31,182 results
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Plasmid#162273PurposeBacterial ExpressionDepositorAvailable SinceDec. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits
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CBX7
Plasmid#25241PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTD-C_sfYFPTwinStrep
Plasmid#45943Purposeexpresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion proteinDepositorInsertsynthetic sfYFP (codon usage adapted to P.putida KT2440)
TagsTwin-Strep-tagExpressionBacterialPromoterlacIq/PtrcAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-G93A-N’His
Plasmid#117705Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
TagsHis tag with Thrombin cleavage siteExpressionBacterialMutationG93APromoterT7Available SinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-2xEcMTS
Plasmid#133624PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherry-2xEcMTS
TagsHisExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
PP-GSK3
Plasmid#25133PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertcgd4_240:C29838
TagsHisExpressionBacterialAvailable SinceAug. 5, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCRT7 NT Topo arabinose
Plasmid#73446PurposeUsed for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptidesDepositorTypeEmpty backboneTagsGST (N-terminal), 6xHis (C-terminal)ExpressionBacterialPromoterpBadAvailable SinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA
Plasmid#99875PurposeExpression of GFP-AURKA for protein purification in bacteriaDepositorAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTSlb-N748D
Plasmid#60732PurposeExpresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.DepositorInsertsResidues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationResidues 1-180 of split T7 RNAP and Residues 180-…Available SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP12049
Plasmid#222355PurposeTn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.DepositorInsertsfGFP
ExpressionBacterialPromoterPabstBRAvailable SinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-TEV-Nsp15
Plasmid#187656PurposeE. coli plasmid (T7lac promoter) for full-length, wild-type SARS CoV-2 Nsp15 (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tagDepositorInsertNsp15 (N Severe acute respiratory syndrome coronavirus 2)
TagsTEV protease cleavable His6-tagExpressionBacterialMutationnonePromoterT7lacAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZmUbi-5'UTR
Plasmid#154057PurposeLevel 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)DepositorInsertPromoter and 5' UTR Ubiquitin (Zea mays)
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH3U3-Zif268
Plasmid#12610DepositorInsertZif268 binding site in pH3U3
ExpressionBacterialAvailable SinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRKO
Plasmid#49456PurposeloxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptionsDepositorInsertloxP-kanMX-loxP cassette
UseCre/LoxExpressionBacterialPromoternoneAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
ExpressionBacterialPromoterVhbAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His
Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
TagsHA-6HIS and MKIEEExpressionBacterialAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28 E2 LBD-Tev-E1a-His
Plasmid#232117PurposeFor bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.DepositorInsertDBT (DBT Human)
Tags6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…ExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 Flag-LplA
Plasmid#232118PurposeFor bacterial expression of Flag-tagged E. coli LplA.DepositorInsertlplA (lplA Escherichia coli (strain K12))
TagsFlagExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28 His-MBP-TEV-BAP-Human BCKDK
Plasmid#232116PurposeFor bacterial expression of Human BCKDK (AA31–412, missing precursor peptide) with an N-terminal biotin acceptor peptide and a cleavable His-MBP purification tag.DepositorInsertBCKDK (BCKDK Human)
Tags6 x His, Biotin acceptor peptide (BAP), Maltose-B…ExpressionBacterialPromoterT7 PromoterAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW357bN
Plasmid#227932PurposeAccessory plasmid for negative selectionDepositorInsertsgIII-neg
T7c
ExpressionBacterialMutationR632SAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only