32,671 results
-
Plasmid#40108DepositorInsertBistable switch using dynamic degradation
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
T4 Lysozyme L99A WT*
Plasmid#18476DepositorAvailable SinceAug. 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCPP5910
Plasmid#128717PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcO-hopO1-1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5058
Plasmid#128710PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcF-hopF2
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-H391Y
Plasmid#122692PurposeExpress His-tagged PKM2 (H391Y) protein in bacteria.DepositorAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-21a_2xNLS_LbCpf1_protein_expression
Plasmid#114366PurposeProtein expression plasmid for 2xNLS LbCpf1 in pET-21a backboneDepositorInsert2xNLS_LbCpf1
UseCRISPRTagsNLSExpressionBacterialPromoterT7Available SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcold-6xHis-HRV3Csite-HsTRBP
Plasmid#41096DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCAH01Sp::Cas9D10A
Plasmid#128161PurposePtet-controlled expression of S. pyogenes Cas9D10A variantDepositorInsertSp Cas9
ExpressionBacterialMutationD10APromoterPtetAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
His_Naa80d in pTYB12
Plasmid#188455PurposeExpress in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.DepositorAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 vatD
Plasmid#112885PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertvatD
ExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72113-BBa_J72152
Plasmid#40784DepositorInsertPhagemid + GFP
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFPV25
Plasmid#20667DepositorTypeEmpty backboneTagsGFP mut3ExpressionBacterialAvailable SinceMarch 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
MRAS
Plasmid#55661Purposeexpression of mouse MRASDepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2
Plasmid#183741PurposeExpresses Ki67 (8 repeats+LR)-mNeonGreenDepositorInsertMKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianMutationMKI67 LR domain + 8 Ki-67 repeatsPromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPS0254
Plasmid#115506PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celBDepositorInsertRlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
DDX20A
Plasmid#42376PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HaloTag7SS-DogTag
Plasmid#171775PurposeExpresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formationDepositorInsertHaloTag7SS-DogTag
TagsHis6ExpressionBacterialMutationDogTag inserted in HaloTag7 between residues D139…PromoterT7Available SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Poliovirus 1 PV1 isolate 09_05_19 3C protease
Plasmid#228647PurposeBacterial expression of the Polio 3C proteaseDepositorInsertPolio virus 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKJ0464
Plasmid#228716PurposeBacterial expression of T7 RNAP with E643G mutation; for protein production and purificationDepositorArticleInsertSUmo_T7pol_E643G
ExpressionBacterialMutationE643GAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJH628_pJH_LldR_PlldR-sfGFP.3TAG
Plasmid#163727PurposeLactate-inducible expression of green fluorescent protein encoding three amber codonsDepositorInsertlldr gfp.3TAG
ExpressionBacterialMutationgfp.N39TAG,N135TAG,Y151TAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only