30,180 results

1. pBbB9a-GFP

   Plasmid

   #126112

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23113

   Available since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1257 pGEX4T3 GST FKHR

   Plasmid

   #10706

   Depositor

   William Sellers

   Insert

   FKHR (FOXO1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

3. His6tev CBP HAT (1324-1700)

   Plasmid

   #99340

   Purpose

   bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP HAT domain (1324-1700) (Crebbp Mouse)
   

   Use

   Tags

   His6tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD/His-miRFP709

   Plasmid

   #79986

   Purpose

   Monomeric near-infrared fluorescent protein miRFP709 in bacterial plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   miRFP709
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araB

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

   Plasmid

   #110547

   Purpose

   CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.

   Depositor

   Ichiro Matsumura

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pBAD

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEW3: pET-32a Dot1L(2-416)

   Plasmid

   #124098

   Purpose

   Dot1L(2-416) expression plasmid

   Depositor

   Cynthia Wolberger

   Article

   Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

   Insert

   Dot1L(2-416) (DOT1L Human)
   

   Use

   Tags

   Trx-His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC-HIS6-GST-TEV-GG

   Plasmid

   #215801

   Purpose

   Cloning of target gene with N terminal His6 GST tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Caspase-6

   Plasmid

   #100102

   Purpose

   Bacterial expression of Caspase-6

   Depositor

   Jim Wells

   Article

   Seaman et al Cell Death Differ. 2016 Oct;23(10):1717-

   Insert

   Caspase-6 (CASP6 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR1-kan-Ptet-plu-gamma-ETh_rhi483

   Plasmid

   #180429

   Purpose

   Genome engineering of Agrobacterium

   Depositor

   Jun Fu

   Article

   Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

   Insert

   plu-gamma-ETh_rhi483
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMVV098

    Plasmid

    #215304

    Purpose

    Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    PPhIF

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC029 - Lw2Cas13a from Leptotrichia wadei F0279

    Plasmid

    #91919

    Purpose

    Expresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    Lw2Cas13a and guide expression cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG1b_10xHisTEVATG5_ATG12

    Plasmid

    #190861

    Purpose

    Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG5 (ATG5 Human)
    ATG12 (ATG12 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ReactionC_pBIG1a_ATG7_ATG10

    Plasmid

    #190860

    Purpose

    Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG7 (ATG7 Human)
    ATG10 (ATG10 )
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CECR2

    Plasmid

    #39066

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CECR2 (CECR2 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. INT_Cargo_PgyrA_gfp

    Plasmid

    #212138

    Purpose

    Cargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.

    Depositor

    Kang Zhou

    Insert

    green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDSG289

    Plasmid

    #115608

    Purpose

    surface-displayed Nb2, used in composability experiments; low copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQacR-Q2

    Plasmid

    #74690

    Purpose

    QacR-Q2 gate

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    PTac-RBSQ2-QacR-PQacR-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. DOT1L

    Plasmid

    #36196

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (GeneScript Synthesized) (DOT1L Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-420

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. 6ASB (FBXL19)

    Plasmid

    #119954

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    FBXL19 (FBXL19 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 31-153

    Promoter

    T7

    Available since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pTriEx-NTOM20-mVenus-Zdk3

    Plasmid

    #81012

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk3
    

    Use

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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