30,880 results

1. NEDD4L

   Plasmid

   #25169

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   nedd4l.0574.0947 (NEDD4L Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Hect Domain

   Available Since

   Aug. 9, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pGBK CUP1 EGFP

   Plasmid

   #170210

   Purpose

   Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFP

   Depositor

   Bruce Hamilton

   Article

   Hernandez et al bioRxiv

   Insert

   enhanced green fluorescent protein
   

   Tags

   GAL4 DNA Binding Domain and Myc

   Expression

   Bacterial and Yeast

   Promoter

   CUP1

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-hsGFP

   Plasmid

   #64911

   Purpose

   Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli

   Depositor

   Huiwang Ai

   Article

   Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

   Insert

   hsGFP
   

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Chromophore tyrosine of the protein mutated to TA…

   Promoter

   araBAD

   Available Since

   May 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-AC3CtermGenomic-mCherry

   Plasmid

   #105250

   Purpose

   mouse mCherry (with STOP and extra-sequence) with homology arms for Adcy3

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Proc Natl Acad Sci U S A. 2017 Nov 28. p

   Insert

   mCherry (with STOP and extra-sequence) with homology arms for mouse Adcy3 (Adcy3 Mouse)
   

   Expression

   Bacterial

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYTK077

   Plasmid

   #65184

   Purpose

   Encodes KanamycinR as a Type 6 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   KanamycinR
   

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. araC-pBAD-gusA-pBAV1k

   Plasmid

   #30502

   Depositor

   Ichiro Matsumura

   Article

   Murin et al Appl Environ Microbiol. 2011 Oct 21.

   Insert

   beta-glucuronidase (gusA Escherichia coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Oct. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTetQCas-BsaI

   Plasmid

   #170629

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
   

   Expression

   Bacterial

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR2B-dCas9-KRAB

   Plasmid

   #115547

   Purpose

   Entry cassette for dCas9-KRAB

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Insert

   dCas9-KRAB
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103 BAG3 WW domain

   Plasmid

   #104266

   Purpose

   Bacterial expression of WW domain from BAG3

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   BAG3 WW domain (BAG3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRCHisB-TRF2∆M

    Plasmid

    #50489

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Delta Myb (TERF2 Human)
    

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 3-437

    Promoter

    pTrc

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pReporter_5

    Plasmid

    #60566

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_5
    

    Expression

    Bacterial

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30a-SARS-CoV2-NP-Nt(47-173)

    Plasmid

    #165091

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMAL-c2x-MT1

    Plasmid

    #32101

    Depositor

    David DeRosier, Chris Mercogliano, Cristina Risco Ortiz

    Article

    Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

    Insert

    Metallothionein (Mt1 Mouse)
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    P-lac

    Available Since

    Jan. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pET28a(cd226)

    Plasmid

    #177900

    Purpose

    Bacterial expression of his10-tagged human cd226 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    CD226 (aa 288-336 only) (CD226 Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSK-c-mpl

    Plasmid

    #1828

    Depositor

    Philip Leder

    Article

    Skoda et al EMBO J 1993 Jul;12(7):2645-53.

    Insert

    c-mpl (Mpl Mouse)
    

    Expression

    Bacterial

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJKR-L-mphR

    Plasmid

    #62560

    Purpose

    Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4046916

    Plasmid

    #145627

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pEB1-mEYFP

    Plasmid

    #103985

    Purpose

    Plasmid encoding mEYFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mEYFP
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S65G, V68L, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HS-OS-Plk4-mKate2

    Plasmid

    #105944

    Purpose

    heat shock inducible transgenesis, non phoshorylable version of Plk4

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Plk4 (Plk4 Mouse)
    

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    1002 to 3704 of Plk4/ deletion of S282-S305.

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-PLS-KP6_rpoA-U

    Plasmid

    #190967

    Purpose

    Test the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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