30,875 results

1. pBAD-HisD-KRaION2

   Plasmid

   #177800

   Purpose

   Bacterial expression of green fluorescent potassium indicator KRaION2

   Depositor

   Edward Boyden

   Article

   Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

   Insert

   KRaION2
   

   Expression

   Bacterial

   Promoter

   araB

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME GFP-PCNA

   Plasmid

   #105977

   Purpose

   entry clone for gateway cloning, cell cycle marker proliferative cell nuclear antigen PCNA

   Depositor

   Caren Norden

   Article

   Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

   Insert

   PCNA (PCNA Human)
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-Rsn2

   Plasmid

   #61686

   Purpose

   bacterial expression of ranaspumin 2 as a His-tagged fusion protein

   Depositor

   Brian Smith

   Article

   Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

   Insert

   Ranaspumin-2
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLM36 (aka pBR-UV5-GP-FD2)

   Plasmid

   #21871

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Protoc. 2009. 4(10):1471-501.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBP-C_ccdB

   Plasmid

   #204114

   Purpose

   Type 0 CDS part

   Depositor

   Mikhail Shapiro

   Insert

   Toxic DNA gyrase inhibitor ccdB
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSR357.24

   Plasmid

   #125109

   Purpose

   Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   narL(REC)-ydfI(DBD)159
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. YopH-pNIC-Bsa4

   Plasmid

   #92063

   Purpose

   pNIC expression vector encoding gene for YopH

   Depositor

   Josh Beckham

   Insert

   YopH
   

   Tags

   6x His Tag

   Expression

   Bacterial

   Mutation

   Codon optimized E. coli

   Available Since

   Jan. 17, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pQE-30-RexYFP

   Plasmid

   #141070

   Purpose

   Bacterial expression of genetically encoded fluorescent indicator for measuring NAD+/NADH ratio

   Depositor

   Vsevolod Belousov

   Article

   Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

   Insert

   RexYFP
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed Leucine 169 to Proline, changed Tyrosine …

   Promoter

   T5

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEB2-2ndVal mCherry2-L

   Plasmid

   #104027

   Purpose

   Plasmid encoding 2ndVal mCherry2-L

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   2ndVal mCherry2-L
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   mCherry2-L with valine inserted in 2nd position

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM586

    Plasmid

    #19978

    Depositor

    David Waugh

    Article

    Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

    Insert

    TEV protease (TEVgp1 tobacco etch virus)
    

    Expression

    Bacterial

    Mutation

    S219D mutant

    Available Since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTet-tns

    Plasmid

    #170628

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTnsABC
    

    Expression

    Bacterial

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIC-Cre

    Plasmid

    #19131

    Depositor

    Klaus Rajewsky

    Article

    Gu et al Cell. 1993 Jun 18. 73(6):1155-64.

    Insert

    Cre recombinase (cre Bacteriophage P1)
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pVRb20_992

    Plasmid

    #49714

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    sfgfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Pecf20_992

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-PP7v3

    Plasmid

    #107230

    Purpose

    Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    24xPP7v3
    

    Tags

    3x FLAG-tag and P2A site

    Expression

    Bacterial

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFTP1

    Plasmid

    #64952

    Purpose

    source of TpR FRT cassette

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    FRT-dhfrII-FRT
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET3d-E2-25K-C170S

    Plasmid

    #18892

    Depositor

    Cecile Pickart

    Article

    Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

    Insert

    Ubiquitin conjugating enzyme E2-25K (UBE2K Human, Most likely H. sapiens)
    

    Expression

    Bacterial

    Mutation

    Cysteine 170 is replaced with a Serine residue.

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

17. B1-5

    Plasmid

    #111467

    Purpose

    translational elongation factor Ts

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    translational elongation factor Ts
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pE5c

    Plasmid

    #17465

    Purpose

    modified pENTR vector for C-terminal ECFP tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    ECFP

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTD-C_eYFPTwinStrep

    Plasmid

    #45942

    Purpose

    expresses E.coli codon optimized EYFP-Twin-Strep fusion protein

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic eYFP (codon usage adapted to E.coli K12)
    

    Tags

    Twin-Strep-tag

    Expression

    Bacterial

    Promoter

    lacIq/Ptrc

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. cr3Ec

    Plasmid

    #184860

    Purpose

    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    Cas9, gam, bet, exo
    

    Tags

    Cas9 ssrA degradation tag

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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