30,735 results

1. pNP1954

   Plasmid

   #222730

   Purpose

   pNP1723 - IS621 WT Donor and bridgeRNA in trans (TBL-T5, DBL-WT)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSIMcpf1

   Plasmid

   #153034

   Purpose

   For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 19 pCOLI_A_SUMO

   Plasmid

   #160257

   Purpose

   N-term 6xHis SUMO 3C cleavable Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   6xHis and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet1_His-TEV-EGFP

   Plasmid

   #223723

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   EGFP, not monomeric (has A207)
   

   Expression

   Bacterial

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAD48-daf-2 RNAi

   Plasmid

   #34834

   Depositor

   Cynthia Kenyon

   Article

   Dillin et al Science. 2002 Oct 25;298(5594):830-4.

   Insert

   daf-2 (daf-2 Nematode)
   

   Use

   RNAi

   Expression

   Bacterial

   Available Since

   June 11, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2

   Plasmid

   #111260

   Purpose

   mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   DsRed-Express2
   

   Expression

   Bacterial

   Promoter

   Pc

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSGKP-km

   Plasmid

   #117233

   Purpose

   A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTlpA39-Wasabi

   Plasmid

   #86116

   Purpose

   mWasabi reporter gene gated by TlpA39

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA39
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A, D135V, A217V, L236F

   Promoter

   pTlpA and pTlpA, LacI

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-28a(+)-FGF2-G3

   Plasmid

   #135521

   Purpose

   Engineered form of fibroblast growth factor 2 with improved thermostability

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   fibroblast growth factor 2 (FGF2 Human)
   

   Tags

   6x His tag and Agg Thrombin Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available Since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBB550

    Plasmid

    #27396

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    dnaK, dnaJ, GroESL
    

    Expression

    Bacterial

    Available Since

    Sept. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC3-tag-C

    Plasmid

    #185481

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/UltralD

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Type

    Empty backbone

    Tags

    StrepIII-HA-UltraID

    Expression

    Bacterial and Mammalian

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTlpA36-Wasabi

    Plasmid

    #86115

    Purpose

    mWasabi reporter gene gated by TlpA36

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA36
    mWasabi
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A, P60L, D135V, K187R, K202I, L208Q

    Promoter

    pTlpA and pTlpA, LacI

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAJE3-E7

    Plasmid

    #214359

    Purpose

    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins

    Depositor

    Ryan Mehl

    Article

    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-

    Inserts

    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    

    Expression

    Bacterial

    Promoter

    AmpR and lpp

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCasKP-apr

    Plasmid

    #117231

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-15b-EK_C122S_His5

    Plasmid

    #49048

    Purpose

    Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

    Depositor

    Hans Brandstetter

    Article

    Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

    Insert

    Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
    

    Tags

    5xHis

    Expression

    Bacterial

    Mutation

    P92R, E185D, C122S

    Promoter

    T7

    Available Since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNCS-mGold2t

    Plasmid

    #231766

    Purpose

    Expression of mGold2t in bacterial cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Expression

    Bacterial

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Synthetic promoter

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNCS-mGold2s

    Plasmid

    #231765

    Purpose

    Expression of mGold2s in bacterial cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s
    

    Expression

    Bacterial

    Mutation

    mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Synthetic promoter

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDonor14-ShHELIX-KanR (CJT70)

    Plasmid

    #181793

    Purpose

    pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Expression

    Bacterial

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJA54 HLA-A*03:01

    Plasmid

    #180451

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*03:01
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET LIC cloning vector (2A-T)

    Plasmid

    #29665

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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