32,457 results
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Plasmid#124112PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only
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pET21a-TrpRS-His
Plasmid#124120PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-LeuRS-His
Plasmid#124113PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐hsRNASEH2BCA
Plasmid#108692PurposeFor expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzymeDepositorTagsGSTExpressionBacterialMutationShine Dalgarno sequence upstream of start codon a…PromotertacAvailable SinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlyRS-His
Plasmid#124110PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5087
Plasmid#85123PurposePtrc-YFP subcloned from pAM5080 into NSII-Km vector.DepositorInsertYFP
ExpressionBacterialAvailable SinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSG289
Plasmid#115608Purposesurface-displayed Nb2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG291
Plasmid#115605Purposesurface-displayed Null, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG287
Plasmid#115606Purposesurface-displayed Ag2, used in composability experiments; low copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG019
Plasmid#126502PurposePseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHisDepositorInsertdisaggregase ClpG with 6xHis-tag
TagsC-terminal 6xHisExpressionBacterialPromoterPBADAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
Tags14Histidine-TEVExpressionBacterialPromoterT7Available SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
ExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB168
Plasmid#101671PurposeOpto-T7RNAP*(563-F1), equal expr.DepositorInsertOpto-T7RNAP*(563-F1), equal expr.
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIM1708-surT-PsurP-agaN-pNW33N
Plasmid#44009DepositorInsertsurT-PsurP-agaN
ExpressionBacterialMutationDeleted EcoRI site (synonymous change, C to T at …Available SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
HDAC6 (3GV4)
Plasmid#25297PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHDAC6_42 (HDAC6 Human)
TagsHisExpressionBacterialMutationContains amino acid residues 1109-1215Available SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
HS-DN-Cep152-mKate2
Plasmid#105943Purposeheat shock inducible transgenesisDepositorAvailable SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
DT050A
Plasmid#159411PurposeConstitutive murine mature BDNF expression, controlled under E.coli J23101 (strong) promoterDepositorInsertpr.J23101-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
UseSynthetic BiologyTagsFLAGExpressionBacterialPromoterJ23101 promoterAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM596
Plasmid#68885PurposeIPTG-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertsNanoLuc
LacI
ExpressionBacterialAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only