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Bacterial Expression

30,735 results

Showing: 801 - 820 of 30735 results

  3. pCJ136

    Plasmid
    #162665
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162665-plasmid-map-sequence-id-321451

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  6. pUC57-TPNOX

    Plasmid
    #87853
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    TPNOX
    Tags
    C-terminal FLAG tag with a linker
    Expression
    Bacterial
    Available Since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87853-plasmid-map-sequence-id-175126

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  8. eSrtA(2A-9) pET29b

    Plasmid
    #75145
    Purpose
    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity
    Depositor
    David Liu
    Article
    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11
    Insert
    reprogrammed sortase A variant
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S102C A104H E105D K138P K152I N160K K162H T164N K…
    Promoter
    T7
    Available Since
    July 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    75145-plasmid-map-sequence-id-466935

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  9. pGBdest-GST-PI3Kc1

    Plasmid
    #187992
    Purpose
    Plasmid for the protein expression of GST-3C-ATG14/Vps34/Vps15/BECN1 in a bacterial and insect expression system. Internal_Reference: SMC1326
    Depositor
    Sascha Martens
    Inserts
    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    Tags
    GST
    Expression
    Bacterial and Insect
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    187992-plasmid-map-sequence-id-374167

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  14. pZP06C

    Plasmid
    #232322
    Purpose
    pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.
    Depositor
    Chenggang Wu
    Insert
    mCherry
    Expression
    Bacterial
    Promoter
    rpsJ
    Available Since
    March 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    232322-plasmid-map-sequence-id-462278

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  17. pBBR15.2-2H12.D11opt-scarRef

    Plasmid
    #221150
    Purpose
    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen2.1
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen2.1)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221150-plasmid-map-sequence-id-437166

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Showing: 801 - 820 of 30735 results