30,875 results

1. pTA-attB

   Plasmid

   #18937

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   phiC31 attB
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEND-303_Cacnes_tetR

   Plasmid

   #225618

   Purpose

   C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   TetR
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEND-304_Cacnes_tetR

   Plasmid

   #225619

   Purpose

   C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO1O3)+RBS_1+mCherry

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   TetR
   

   Use

   Synthetic Biology

   Tags

   6xHis and mCherry-V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEND-375_Cacnes_phlF

   Plasmid

   #225620

   Purpose

   C. acnes with PhlF-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+PhlF // P(BBa_23119-PhlO1O3)+RBS_1+mCherry.

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   PhlF
   

   Use

   Synthetic Biology

   Tags

   6xHis and mCherry-V5-6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEND-192_Cacnes_HS

   Plasmid

   #225621

   Purpose

   C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   HspR
   

   Use

   Synthetic Biology

   Tags

   pFAST-6XHis

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEND-226_Cacnes_mCitrine

   Plasmid

   #225613

   Purpose

   Replicative vector for green fluorescent reporter protein mCitrine strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCitrine

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   mCitrine
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIS002

   Plasmid

   #194013

   Purpose

   This plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.

   Depositor

   Timothy Whitehead

   Article

   Strawn et al Biotechnol Bioeng. 2023 Oct;120(10):3057

   Insert

   C16 Ex3 XTR f1
   

   Expression

   Bacterial

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEB1-mCerulean3

   Plasmid

   #103973

   Purpose

   Plasmid encoding mCerulean3

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mCerulean3
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F64L, Y66W, …

   Promoter

   proC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXWJ109Hg-gfp

   Plasmid

   #123148

   Purpose

   A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-32merR-t-PmerT-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J109, PmerT

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSETb-ffDronpa

    Plasmid

    #78182

    Purpose

    Expresses ffDronpa E. coli strains

    Depositor

    Peter Dedecker

    Article

    Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

    Insert

    ffDronpa
    

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4092677

    Plasmid

    #153883

    Purpose

    Bacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stop

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    fragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    wt;handful of SNPs as watermarks

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pC009 LshCas13a locus with targeting spacer

    Plasmid

    #91900

    Purpose

    LshCas13a locus into pACYC184 with targeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with targeting spacer
    

    Expression

    Bacterial

    Available Since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pTE101

    Plasmid

    #59396

    Purpose

    MethyloBrick cloning vector, no promoter; KmR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    none

    Available Since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOGG026

    Plasmid

    #113992

    Purpose

    Level 1 golden gate cloning vector for low copy number stable environmental gene expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLlR5

    Plasmid

    #149464

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    LacI-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLlacO-1

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJF076Sa

    Plasmid

    #113322

    Purpose

    J1-mRFP1

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    mRFP1
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. HS63 pComb3xss anti-Homer1 nanobody

    Plasmid

    #134715

    Purpose

    Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))
    

    Tags

    6xHis-HA

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET19b+H2B

    Plasmid

    #157809

    Purpose

    Expression of human histone H2B

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    H2B
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQTEV-PPP1R1A

    Plasmid

    #31331

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    protein phosphatase 1 (PPP1R1AP1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. His-DERA

    Plasmid

    #203225

    Purpose

    For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    deoxyribose aldolase
    

    Tags

    N-terminal TEV cleavable 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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