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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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32,417 results

Showing: 7161 - 7180 of 32417 results

  1. pExp-mCherry

    Plasmid
    #112569
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and mCherry
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112569-plasmid-map-sequence-id-217213

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  3. SHMT2

    Plasmid
    #25479
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SHMT2 (SHMT2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    A269T
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25479-plasmid-map-sequence-id-168679

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  6. TH

    Plasmid
    #39079
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TH (TH Human)
    Tags
    Tev His Flag
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. BtuF

    Plasmid
    #92209
    Purpose
    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter
    Depositor
    Douglas Rees
    Article
    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99
    Insert
    BtuF (btuF )
    Tags
    6x His Tag and PelB Signal Sequence
    Expression
    Bacterial
    Mutation
    BtuF signal sequence removed and uses the PelB si…
    Promoter
    T7 Promoter
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92209-plasmid-map-sequence-id-183562

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  13. pMM441

    Plasmid
    #61271
    Purpose
    Also referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.
    Depositor
    Timothy Lu
    Article
    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103
    Insert
    Cas9, tracrRNA, crRNA
    Use
    CRISPR
    Tags
    6xHis
    Expression
    Bacterial
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    61271-plasmid-map-sequence-id-99582

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  16. pYFP

    Plasmid
    #175592
    Purpose
    Tet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    mVenus
    Expression
    Bacterial
    Mutation
    An improved monomeric YFP. See doi: 10.1021/bi051…
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    175592-plasmid-map-sequence-id-348320

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Showing: 7161 - 7180 of 32417 results