30,880 results

1. pHsSR beta deltaN Flag

   Plasmid

   #13731

   Depositor

   Thomas Schwartz

   Article

   Schwartz et al Proc Natl Acad Sci U S A. 2006 May 2. 10

   Insert

   Signal recognition particle receptor β subunit (SRPRB Human)
   

   Tags

   6xHis and FLAG

   Expression

   Bacterial

   Mutation

   expresses residues 59-271 of Homo sapiens SRβ wit…

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMN234

   Plasmid

   #32108

   Depositor

   Michael Niederweis

   Article

   Stephan et al Gene. 2004 Dec 8;343(1):181-90.

   Insert

   flpe
   

   Expression

   Bacterial

   Promoter

   imyc

   Available Since

   Nov. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR201-Eps8L2

   Plasmid

   #196526

   Purpose

   Entry vector for Gateway with Eps8L2

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Eps8L2-FL (EPS8L2 Human)
   

   Expression

   Bacterial

   Promoter

   CMV promoter; T7 promoter

   Available Since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046733

   Plasmid

   #145665

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. HTRA1_PDZ_1

   Plasmid

   #103912

   Purpose

   Protein expression and purification of HTRA1 PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   HTRA1 (HTRA1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

   Plasmid

   #171795

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET41a iGluSnFR

   Plasmid

   #119828

   Purpose

   Production of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coli

   Depositor

   Katalin Torok

   Article

   Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

   Insert

   iGluSnFR
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJAK2.D

   Plasmid

   #29465

   Depositor

   Zakaria Hmama

   Article

   Sun et al Plasmid. 2009 Nov . 62(3):158-65.

   Type

   Empty backbone

   Use

   Mycobacteria expression

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Acetamide promoter

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. Reb_207 aka PAS630

   Plasmid

   #71235

   Purpose

   Reb_206 with RebA(S88P)

   Depositor

   Pamela Silver

   Article

   Polka et al ACS Synth Biol. 2016 Feb 3.

   Insert

   Reb locus
   

   Tags

   mNeonGreen to second copy RebB, soluble mCherry a…

   Expression

   Bacterial

   Mutation

   RebA(S88P)

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOSV802

    Plasmid

    #126595

    Purpose

    modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

    Depositor

    Jean-Luc Pernodet

    Article

    Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHYDuet183

    Plasmid

    #41685

    Depositor

    Hideo Iwai

    Article

    Ellila et al FEBS Lett. 2011 Oct 10.

    Insert

    PhoRadA intein
    

    Tags

    GB1 and His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBRBB-Ppuf843-1200-PR

    Plasmid

    #50963

    Purpose

    Expresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroides

    Depositor

    Claudia Schmidt-dannert

    Article

    Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

    Insert

    Proteorhodopsin
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Ppuf843-1200

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid

    #128404

    Purpose

    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A (toxA P. aeruginosa)
    

    Tags

    6xHis tag and DsbA signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3521 pET ubcH6

    Plasmid

    #8653

    Depositor

    Peter Howley

    Article

    Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

    Insert

    UbcH6 (UBE2E1 Human)
    

    Expression

    Bacterial

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

15. PdnaK-IR3-IR3-Bxb1-CadR-pZa

    Plasmid

    #204686

    Purpose

    Cadmium responsive genetic circuit

    Depositor

    Urartu Seker

    Article

    Akboga et al Biosensors (Basel). 2022 Feb 16;12(2):12

    Insert

    Bxb1 (Bxb1p45 Synthetic)
    

    Use

    Synthetic Biology

    Tags

    CadR

    Expression

    Bacterial

    Promoter

    Inducible, HspR is its repressor

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVRc20_992

    Plasmid

    #49739

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    AS20_992
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pLux

    Available Since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. GST-FOXO1

    Plasmid

    #24379

    Depositor

    Anne Brunet

    Insert

    Foxo1 (Foxo1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOGG010

    Plasmid

    #113984

    Purpose

    Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    RK2 (IncP)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM33_CLTC_Clathrin propeller

    Plasmid

    #178471

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CLTC_Clathrin propeller (CLTC Synthetic, Human)
    

    Tags

    His-GST

    Expression

    Bacterial

    Promoter

    T7/LacO

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET19-MTdTc302

    Plasmid

    #207458

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc302 (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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