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32,126 results

Showing: 8241 - 8260 of 32126 results

  3. AWP-031

    Plasmid
    #213967
    Purpose
    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.
    Depositor
    Alexander Westermann
    Article
    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121
    Insert
    Cas12a (cas12a Synthetic, Segatella bryantii)
    Tags
    3XFLAG
    Expression
    Bacterial
    Mutation
    D875A to inactivate target nuclease activity
    Promoter
    PcfxA, IPTG inducible
    Available Since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213967-plasmid-map-sequence-id-429166

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  6. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid
    #192349
    Purpose
    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    Tags
    6X-His-Sumo
    Expression
    Bacterial
    Mutation
    Cysteine 145 of the active site is substituted wi…
    Promoter
    T7 lac
    Available Since
    June 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    192349-plasmid-map-sequence-id-381344

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  10. pGEX6P1 METTL3

    Plasmid
    #200731
    Purpose
    Bacterial expression of GST-Tagged codon optimized human METTL3
    Depositor
    Claudio Alarcón
    Insert
    METTL3 (METTL3 Human)
    Tags
    GST from backbone plasmid
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200731-plasmid-map-sequence-id-401808

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  11. pGEX-2T-Cdc42-T17N

    Plasmid
    #12970
    Depositor
    Gary Bokoch
    Insert
    Cdc42 DN (CDC42 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    T17N Dominant negative
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  13. pMCSG53_Nsp9

    Plasmid
    #167264
    Purpose
    Bacterial expression of Nsp9
    Depositor
    Alexei Savchenko
    Insert
    Nsp9 (residues 1-113) (ORF1ab SARS-CoV-2)
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Available Since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167264-plasmid-map-sequence-id-331338

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  14. EHMT1 (2IGQ)

    Plasmid
    #25504
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EHMT1 (EHMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C-terminal domain, residues 951-1235
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pJYP1

    Plasmid
    #202406
    Purpose
    Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)
    Depositor
    Remy Loris
    Article
    Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    fdeA
    Available Since
    Aug. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202406-plasmid-map-sequence-id-403395

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  20. p5059

    Plasmid
    #198154
    Purpose
    bacterial expression of steC::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsteC steC::L16::HaloTag::HA
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    198154-plasmid-map-sequence-id-386983

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Showing: 8241 - 8260 of 32126 results