33,128 results

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pUC19-T7-SdB-T

Plasmid

#87307

Purpose

Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Stabilized dimeric Broccoli aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-Split-Broccoli-Toehold-Switch

Plasmid

#87316

Purpose

Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry

Use

Synthetic Biology

Expression

Bacterial

Promoter

P70a

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm_P1T_DP-B41

Plasmid

#90321

Purpose

Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B41

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Type

Empty backbone

Expression

Bacterial

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pPBC-G6f

Plasmid

#62811

Purpose

pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6f-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - SodA

Plasmid

#89612

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase A

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - TR

Plasmid

#89621

Purpose

[Edit] Expresses recombinant 6HIS tagged T. cruzi trypanothione reductase protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Thioredoxin

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2

Plasmid

#88916

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Expression

Bacterial

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(D3A: D175A D28A D9A)

Plasmid

#90090

Purpose

Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

Depositor

Clay Clark

Article

Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

Insert

Caspase 3 (CASP3 Human)

Tags

His tag

Expression

Bacterial

Mutation

D175A D28A D9A

Available Since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pET22b-csgB

Plasmid

#89582

Purpose

Express csgB curli protein with GS linker and Histaq

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgB (csgB )

Tags

3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

Expression

Bacterial

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET30a-YedK

Plasmid

#86884

Purpose

Bacterial expression of E. coli SRAP ortholog YedK

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

YedK (yedK Eschericia coli)

Tags

6xHis, HA tag, and S-Tag

Expression

Bacterial

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Rac1(WT)-mCherry

Plasmid

#87550

Purpose

PI(WT): Photo-inhibitable/blue light sensitive Rac1

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Rac1 (RAC1 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pYUB28b-MCS-optimized

Plasmid

#89179

Purpose

mycobacteria shuttle vector - pYUB28b with a single change to the MCS

Depositor

Ted Baker, Ghader Bashiri

Article

Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Type

Empty backbone

Use

Mycobacteria shuttle vector

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pYUB28b-MBP

Plasmid

#89178

Purpose

mycobacteria shuttle vector

Depositor

Ted Baker, Ghader Bashiri

Article

Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Type

Empty backbone

Use

Mycobacteria shuttle vector

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4

Plasmid

#87733

Purpose

Bacterial expression of full-length S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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USPL1cat-pETM30

Plasmid

#85768

Purpose

bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

Depositor

Frauke Melchior

Article

Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

Insert

USPL1, catalytic domain (aa 212-514) (USPL1 Human)

Tags

GST and His

Expression

Bacterial

Mutation

catalytic domain (aa 212-514)

Promoter

T7-lac

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDule-IBBN (G2)

Plasmid

#85500

Purpose

Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

Depositor

Ryan Mehl

Article

Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

Insert

IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system

Expression

Bacterial

Mutation

Y32G L65E F108W Q109M D158S R162K

Promoter

lpp (constitutive)

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBluescript-mouse cyclin D2

Plasmid

#86791

Purpose

bacterial expression of mouse cyclin D2

Depositor

Martine Roussel, Charles Sherr

Article

Matsushime et al Cell. 1991 May 17;65(4):701-13.

Insert

cyclin D2 (Ccnd2 Mouse)

Expression

Bacterial

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRAF-MutS

Plasmid

#86605

Purpose

E. coli MutS expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutS (D835R,R840E)

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

D835R, R840E

Promoter

T7

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL2807

Plasmid

#71203

Purpose

AD1 STAR Antisense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

AD1 Antisense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Available Since

Jan. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJBL2801

Plasmid

#71207

Purpose

AD1 Sense Expression Plasmid

Depositor

Julius Lucks

Article

Chappell et al Nat Chem Biol. 2015 Mar;11(3):214-20. do

Insert

AD1 Sense

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23119

Available Since

Jan. 21, 2017

Availability

Academic Institutions and Nonprofits only

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