30,808 results

1. pUC-UAS1B16-TEF(272)

   Plasmid

   #44375

   Depositor

   Hal Alper

   Article

   Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

   Insert

   UAS1B16-TEF(272) promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   UAS1B16-TEF(272)

   Available Since

   April 18, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pOPARA1

   Plasmid

   #200416

   Purpose

   Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)

   Depositor

   Kamil Witek

   Article

   Lesley et al bioRxiv

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBEL2511

   Plasmid

   #195625

   Purpose

   Complementation of MlaA(WT)

   Depositor

   Gira Bhabha, Damian Ekiert

   Article

   MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

   Insert

   MlaA
   

   Expression

   Bacterial

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. A1-LCD+7R+12D

   Plasmid

   #178678

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with seven Arg residues added, twelve Glu residues added (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD+7R+12D (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   seven Arg residues added, twelve Glu residues add…

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SAP130

   Plasmid

   #11241

   Depositor

   Robin Reed

   Article

   Das et al Mol Cell Biol. 1999 Oct . 19(10):6796-80

   Insert

   SAP130 (SF3B3 Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pDEST17-cDAXX

   Plasmid

   #169729

   Purpose

   Bacterial expression of N-terminally 6His tagged cDAXX

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   DAXX (DAXX Human)
   

   Tags

   6 His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEDJ333

   Plasmid

   #177272

   Purpose

   GAL1p:Cas9:CYC1t

   Depositor

   Michael Jensen

   Article

   Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

   Insert

   Cas9
   

   Expression

   Bacterial and Yeast

   Promoter

   GAL1

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSW002-PpsbA-DsRed-Express2

   Plasmid

   #111257

   Purpose

   Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   DsRed-Express2
   

   Expression

   Bacterial

   Promoter

   PpsbA

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKB817 (pBra-Hfq)

   Plasmid

   #174661

   Purpose

   Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli Hfq. Useful as three-hybrid positive control.

   Depositor

   Katherine Berry, Ann Hochschild

   Article

   Berry et al Nucleic Acids Res. 2018 Jan 25;46(2):e12

   Insert

   Hfq
   

   Tags

   alpha NTD

   Expression

   Bacterial

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBR322-Pdp1_NTD-ZFD_L

    Plasmid

    #198276

    Purpose

    For loading L half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Left-G1397-N_DddA); also contains PVC regulatory genes

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Pdp1_NTD-ZFD_L
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBS human IRS-1

    Plasmid

    #11359

    Depositor

    Ronald Kahn

    Article

    Araki et al Diabetes. 1993 Jul . 42(7):1041-54.

    Insert

    IRS-1 (IRS1 Human)
    

    Expression

    Bacterial

    Available Since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJL1 Aquamarine

    Plasmid

    #106285

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    Aquamarine
    

    Tags

    6xHis and TEV cleavage (N terminal on insert)

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKB01_tagRFP

    Plasmid

    #69103

    Purpose

    Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    tagRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKJ1712

    Plasmid

    #21870

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Protoc. 2009. 4(10):1471-501.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-jRCaMP1a

    Plasmid

    #113936

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    jRCaMP1a
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-mF(3230)A3

    Plasmid

    #168016

    Purpose

    Expresses MBP-F(3230)A3-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-F(3230)A3-6xHis
    

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. EP300

    Plasmid

    #39018

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    EP300 (EP300 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCRII p15

    Plasmid

    #16454

    Depositor

    Bert Vogelstein

    Article

    Jen et al Cancer Res. 1994 Dec 15. 54(24):6353-8.

    Insert

    p15 (CDKN2B Human)
    

    Expression

    Bacterial

    Available Since

    March 10, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pCoofy3

    Plasmid

    #43983

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His6-GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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