32,838 results
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Plasmid#229066PurposetRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codonDepositorInsertMethanococcus jannaschii tRNA synthetase
ExpressionBacterialPromoterT7Available SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry_Txe/Axe
Plasmid#229147PurposeToxin-Antitoxin (Txe/Axe)DepositorInsertmCherry, Txe/Axe
ExpressionBacterialPromoterPtlpA (mCherry), Txe/Axe (Native)Available SinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28A-Rab8A
Plasmid#228880PurposeExpression of His-tagged Rab8A in E. coliDepositorInsertRAB8A (RAB8A Human)
TagsHis tagExpressionBacterialMutationresidues 1-6 and 177-207 missingAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-Lysozyme fAb
Plasmid#214951PurposeBacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusionsDepositorInsertsVNp-anti-Lysozyme Fab heavy chain fusion
VNp-anti-Lysozyme Fab light chain fusion
TagsVNpExpressionBacterialPromoterT7Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a_LYN
Plasmid#224238PurposeExpression of human LYN Kinase in E. coliDepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaMIgG-14
Plasmid#228994PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-14
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-5
Plasmid#228965PurposeFor bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-5
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-39
Plasmid#228980PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-39
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJCX3-ACTG2_R257C
Plasmid#228911PurposeExpresses mutant R257C smooth muscle actin gamma-2 in human cellsDepositorInsertActin gamma 2, smooth muscle (ACTG2 Human)
TagsHIS-FLAG-TEVExpressionBacterial and MammalianMutationR257CPromoterCMVAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJCX3-ACTG2_WT
Plasmid#228910PurposeExpresses wildtype smooth muscle actin gamma-2 in human cellsDepositorInsertActin gamma 2, smooth muscle (ACTG2 Human)
TagsHIS-FLAG-TEVExpressionBacterial and MammalianMutationWTPromoterCMVAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-Pcym
Plasmid#225280PurposeMini-Tn7 pGP-Tn7-Cm vector with inducible Pcym cumate promoter for the inducible/controlled expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneExpressionBacterialPromoterPcym cumate (p-isopropylbenzoate)-inducible promo…Available SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-Tn7-PcL
Plasmid#225281PurposeMini-Tn7 pGP-Tn7-Cm vector with constitutive PcL promoter for the constitutive expression of target genes inserted into the attTn7 chromosomal site.DepositorTypeEmpty backboneExpressionBacterialPromoterConstitutive PcL promoterAvailable SinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(CysCNP)RS
Plasmid#225054PurposetRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codonDepositorArticleInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDC2
Plasmid#228779PurposeProtein expression plasmid for YTH domain of YTHDC2 in E.Coli.DepositorAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBAD-luxKate
Plasmid#229946PurposeBacterial expression of a de novo designed, FRET-based neoLux1.2-mKate2 fusion bioluminescent probe (max. Em=638)DepositorInsertluxKate
TagsHis-tagExpressionBacterialPromoteraraBADAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJTM-T7-mNeongreen-6xHis
Plasmid#210868PurposePlasmid used for expressing his--tagged mNeongreen for protein purification.DepositorInsertmNeongreen-6xHistag
ExpressionBacterialPromoterT7Available SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/V5-His-mPer1
Plasmid#219421PurposeExpresses 3873 bp of mPer1 coding sequence in E. coli.DepositorAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTlpA_mCherry
Plasmid#229146PurposePromoter strength evaluation of PtlpADepositorInsertmCherry
ExpressionBacterialMutationNoPromoterPtlpAAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7_zntR
Plasmid#216324PurposeT7 promoter driving expression of zntRDepositorInsertZinc-responsive activator
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCN34e-ktrA-yfp
Plasmid#227640Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertktrA-yfp
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-kimA-yfp
Plasmid#227638Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertkimA-yfp
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14
Plasmid#213518PurposeExpresses NSP14 of SARS-CoV-2DepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTYB1-T4uvsX
Plasmid#228213PurposeExpression in BL21(DE3) of T4 recombinaseDepositorInsertuvsX (uvsX Escherichia phage T4)
TagsSce VMA intein-chitin binding domainExpressionBacterialPromoterT7Available SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HaloKbp1b
Plasmid#228794PurposeChemigenetic indicator for potassium ion - low affinityDepositorInsertHaloKbp1b
TagsHis-TagExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(-12)
Plasmid#219420PurposeFluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysateDepositorInsertGFP(-12)
TagsMGHHHHHGGExpressionBacterialPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD289
Plasmid#228113PurposepET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoterDepositorInsertKva1 retron, ncRNA with 90 bp rpoB donor
ExpressionBacterialPromoterT7Available SinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW358f
Plasmid#227935PurposeExpress hIAPP-T7nDepositorInserthIAPP-T7n
ExpressionBacterialAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTW357b
Plasmid#227930PurposeLow-stringency accessory plasmid for selectionDepositorInsertsgIII (recoded)
T7c
ExpressionBacterialMutationR632SAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHelper-CBE-sgRNA
Plasmid#221137PurposeHelper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.DepositorInsertsgRNA-scaffold with Cas9 handle
UseCRISPRExpressionBacterialPromoterJ23119(SpeI)Available SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only