34,238 results
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Plasmid#87055PurposeExpresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORFDepositorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only
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pSHS293
Plasmid#87348PurposeD435C/E945C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, lobe closure FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/D435C/C574S/E945CPromoterT7Available sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBad-HisB-GGvT
Plasmid#87362PurposeExpresses GGvT, a tandem homodimer green fluorescent proteinDepositorInsertGGvT
TagsHis-TagExpressionBacterialAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-irp3 WT
Plasmid#87106Purposeexpresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocoliticaDepositorInsertirp3 WT
Tagshis-tagExpressionBacterialPromoterT7Available sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRpoH-Wasabi
Plasmid#86107PurposemWasabi reporter gene driven by RpoH promoterDepositorInsertmWasabi
Tags6xHisExpressionBacterialPromoterpRpoHAvailable sinceFeb. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-Ets1-ETS
Plasmid#85735PurposeExpresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440DepositorInsertDNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Mouse, Human)
Tags6xHis following thrombin cleavage site LVPR|GExpressionBacterialAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFA6a-PAmCherry-KanMX6
Plasmid#87026PurposeTag S. pombe genes in C terminal with PAmCherryDepositorInsertPAmCherry
ExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-18
Plasmid#79933PurposePH-4GS-I3-01-myc-Late1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEPN-18
ExpressionBacterial and MammalianAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ oDam_f_GFPoNLS
Plasmid#85819PurposeiDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertoDam_f_GFPoNLS
TagsoNLS (optimized nuclear localization signal) C te…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…Available sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQhsCDtRNA+
Plasmid#86014PurposeMulti-gene expression system for purification of human box C/D RNPs with U14-tRNA minigeneDepositorInsertsUseCo-expression; purificationTags6x-HisExpressionBacterialMutationamino acids 1-401, amino acids 1-411, and amino a…PromoterLPP and PtacAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-SUMO-KaiA
Plasmid#68049PurposeStrain: AM5115, Used for protein purificationDepositorInsertKaiA
Tags6xHis and SUMOExpressionBacterialPromoterT7Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB076i7
Plasmid#79201PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-SH2ABL1
ExpressionBacterialPromoterPlacZ-opt (OR1); Ppro1Available sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
NEK7_HUMAN_D0
Plasmid#79735PurposeThis plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
MARK3_HUMAN_D0
Plasmid#79740PurposeThis plasmid encodes the kinase domain of MARK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VGFR1_HUMAN_D0
Plasmid#79743PurposeThis plasmid encodes the kinase domain of VGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertVGFR1
TagsHis10-TEVExpressionBacterialPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
FAK2_HUMAN_D0
Plasmid#79747PurposeThis plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
CHK2_HUMAN_D0
Plasmid#79715PurposeThis plasmid encodes the kinase domain of CHK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB1_HUMAN_D0
Plasmid#79694PurposeThis plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MCV
Plasmid#81174PurposeMCS2 Cloning Vector - Plasmid for Single Integration Vector MSC2 cloning of insertsDepositorTypeEmpty backboneExpressionBacterialAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUS232
Plasmid#84579PurposeShuttle and expression vector. Predicted to replicate in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Tetracycline-inducible expression of cloned genes.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceOct. 21, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits