30,880 results

1. pGEX-4T1-Rac1 G15A

   Plasmid

   #69355

   Purpose

   expression of nucleotide free Rac1 in bacterial cells

   Depositor

   Rafael Garcia-Mata

   Article

   Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

   Insert

   Rac1 (RAC1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G15A

   Promoter

   tac

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pHAGE2-TetOminiCMV-OS

   Plasmid

   #136576

   Purpose

   Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIB168

   Plasmid

   #90191

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Tags

   None

   Expression

   Bacterial

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. XNOR-gate-V2.0

   Plasmid

   #44454

   Depositor

   Drew Endy

   Article

   Bonnet et al Science. 2013 May 3;340(6132):599-603. d

   Insert

   XNOR-gate
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTriEx-mCherry-Zdk1-RhoA Q63L

   Plasmid

   #81058

   Purpose

   mammalian expression of Zdk1 tagged RhoA Q63L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   RhoA Q63L (RHOA Human)
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. L1-R184Q

   Plasmid

   #15501

   Depositor

   Vance Lemmon

   Article

   Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

   Insert

   L1-R184Q (L1CAM Human)
   

   Expression

   Bacterial

   Mutation

   Arginine 184 changed to Glutamine

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pET22b-EC12WTC9AAvi

   Plasmid

   #47925

   Purpose

   Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

   Depositor

   Tanja Kortemme

   Article

   Ritterson et al J Am Chem Soc. 2013 Aug 7.

   Insert

   E-Cadherin EC12
   

   Tags

   6xHis, Avi Tag, and TEV Cleavage

   Expression

   Bacterial

   Mutation

   changed Cysteine 9 to Alanine

   Promoter

   T7

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pALS3-Ma-SUMO-sfGFP-WT

   Plasmid

   #212122

   Purpose

   Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   SUMO-sfGFP-WT
   M. alvus Pyl-tRNA(6)
   

   Tags

   His6 and SUMO

   Expression

   Bacterial

   Promoter

   araC and lpp

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pALS3-Ma-SUMO-TAG35-sfGFP

   Plasmid

   #212120

   Purpose

   Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   SUMO-TAG35-sfGFP
   M. alvus Pyl-tRNA
   

   Tags

   His6 and SUMO-TAG35

   Expression

   Bacterial

   Mutation

   E35TAG (within SUMO encoding sequence)

   Promoter

   araC and lpp

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDK637

    Plasmid

    #134203

    Purpose

    bacterial expression of human CENP-N 1-240

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-N 1-240-His (CENPN Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. H6SUMO-IntC-P53(304-393)

    Plasmid

    #200316

    Purpose

    bacterial expression of P53 TET-CTD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (304-393) (TP53 Human)
    

    Tags

    His6-SUMO and designed intein C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. H6GB1tevP53(1-303)IntN

    Plasmid

    #200312

    Purpose

    Bacterial expression of P53 NTAD-DBD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Tags

    His6GB1tev and designed intein N

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSEL1

    Plasmid

    #23011

    Depositor

    James Bowie

    Article

    Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

    Insert

    DHFR (Dhfr Mouse)
    

    Tags

    6 His Tag (not in frame) and Xpress epitope (not …

    Expression

    Bacterial

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pJ4M_hnRNPA2_FL_D290V

    Plasmid

    #139110

    Purpose

    Express FL hnRNPA2 D290V with a C-terminal MBP tag

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Expression

    Bacterial

    Mutation

    D290V

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. ATAD2

    Plasmid

    #38916

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ATAD2 (ATAD2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTWIN1-His6-Ssp-Httex1-7Q

    Plasmid

    #84347

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal His6-tagged intein Ssp

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL1-mKalama1

    Plasmid

    #102639

    Purpose

    In vitro expression of mKalama1 from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

    Insert

    mKalama1
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZ003 (LshC2c2 locus)

    Plasmid

    #70168

    Purpose

    Expresses LshC2c2 full locus

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    LshC2c2 full locus
    

    Expression

    Bacterial

    Available Since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-HiGlpG

    Plasmid

    #158538

    Purpose

    Bacterial expression of rhomboid protease HiGlpG

    Depositor

    Sinisa Urban

    Article

    Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

    Insert

    HiGlpG
    

    Tags

    GST tag

    Expression

    Bacterial

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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