33,128 results
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Plasmid#84579PurposeShuttle and expression vector. Predicted to replicate in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Tetracycline-inducible expression of cloned genes.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 21, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits
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pAB045a
Plasmid#79224PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ rpoZ-HA4; PlacIq lacI
ExpressionBacterialPromoterPlacZ; PlacIqAvailable SinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30-roGFP1-iL
Plasmid#83313PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-iL
Tags6xHISExpressionBacterialMutationro1(C48S/S147C/Q204C) plus X insertion, iL betwe…PromoterT5Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-RanGAP
Plasmid#79881PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
TagsAviExpressionBacterialPromoterUbiquitin promoterAvailable SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECJ3D
Plasmid#75467PurposeToggle switch plasmid containing lacI-pdt3DDepositorInsertlacI-pdt3D
ExpressionBacterialPromoterpLtetOAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECT5
Plasmid#75451PurposePlasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicateDepositorInsertpdt5
ExpressionBacterialPromoternoneAvailable SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEM-WingGFP-tan
Plasmid#83473PurposeEGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.DepositorInsertWingGFP-tan
UseCRISPRExpressionBacterial and InsectPromoterHsp70 and enhancer from D. melanogaster yellow ge…Available SinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
R10-3 /pQE-30
Plasmid#79859PurposeThe first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.DepositorInsertR10-3
TagsHisTagExpressionBacterialMutationAequorea victoria green fluorescent protein (avGF…PromoterT5 promoter / lac operatorAvailable SinceSept. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH
Plasmid#64178Purposeharbours marker gene HIS3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHUM
Plasmid#64170Purposeharbours marker genes HIS3, URA3 and MET17DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV114
Plasmid#63181PurposepRS414 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 V416T
Plasmid#81040Purposemammalian expression of LOV V416TDepositorInsertLOV2 V416T
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416TAvailable SinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTL032
Plasmid#80928Purpose14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxPDepositorInsert14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP
ExpressionBacterialAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDZ585 pKAN 2x-mCherry
Plasmid#72236PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherryDepositorInsert2x-mCherry
ExpressionBacterialAvailable SinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Eptesicus fuscus
Plasmid#78113PurposeNt His-tag Eptesicus fuscus Sacsin 89-1368DepositorInsertSacsin Eptesicus fuscus 89-1368
TagsHis-TagExpressionBacterialAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
HpMscS
Plasmid#78310PurposeH. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG TagDepositorInsertMechanosensitive channel of small conductance
Tags6x His Tag and FLAG TagExpressionBacterialPromoterT7 PromoterAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
CBS
Plasmid#73238PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK667
Plasmid#71690PurposeProduces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)DepositorAvailable SinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLmrA-N1
Plasmid#74684PurposeLmrA-N1 gateDepositorInsertPTac-RBSN1-LmrA-PLmrA-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJM105: pTac RFP on pCM66T
Plasmid#74746PurposepCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)DepositorInsertRFP (BioBrick part number BBa_E1010)
UseSynthetic BiologyExpressionBacterialPromoterpTacAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only