33,526 results

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pCC28

Plasmid

#128284

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Publication

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, sopC

Expression

Bacterial

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAA3

Plasmid

#128280

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Publication

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, parS

Expression

Bacterial

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pOSV803

Plasmid

#126596

Purpose

modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

Depositor

Jean-Luc Pernodet

Publication

Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-C9ORF80

Plasmid

#128418

Purpose

Express C9ORF80 in E. coli with a His tag (C-terminal)

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

C9ORF80 (INIP Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCAM17

Plasmid

#86473

Purpose

Expresses p12 and p9

Depositor

Pamela Silver

Publication

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p12, p9

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP22x-1A(sfGFP)

Plasmid

#126969

Purpose

Level 1 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pES134

Plasmid

#127551

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Publication

Wu et al Microbiology (Reading). 2024 Mar;170(3).

Insert

F3-flanked spectinomycin resistance cassette

Use

Synthetic Biology

Expression

Bacterial

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD3-Lux-nat

Plasmid

#120323

Purpose

Bacterial luminescence promoter trap

Depositor

James Kirby

Publication

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Expression

Bacterial

Promoter

(None)

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-7D12Gln13TAG

Plasmid

#125264

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Publication

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

Gln13TAG 7D12

Tags

His-Tag

Expression

Bacterial

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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Reb_207 aka PAS630

Plasmid

#71235

Purpose

Reb_206 with RebA(S88P)

Depositor

Pamela Silver

Publication

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

mNeonGreen to second copy RebB, soluble mCherry a…

Expression

Bacterial

Mutation

RebA(S88P)

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP43-2A(sfGFP)

Plasmid

#126987

Purpose

Level 2 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 S12xQ

Plasmid

#127193

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 S12xQ (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

S12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJKW0957

Plasmid

#119026

Purpose

E.coli expression plasmid for PmKOMT1

Depositor

Jing-Ke Weng

Publication

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmKOMT1

Tags

8xHis

Expression

Bacterial

Promoter

T7

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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PC4_3spc34h

Plasmid

#70700

Purpose

Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

Depositor

Stephen Harrison

Publication

Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

Insert

DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)

Tags

his-tag on C-terminus of spc34

Expression

Bacterial

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pET_SZ1-RGG-RGG

Plasmid

#124942

Purpose

Expression of SZ1-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Expression

Bacterial

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET_SZ1-RGG-GFP-RGG

Plasmid

#124937

Purpose

Expression of SZ1-RGG-GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_53

Plasmid

#109251

Purpose

pUC BbsI cloning vector

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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His-Sin3a-115-212(pL01)

Plasmid

#124386

Purpose

E.Coli produced SIN3A PAH1 purified by FPLC nickel column

Depositor

Shaun Cowley

Publication

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

His-Sin3a-115-212 (SIN3A )

Tags

His

Expression

Bacterial

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCAM105

Plasmid

#86479

Purpose

Expresses p9-FLAG-FMO

Depositor

Pamela Silver

Publication

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p9-FLAG-FMO

Use

Synthetic Biology

Tags

FLAG and FMO

Expression

Bacterial

Promoter

pTet

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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As0

Plasmid

#123158

Purpose

Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

Depositor

Baojun Wang

Publication

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR-ABS67, PhrpLE, PrinA

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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