30,176 results

1. pEE060

   Plasmid

   #176817

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   611
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOGG024

   Plasmid

   #113991

   Purpose

   Level 1 golden gate cloning vector for medium copy number free-living expression

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC-CH10-GG

   Plasmid

   #215807

   Purpose

   Cloning of target gene with C terminal non cleavable His10 tag

   Depositor

   Frank von Delft, ASAP Discovery

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pIKM1

   Plasmid

   #51054

   Purpose

   Kanamycin and erethromycin resistance

   Depositor

   Lee Lynd, Juergen Wiegel

   Article

   Tyurin et al Appl Environ Microbiol. 2004 Feb;70(2):8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pSNK

   Plasmid

   #135193

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MDO2-c004

   Plasmid

   #173104

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K7-D243

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMLM36 (aka pBR-UV5-GP-FD2)

   Plasmid

   #21871

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Protoc. 2009. 4(10):1471-501.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBTL-4

   Plasmid

   #22808

   Depositor

   Ryan Gill

   Article

   Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNH-TrxT-G-S18

   Plasmid

   #127827

   Purpose

   Production of recombinant human ribosomal protein S18

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPS18 (RPS18 Human)
   

   Use

   Tags

   His6 - Thioredoxin - TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOT2

    Plasmid

    #14460

    Depositor

    Philip Poole

    Article

    Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

    Insert

    Gfp-UV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAB203

    Plasmid

    #101675

    Purpose

    Opto-T7RNAP(563-F1)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP(563-F1)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHLM

    Plasmid

    #64168

    Purpose

    harbours marker genes HIS3, LEU2 and MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD33MevT

    Plasmid

    #17814

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    MevT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDB070.iodo.5

    Plasmid

    #99397

    Purpose

    Expression plasmid encoding the evolved MjTyrRS variant Iodo.5RS for amber suppression with p-iodophenylalanine

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    PN25 TetR; Ptet Iodo.5RS; PproK tyrT(Opt,CUA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PN25, Ptet, and PproK

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTXB1-TnY

    Plasmid

    #163975

    Purpose

    IPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.

    Depositor

    Peter Smibert

    Article

    Liscovitch-Brauer et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

    Insert

    TnY
    

    Use

    Tags

    Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAN3945

    Plasmid

    #74699

    Purpose

    Circuit plasmid: 0x41

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    Circuit 0x41
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ELF4_pet28a

    Plasmid

    #131661

    Purpose

    Bacterial expression of ETS gene ELF4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF4 (ELF4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LaM8-AK74

    Plasmid

    #159594

    Purpose

    Opto-Nanobody

    Depositor

    Jared Toettcher, Jose Avalos

    Article

    Gil et al Nat Commun. 2020 Aug 13;11(1):4044. doi:

    Insert

    LaM8 with AsLOV2 at AK74
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-SdrG_N2N3-HIS-ybbr

    Plasmid

    #101238

    Purpose

    SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    SdrG_N2N3
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTYB11-PTBP1-RRM2CCtoSS

    Plasmid

    #89154

    Purpose

    recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    Intein and chitin binding domain

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge