30,327 results

1. R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

   Plasmid

   #159689

   Purpose

   E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

   Depositor

   Dominic Esposito

   Article

   Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)
   

   Use

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTCF120 H-2D(b)

   Plasmid

   #180454

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   H-2D(b)
   

   Use

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Promoter

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P1 METTL3

   Plasmid

   #200731

   Purpose

   Bacterial expression of GST-Tagged codon optimized human METTL3

   Depositor

   Claudio Alarcón

   Insert

   METTL3 (METTL3 Human)
   

   Use

   Tags

   GST from backbone plasmid

   Expression

   Bacterial

   Mutation

   Codon optimized for expression in E. coli

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-7 asyn G51D

   Plasmid

   #105747

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Fares et al Hum Mol Genet. 2014 Sep 1;23(17):4491-50

   Insert

   alpha-synuclein G51D (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G51D

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. 14x PP7 with loxP-kanMX-loxP

   Plasmid

   #189939

   Purpose

   Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

   Depositor

   Tineke Lenstra

   Article

   Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   14x PP7 with loxP-kanMX-loxP
   

   Use

   Tags

   kanMX

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGM190

   Plasmid

   #69614

   Purpose

   inducible gene expression in Streptomyces

   Depositor

   Günther Muth

   Article

   Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

   Inserts

   aphII
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Use

   Streptomyces- e. coli shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSB1C3-pT3-tetR-sfGFP

   Plasmid

   #140871

   Purpose

   T3 promoter driven synthesis of TetR-sfGFP

   Depositor

   Neal Devaraj

   Article

   Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

   Insert

   TetR-sfGFP expressed with T3 promoter
   

   Use

   Synthetic Biology

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T3

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T-3-SpSuc1

   Plasmid

   #162558

   Purpose

   Expresses GST-tagged S.pombe Suc1 in E.coli

   Depositor

   Iain Hagan

   Article

   Singh et al Methods Mol Biol. 2021;2329:123-142. doi

   Insert

   Schizosaccharomyces pombe Suc1 (suc1 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 5T5G (SETD8)

   Plasmid

   #101886

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SETD8 (KMT5A Human)
   

   Use

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pTAT2.2

    Plasmid

    #35621

    Depositor

    Steven Dowdy

    Article

    Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

    Type

    Empty backbone

    Use

    Tags

    6xHIS, T7 tag, and TAT

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. SUMO-KTag

    Plasmid

    #51723

    Purpose

    Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag

    Depositor

    Mark Howarth

    Article

    Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

    Insert

    SUMO-KTag
    

    Use

    Tags

    His6 Tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pBAD-TDsmURFP-RBS-HO-1

    Plasmid

    #80342

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Use

    Tags

    6 Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-mPA-GFP-PA-Rac1

    Plasmid

    #22022

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His and mPA-GFP

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

14. human p53-(1-360)

    Plasmid

    #24865

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1-360.

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

15. MBP-C5E-6XTUBE

    Plasmid

    #217628

    Purpose

    Bacterial expression of MBP tagged 6XTUBE

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    6XTUBE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTD-C_eYFPTwinStrep

    Plasmid

    #45942

    Purpose

    expresses E.coli codon optimized EYFP-Twin-Strep fusion protein

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic eYFP (codon usage adapted to E.coli K12)
    

    Use

    Tags

    Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq/Ptrc

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWT021a

    Plasmid

    #107895

    Purpose

    W2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    PTetO-SD8-BE2, PLac-sgRNA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWaldo-GFPe_PepTSo

    Plasmid

    #58334

    Purpose

    Expresses the POT family transporter from Shewanella oniedensis in E. coli as a C-terminally tagged GFP his tagged protein

    Depositor

    Simon Newstead, So Iwata

    Article

    Newstead et al EMBO J. 2011 Jan 19;30(2):417-26. doi: 1

    Insert

    PepTso
    

    Use

    Tags

    GFP-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. His10-PS-SNAPf (XSB522)

    Plasmid

    #78512

    Purpose

    Construct for expressing His10 - SNAPf in E. Coli

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    SNAPf
    

    Use

    Tags

    His10 followed by a prescission protease claevage…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. MZB206

    Plasmid

    #228493

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fused Avi-tag, HRV-3C cleavable MSP2N2 for biotinylated nanodiscs

    Depositor

    Richard Baker

    Insert

    MSP2N2
    

    Use

    Tags

    10xHis-SUMO(Smt3)-Avitag-HRV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge