32,934 results
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Plasmid#79886PurposeUbiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterUbiquitinAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-cytoBirA-2A-mCherry-SV40pA-FKF
Plasmid#79887PurposeDonor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-flk1-cytoBirA-2A-mCherry_Ras
Plasmid#80056PurposeFlk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
Tags3x HAExpressionBacterialPromoterflk1Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-ro1GFP
Plasmid#82365PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertro1GFP
Tags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204CPromoterT7Available SinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV106
Plasmid#63196PurposepRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV113
Plasmid#63180PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pM
Plasmid#64179Purposeharbours marker gene MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-T7-Ct-YhjH
Plasmid#79167PurposeDual expression of Ct biosensor and YhjH under individual T7 promotersDepositorInsertsyhjH
T7-Spinach2-Ct
ExpressionBacterialPromoterT7Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
HisMBP CFlm25
Plasmid#78458PurposeInducible expression in E. coliDepositorInsertHuman Cleavage Factor lm 25 kDa subunit (NUDT21 Human)
TagsHis and MBPExpressionBacterialPromotertacAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c4E
Plasmid#75293PurposeExpresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding proteinDepositorTypeEmpty backboneTagsmaltose-binding proteinExpressionBacterialPromotertacAvailable SinceJuly 19, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLC-239
Plasmid#73193PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorAvailable SinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
His10-CD3z-SNAPf-ybbR (XSB507)
Plasmid#78508PurposeConstruct for expressing CD3z-SNAPf-ybbR in E. ColiDepositorAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
JMJD1CA-c089
Plasmid#79644PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Sos1 (PRM) - Cys (XSB364)
Plasmid#78506PurposeConstruct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labelingDepositorInsertSos1 -Cys (PRM) (SOS1 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLitR-L1
Plasmid#74683PurposeLitR-L1 gateDepositorInsertPTac-RBSL1-LitR-PLitR-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBetI-E1
Plasmid#74677PurposeBetI-E1 gateDepositorInsertPTac-RBSE1-BetI-PBetI-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pDA183
Plasmid#74247PurposepSTL1-dPSTRr(2,1)DepositorInsertpSTL1-dPSTRr(2,1)
ExpressionBacterial and YeastAvailable SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPsrA-R1
Plasmid#74688PurposePsrA-R1 gateDepositorInsertPTac-RBSR1-PsrA-PPsrA-YFP
ExpressionBacterialAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPhlF-P2
Plasmid#74686PurposePhlF-P2 gateDepositorInsertPTac-RBSP2-PhlF-PPhlF-YFP
ExpressionBacterialAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only