30,844 results

1. pAC-4CL1

   Plasmid

   #35947

   Depositor

   Claudia Schmidt-dannert

   Article

   Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

   Insert

   4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLac-mod

   Available Since

   Sept. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUMV19

   Plasmid

   #67161

   Purpose

   inducible operation of the mevalonate pathway in E. coli

   Depositor

   Tomohisa Kuzuyama

   Article

   Takagi et al J Bacteriol. 2000 Aug;182(15):4153-7.

   Insert

   the mevalonate pathway genes
   

   Expression

   Bacterial

   Promoter

   lacZ

   Available Since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMS29: pDonor(PmcCAST)

   Plasmid

   #168162

   Purpose

   PmcCAST donor harboring non-functional cargo.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST right end (RE)
   PmcCAST left end (LE)
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRU1144

   Plasmid

   #14471

   Depositor

   Philip Poole

   Article

   Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

   Insert

   mRFP1
   

   Expression

   Bacterial

   Available Since

   April 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRE-Tn7-133

   Plasmid

   #118551

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn7 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 5VC9 (CXXC4)

   Plasmid

   #101263

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CXXC4 (CXXC4 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. GST-TPX2(1-43)

   Plasmid

   #69107

   Purpose

   For bacterial expression of human TPX2(1-43aa) tagged with GST.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Targeting Protein for Xklp2 (TPX2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Amino acids 1-43 expressed.

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSN007

   Plasmid

   #102440

   Purpose

   For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

   Depositor

   Alejandro Chavez, George Church

   Article

   Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

   Insert

   7SK paired gRNA insert
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET26_MraYaa

   Plasmid

   #100166

   Purpose

   recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Chung et al Science. 2013 Aug 30;341(6149):1012-1016

   Insert

   mraY (phospho-MurNAc-pentapeptide translocase)
   

   Tags

   MBP-His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. L-GARS-2

    Plasmid

    #166531

    Purpose

    scFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. H2BK120C

    Plasmid

    #75332

    Purpose

    Bacteria expression of H2BK120C

    Depositor

    Cynthia Wolberger

    Article

    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

    Insert

    H2BK120C (h2bc11.S Frog)
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    truncated; K120C

    Promoter

    T7

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAJM.969

    Plasmid

    #108537

    Purpose

    MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    MphRAM + EryR + PMph-YFP
    

    Expression

    Bacterial

    Mutation

    MphR: N94D

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Col1a-3'probe

    Plasmid

    #20731

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    Col1a-3'probe (Col1a1 Mouse)
    

    Expression

    Bacterial

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX4T3-HA-ERK2 R67S

    Plasmid

    #53201

    Purpose

    Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    R67S

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pET11a_PTK2b_Kinase

    Plasmid

    #224242

    Purpose

    Expression of human PTK2b Kinase domain in E. coli

    Depositor

    Andrew Mesecar

    Insert

    ptk2b_kinase (PTK2B Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7 promotor

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. TrpLE-M-nMSP1E3D1

    Plasmid

    #179949

    Purpose

    Tagless membrane scaffold protein (MSP) for formation of nanodiscs.

    Depositor

    Kerney Glover

    Article

    Julien et al Anal Biochem. 2021 Nov 27:114497. doi: 1

    Insert

    TrpLE-M-nMSP1E3D1
    

    Tags

    TrpLE

    Expression

    Bacterial

    Mutation

    Native Trp residues have been mutated to Phe resi…

    Promoter

    T7

    Available Since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLITMUS*-PrpoN-CroAct3 63aa-PBBa_J23106-geneIII

    Plasmid

    #134355

    Purpose

    Phagemid

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    CroAct3 63aa
    

    Expression

    Bacterial

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p404GAL1

    Plasmid

    #17417

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    GAL1 promoter is between SacI and XbaI restrictio…

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pST5832

    Plasmid

    #36256

    Depositor

    Carolyn Bertozzi, Jessica Seeliger

    Article

    Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

    Insert

    lacZ
    

    Use

    Synthetic Biology; Mycobacteria replicating vector

    Expression

    Bacterial

    Promoter

    Modified Hsp60

    Available Since

    Nov. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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