33,449 results
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Plasmid#103993PurposePlasmid encoding moxVenusDepositorInsertmoxVenus
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable sinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mEmerald
Plasmid#103982PurposePlasmid encoding mEmeraldDepositorInsertmEmerald
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mEGFP
Plasmid#103979PurposePlasmid encoding mEGFPDepositorInsertmEGFP
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, S65T, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZH509
Plasmid#102664PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB036d
Plasmid#99216PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PproAAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP574
Plasmid#98807PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-GST
ExpressionBacterialMutationNoneAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s
Plasmid#100022PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.DepositorInsertsfGCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s-T78H
Plasmid#100023PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant containing T78H mutationDepositorInsertsfGCaMP6s-T78H
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFP and T78H m…PromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
KLHL40 (KBTBD5A-c006)
Plasmid#98233PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)
Plasmid#99343PurposeBacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TADDepositorAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB061i2
Plasmid#79229PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-HA4 (Y87A); PlacIq lacI
ExpressionBacterialPromoterPpro1; PlacIqAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT23
Plasmid#89663Purposeexpression of doubleTAL staple protein dT23 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT23
TagsHis6ExpressionBacterialPromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEThT-SAHS1
Plasmid#90025PurposeTo express His-tagged SAHS1 in bacteriaDepositorPublicationInsertSAHS1
TagsHisExpressionBacterialPromoterT7Available sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L
Plasmid#89610PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416DepositorAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_M337V
Plasmid#98674PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with M337VDepositorAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBEST-p70a-UTR3-deGFP-6xHis-T500
Plasmid#92224PurposeExpression of deGFP using the p70a promoter and UTR3DepositorInsertdeGFP
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b/BOA
Plasmid#82041PurposeFor E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)DepositorInsertBurkholderia oklahomensis agglutinin
Tags6x HisExpressionBacterialAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
mEos3-caRac1
Plasmid#92041PurposeExpresses mEos3 fusion with caRac1 (Q61L)DepositorInsertRAC1 (RAC1 Human)
TagsmEos3ExpressionBacterial, Insect, and Mamm…MutationQ61L (constitutively active)PromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-T7-SdB-T
Plasmid#87307PurposeExpresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.DepositorInsertStabilized dimeric Broccoli aptamer
UseSynthetic BiologyExpressionBacterialPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Split-Broccoli-Toehold-Switch
Plasmid#87316PurposeExpresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.DepositorInsertSplit-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only