30,875 results

1. pR1G108CP20X

   Plasmid

   #85086

   Purpose

   Expresses PCNA1 variant (G108C) fused to putidaredoxin reductase and putidaredoxin in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The G108C variant of PCNA1 fused to putidaredoxin reductase and putidaredoxin
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   G108C in PCNA1

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. 28 pCOLI_S_STREP

   Plasmid

   #160262

   Purpose

   N-term 2xStrepII 3C cleavable Spec resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   2xStrepII

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEB2-Katushka

   Plasmid

   #104008

   Purpose

   Plasmid encoding Katushka

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   Katushka
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (E3V, K6T, N21D, R3…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET3a-His-DJ1

   Plasmid

   #51488

   Purpose

   Inducible expression of human His-DJ1 in E.coli

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   DJ1 (PARK7 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 31, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. ARH3-c002

   Plasmid

   #173095

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   ADPRS (ADPRS Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   A14-S363

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGV3341

   Plasmid

   #170279

   Purpose

   Expression of mutant HIV-2 RT p66 reverse transcriptase

   Depositor

   Gregory Van Duyne

   Article

   Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

   Insert

   HIV-2 RT p66 (mutant)
   

   Expression

   Bacterial

   Promoter

   T7-lac

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHelper-T7IS1

   Plasmid

   #140631

   Purpose

   Expresses ShCAST under the T7 promoter and an empty sgRNA

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHis6-SUMO-g0s2

   Plasmid

   #179293

   Purpose

   E. coli expression plasmid (T7 promoter) for full-length human G0S2 with N-terminal His6 + SUMO + TEV protease cleavage site

   Depositor

   Debra Hansen

   Article

   Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do

   Insert

   G0S2 (G0S2 Human)
   

   Tags

   His6 + SUMO + TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   full-length G0S2, residues 1-103 from accession n…

   Promoter

   T7

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Tbr1

   Plasmid

   #15543

   Depositor

   John Rubenstein

   Article

   Bulfone et al Neuron. 1995 Jul . 15(1):63-78.

   Insert

   Tbr1 (Tbr1 Mouse)
   

   Expression

   Bacterial

   Available Since

   Aug. 20, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pJTM-T7-mNeongreen-6xHis

    Plasmid

    #210868

    Purpose

    Plasmid used for expressing his--tagged mNeongreen for protein purification.

    Depositor

    Richard Murray

    Article

    Meyerowitz et al ACS Synth Biol. 2024 Feb 16;13(2):530-53

    Insert

    mNeongreen-6xHistag
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

    Plasmid

    #169160

    Purpose

    To express nsp14-nsp10 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-R-GECO1.2

    Plasmid

    #113937

    Purpose

    Bacterial expression of red fluorescent Ca2+ sensor R-GECO1.2

    Depositor

    Robert Campbell, Thomas Hughes

    Article

    Molina et al Biophys J. 2019 May 21;116(10):1873-1886

    Insert

    R-GECO1.2
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBFC0687

    Plasmid

    #186692

    Purpose

    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    

    Expression

    Bacterial

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKIKan_idCas9_Chr_center

    Plasmid

    #149637

    Purpose

    suicide vector for knock in into the B. burgdorferi chromosome

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-SUMO- flag-hDDI2-D252N_pET11a

    Plasmid

    #169123

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Mutation

    D252N

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. His-SUMO-flag-hDDI2_pET11a

    Plasmid

    #169121

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTA-attP

    Plasmid

    #18939

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    phiC31 attP
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQTEV-PDAP1

    Plasmid

    #31586

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PDGFA associated protein 1 (PDAP1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. H11-CAG-loxP-FNF-bpA-3pA-loxP-tdTomato

    Plasmid

    #180156

    Purpose

    tdTomato cre reporter with Hipp11 recombineering homology arms

    Depositor

    Mario Capecchi

    Insert

    tdTomato
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Bacterial and Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. B3-1

    Plasmid

    #111471

    Purpose

    translational initiation factor 2

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    translational initiation factor 2
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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