30,256 results

1. pBW400hrpL-gfp

   Plasmid

   #61437

   Purpose

   pBW400hrpL-gfp

   Depositor

   Martin Buck, Baojun Wang

   Article

   Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

   Insert

   hrpL-rbs30-gfp-ter
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   HrpL

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET26b(+)-ATI_QconCAT_1

   Plasmid

   #163957

   Purpose

   Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

   Depositor

   Stefan Tenzer

   Article

   Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

   Insert

   ATI QconCAT
   

   Use

   Tags

   polyhistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. HAO1

   Plasmid

   #38970

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HAO1 (HAO1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-T7-Bottom-T

   Plasmid

   #87310

   Purpose

   Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system "Bottom" transcript
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. human p53-(310-360)

   Plasmid

   #24872

   Depositor

   Cheryl Arrowsmith

   Article

   Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains only the dimeric oligomerization domain …

   Promoter

   Available since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pOPINK-TRABID (NZF1, aa 1-33)

   Plasmid

   #65437

   Purpose

   Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   TRABID (ZRANB1 Human)
   

   Use

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   only codes for amino acids 1-33 of TRABID, which …

   Promoter

   T7

   Available since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pWH-DI

   Plasmid

   #47385

   Purpose

   Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Inserts

   dxs
   idi
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PxylA

   Available since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pBAD-pHoran4

   Plasmid

   #61558

   Purpose

   Orange fluorescent protein based pH indicator in bacterial expression vector

   Depositor

   Robert Campbell

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   pHoran4
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

9. GLS2A-c007

   Plasmid

   #53621

   Purpose

   bacterial expression plasmid

   Depositor

   Nicola Burgess-Brown

   Insert

   GLS2A
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pOPINO_C1

    Plasmid

    #171924

    Purpose

    Bacterial expression of nanobody VHH_C1, which binds to receptor binding domain of the spike protein of SARS-CoV-2

    Depositor

    Ray Owens

    Article

    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

    Insert

    VHH_C1
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. BBa_J72114-BBa_J72100

    Plasmid

    #40781

    Depositor

    Christopher Anderson

    Article

    Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

    Insert

    lacZ
    

    Use

    Synthetic Biology; Phagemid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CaP2AIII_pK18

    Plasmid

    #153421

    Purpose

    Source of murine TCR-alpha constant region (TRAC*02) gene followed by P2A

    Depositor

    Maki Nakayama

    Article

    Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    murine TCR-alpha constant region (TRAC*02)-P2A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a-mAIM2-His6

    Plasmid

    #51536

    Purpose

    Express His6-tagged mAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    Aim2 (Aim2 Mouse)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. BLVRA

    Plasmid

    #38856

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BLVRA (Blvra Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pC239

    Plasmid

    #65953

    Purpose

    N2 inverter plasmid for two-strain system

    Depositor

    Matthew Bennett

    Article

    Chen et al Science. 2015 Aug 28;349(6251):986-9. do

    Inserts

    AiiA
    LacI
    

    Use

    Tags

    LAA tagged

    Expression

    Bacterial

    Mutation

    Promoter

    pCin and pCin*

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Erk1

    Plasmid

    #29578

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    Erk1 (MAPK3 Human)
    

    Use

    Tags

    FLAG, HIS6, and TEV

    Expression

    Bacterial

    Mutation

    K289N mutation present. Starting Met removed from…

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

17. p264 pSP72 hAR-LBD(LNCaP)

    Plasmid

    #12495

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Insert

    AR LBD (AR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr 878 Ala in AR-LBD

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pET31b-T7-Spinach2-Ct

    Plasmid

    #79158

    Purpose

    Expresses the Ct biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Ct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-S9

    Plasmid

    #132948

    Purpose

    Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S9 (rpsI E.coli)
    

    Use

    Tags

    N-terminal 6xHis-tag

    Expression

    Bacterial

    Mutation

    E52C

    Promoter

    T7

    Available since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PclpB GFP pZa

    Plasmid

    #170074

    Purpose

    GFP expression under the control of E. coli clpB promoter

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PclpB

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge