30,875 results

1. pAAP2

   Plasmid

   #171208

   Purpose

   bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease

   Depositor

   Geraldine Seydoux

   Article

   Schmidt et al bioRxiv 2020.10.15.340570

   Insert

   pgl-3 (pgl-3 Nematode)
   

   Tags

   MBP::HIS::TEV cleavage site

   Expression

   Bacterial

   Available Since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiex-1 Myosin VI HMM_SNAP

   Plasmid

   #82716

   Purpose

   Expresses Myosin VI HMM with a C-terminal SNAP tag.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Hariadi et al Nat Nanotechnol. 2015 Aug;10(8):696-700.

   Insert

   Myosin VI
   

   Tags

   6xHis Tag, FLAG purification tag, and SNAP tag

   Expression

   Bacterial and Insect

   Promoter

   T7 Promoter

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-UAP56

   Plasmid

   #157661

   Purpose

   Expresses UAP56 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Spliceosome RNA helicase DDX39B (DDX39B Human)
   

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-43

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFPV25

   Plasmid

   #20667

   Depositor

   Raphael Valdivia

   Article

   Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

   Type

   Empty backbone

   Tags

   GFP mut3

   Expression

   Bacterial

   Available Since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRBBm59

   Plasmid

   #48115

   Purpose

   Shuttle vector E. coli/B.meg.

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

   Insert

   promoter sacB - gfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. GLI-3 bs-2

   Plasmid

   #16420

   Depositor

   Bert Vogelstein

   Article

   Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

   Insert

   GLI3 (GLI3 Human)
   

   Expression

   Bacterial

   Available Since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

7. BASIC_3_Kan

   Plasmid

   #68137

   Purpose

   storage vector for BASIC part: Kan

   Depositor

   Geoffrey Baldwin

   Article

   Storch et al ACS Synth Biol. 2015 Apr 16.

   Insert

   Kanamycin
   

   Expression

   Bacterial

   Available Since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6T7G

   Plasmid

   #71463

   Purpose

   Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

   Depositor

   Willie Mark

   Article

   Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   human U6/phage T7

   Available Since

   Feb. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBV514

   Plasmid

   #204602

   Purpose

   Expresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoter

   Depositor

   Scott Moye-Rowley

   Insert

   Os Tir1 (LOC4335696 Oryza sativa)
   

   Tags

   Myc epitope tags

   Expression

   Bacterial

   Mutation

   Changed phenylalanine 74 into an alanine (F74A).

   Promoter

   ScADH1

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. VV020: WT Arch-3 - eGFP in pET28b

    Plasmid

    #58488

    Purpose

    Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

    Depositor

    Adam Cohen

    Article

    Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Archaerhodopsin-3
    

    Tags

    eGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS1950

    Plasmid

    #205983

    Purpose

    Ga0116245_10002768 T7/LacO-Cas6 + TwinStrep-Cas14 + Cas7 + His14-Cas5::pJ23119-DR-Fn spacer-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Inserts

    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    CRISPR array
    

    Tags

    14xHis and TwinStrep-tag

    Expression

    Bacterial

    Promoter

    T7 (proteins), pJ23119 (crRNA)

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG308-His-redFAST

    Plasmid

    #160350

    Purpose

    Expresses His-tagged redFAST in bacteria

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    His-redFAST
    

    Expression

    Bacterial

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYX-N-Puro-mNeon-WNK3

    Plasmid

    #222674

    Purpose

    endogenously tag WNK3 with N-terminal mNeon

    Depositor

    Jason Moffat

    Article

    Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

    Insert

    WNK3 (WNK3 Human)
    

    Tags

    mNeon

    Expression

    Bacterial

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKRR5

    Plasmid

    #149468

    Purpose

    Broad-Host range expression vector, RK2 and pUC origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    RhaS-RhaR-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PrhaBAD

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWJ1

    Plasmid

    #90484

    Purpose

    For gene disruption in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

    Depositor

    Sheng Yang

    Article

    Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

    Insert

    pIM13 Origin
    

    Expression

    Bacterial

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKI1

    Plasmid

    #199118

    Purpose

    Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    H6-SNAP-RapA
    

    Tags

    6xHis and SNAP-tag

    Expression

    Bacterial

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-3-SH3BP1

    Plasmid

    #136647

    Purpose

    Expression of SHBP1-GST fusion protein in E. coli

    Depositor

    Maria Balda, Karl Matter

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1 (SH3BP1 Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Gi-c001

    Plasmid

    #173078

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST17-TARG1

    Plasmid

    #172594

    Purpose

    For purification (from E.coli) of His-tagged TARG1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    TARG1 (OARD1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2T-CAG-KKHSaCas9-BlastR

    Plasmid

    #107189

    Purpose

    Confers constitutive expression of KKH SaCas9.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Insert

    KKH SaCas9 (NEWENTRY )
    

    Expression

    Bacterial

    Mutation

    E782K/N968K/R1015H

    Promoter

    Chicken ß-Actin

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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