Gumucio

   Article

   Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

   Insert

   12.4kb Villin promoter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No ATG - transgenes cloned downstream of the vill…

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-BrCas12b-RFND

    Plasmid

    #195339

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis and 6xHis, MBP, TEV cleavage site

    Expression

    Bacterial

    Mutation

    R160E, F208W, N524V, D868V

    Promoter

    T7

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid

    #72325

    Purpose

    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-mEGFP-SpyTag
    

    Use

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP396

    Plasmid

    #149336

    Purpose

    Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

    Depositor

    Rebecca Voorhees

    Article

    Pleiner et al Science. 2020 May 21. pii: science.abb50

    Insert

    anti-GFP nanobody
    

    Use

    Tags

    Biotin acceptor peptide (Avi-tag), His14, and SUM…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28a-mCherry-MED1-IDR

    Plasmid

    #194545

    Purpose

    Bacterial Expression of 6xHis mCherry-MED1-IDR Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    MED1 (MED1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. VHL-pGex2TK

    Plasmid

    #20790

    Depositor

    William Kaelin

    Article

    Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

    Insert

    VHL (VHL Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    VHL wt cDNA, no mutations

    Promoter

    Available since

    April 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-45b-POLR2A-CTD-mCherry

    Plasmid

    #185011

    Purpose

    Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    POLR2A (Polr2a Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6xHis-SNAP-PI Probe

    Plasmid

    #211507

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BcPI-PLC-ANH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HC-M

    Plasmid

    #173481

    Purpose

    An rrnB P1-based GFP ATP reporter in E. coli

    Depositor

    Rahul Sarpeshkar

    Article

    Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

    Insert

    GFP-mut2
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    rrnB P1

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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