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pET-Duet-1-NONO(53-312)

Plasmid

#135442

Purpose

Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

Non-POU domain-containing octamer-binding protein (NONO Human)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Sc RFC5-pCDFDuet

Plasmid

#239202

Purpose

Overexpress Sc RFC5 in E. coli

Depositor

Michael O'Donnell

Insert

RFC5 (RFC5 Budding Yeast)

Expression

Bacterial

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_9C74

Plasmid

#234238

Purpose

Heterologous protein expression of variant of super folded GFP in Escherichia coli

Depositor

Christopher Bahl

Insert

9C74

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEM8731

Plasmid

#213991

Purpose

PrpsM driven expression of mNeonGreen - sequence codon-optimized for Salmonella

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

Plasmid

#199781

Purpose

Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

Depositor

Sascha Martens

Insert

NEMO (IKBKG Human)

Tags

GST-Thrombin-TEV-EGFP

Expression

Bacterial

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBSV2_PresT-mCherryBb

Plasmid

#118238

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

resT promoter driven mCherry

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b_Q69L

Plasmid

#186272

Purpose

Expression vector of His-RanQ69L in E.coli

Depositor

Masahito Ikawa

Article

Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

GTP-binding nuclear protein Ran (Ran Mouse)

Tags

His-tagged at N terminal

Expression

Bacterial

Mutation

Changed Q69 into L

Promoter

T7 promoter

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-HS

Plasmid

#133305

Purpose

"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

hok/sok toxin-antitoxin cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCPP6534

Plasmid

#128735

Purpose

Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag

Depositor

Alan Collmer

Article

Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK1008 (pSL7)

Plasmid

#191003

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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