30,810 results

1. pBS hTSP-4

   Plasmid

   #13001

   Depositor

   Jack Lawler

   Article

   Lawler et al J Biol Chem. 1995 Feb 10. 270(6):2809-14

   Insert

   TSP-4 (THBS4 Human)
   

   Expression

   Bacterial

   Available Since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

   Plasmid

   #85111

   Purpose

   expression of recombinant human mature (delta19) ETFalpha wt without tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta19) ETFalpha (ETFA Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTCF120 H-2D(b)

   Plasmid

   #180454

   Purpose

   E. coli expression of soluble MHC protein

   Depositor

   John Altman

   Insert

   H-2D(b)
   

   Tags

   BSP41

   Expression

   Bacterial

   Mutation

   Replacement of transmembrane domain with enzymati…

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWT004a

   Plasmid

   #107883

   Purpose

   R1 plasmid. Contains PBAD-EGFP and is part of the CAMERA system for rewritable multi-event analog recording in bacteria

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PBAD-EGFP
   

   Expression

   Bacterial

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b_EcSSB_1

   Plasmid

   #78203

   Purpose

   Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

   Depositor

   Martin Webb

   Article

   Dillingham et al Biophys J. 2008 Oct;95(7):3330-9. doi: 1

   Insert

   ssb (G26C)
   

   Expression

   Bacterial

   Mutation

   G26C (please see depositor comment below)

   Promoter

   T7

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p28BIOHTEV-LIC

   Plasmid

   #167852

   Purpose

   Bacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purification

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Tags

   6xHis and AviTag

   Expression

   Bacterial

   Available Since

   April 22, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGBW-m4046757

   Plasmid

   #145657

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTn7xTS-dTomato

   Plasmid

   #117391

   Purpose

   Tn7 tagging vector pTn7xTS with dTomato expression scaffold

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Insert

   dTomato
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

   Plasmid

   #176745

   Purpose

   Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Glt1-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Promoter

   gfaABC1D

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET24a_SMOXdel

    Plasmid

    #186785

    Purpose

    C-terminal His-Tag and a TEV cleaveage site, deletion of 8 residues (503-512) to transform into E. coli BL21 (DE3) cells

    Depositor

    Sandra Gabelli

    Article

    Dunston et al Acta Naturae. 2020 Jul-Sep;12(3):140-144

    Insert

    Spermine oxidase (SMOX Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 503-512

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHelper_TetR/pTet-Tnpase (carbR)

    Plasmid

    #213911

    Purpose

    ATc-inducible himar1C9 transposase expression - carb/amp resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    TetR/ptet ATc-inducible himar1C9 transposase
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDonor_Tn[sfGFP]

    Plasmid

    #213902

    Purpose

    Mariner transposon expressing sfGFP with kan selection casette

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    mariner transposon with kan resistance and superfolder GFP
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGM190oriT

    Plasmid

    #115679

    Purpose

    E. coli-Streptomyces shuttle vector for gene expression

    Depositor

    Günther Muth

    Insert

    oriT, aphII, tsr
    

    Expression

    Bacterial

    Promoter

    tipA

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SND1 (SND1A-c003)

    Plasmid

    #98242

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SND1A (SND1 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00567:TUDOR 677-799

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUK

    Plasmid

    #64185

    Purpose

    harbours marker genes URA3 and LYS2

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Aug. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC-UAS1B8-TEF(136)

    Plasmid

    #44380

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-TEF(136) promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-TEF(136)

    Available Since

    April 17, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pOPINB-hRNF216(511-784)

    Plasmid

    #171923

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWT021a

    Plasmid

    #107895

    Purpose

    W2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    PTetO-SD8-BE2, PLac-sgRNA1
    

    Expression

    Bacterial

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-ATG8(414)/GFP-AUT7(414)

    Plasmid

    #49424

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

    Depositor

    Daniel Klionsky

    Article

    Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Promoter

    ATG8

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pACYCDuet_WTDHFR_WTTS

    Plasmid

    #91226

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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