30,880 results

1. TLS 1: hTLS.pBSKS(+)

   Plasmid

   #21827

   Depositor

   David Ron

   Article

   Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

   Insert

   TLS (FUS Human)
   

   Expression

   Bacterial

   Available Since

   Sept. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. PTPN13_PDZ_4

   Plasmid

   #103922

   Purpose

   Protein expression and purification of PTN13 PDZ4 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PTPN13 (PTPN13 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL0426 (CRISPR with spc3)

   Plasmid

   #130642

   Purpose

   Expresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA with spacer 3 from the native CRISPR.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   CRISPR(spacer-3)
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6p-1-PafA-XIAP

   Plasmid

   #113402

   Purpose

   Expresses PafA-XIAP in bacteria

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   PafA-XIAP
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

   Plasmid

   #101230

   Purpose

   Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

   Depositor

   Jennifer Doudna, Keith Joung

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
   

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

   Promoter

   T7

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. SIRT6.27

   Plasmid

   #13739

   Depositor

   John Denu

   Article

   Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

   Insert

   SIRT6 (SIRT6 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pOPINO_C5

   Plasmid

   #171925

   Purpose

   Bacterial expression of nanobody VHH_C5, which binds to receptor binding domain of the spike protein of SARS-CoV-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

   Insert

   VHH_C5
   

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Sept. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pACYC.Taq.1Amb

   Plasmid

   #102794

   Purpose

   Expresses Taq DNA polymerases with a premature stop codon

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   Taq DNA polymerase
   

   Expression

   Bacterial

   Mutation

   W168Stop; *(see below)

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMCSG53_Nsp15

   Plasmid

   #167265

   Purpose

   Bacterial expression of Nsp15

   Depositor

   Alexei Savchenko

   Insert

   Nsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
   

   Tags

   His6-TEV site

   Expression

   Bacterial

   Mutation

   none

   Available Since

   May 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET28a-Beclin1 CC

    Plasmid

    #24403

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains the CC domain (aa 151-242)

    Available Since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

11. hOtub1

    Plasmid

    #26959

    Depositor

    Cynthia Wolberger

    Article

    Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

    Insert

    Otub1 (OTUB1 Human)
    

    Tags

    His6 (TEV cleavable)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

12. Ubiquitin I44A

    Plasmid

    #12648

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    Ubiquitin (UBC Human)
    

    Expression

    Bacterial

    Mutation

    Ile44Ala

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pBZcsx27

    Plasmid

    #89900

    Purpose

    Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    csx27
    

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

    Plasmid

    #130639

    Purpose

    Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on Cas8

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETDuet-1 TIM8,13

    Plasmid

    #170282

    Purpose

    Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

    Depositor

    Paul Schanda

    Article

    Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

    Insert

    yeast Tim8 and Tim13 (TIM8 Budding Yeast)
    

    Tags

    TEV-cleavable N-terminal His tag on Tim13 (on bac…

    Expression

    Bacterial

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET11a-STEPtag

    Plasmid

    #159373

    Purpose

    Expresses STEPtag in E. coli

    Depositor

    Roberto Chica

    Article

    Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

    Insert

    STEPtag
    

    Tags

    6x His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b-BE3∆UGI

    Plasmid

    #96943

    Purpose

    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

    Depositor

    Daesik Kim

    Article

    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

    Insert

    BE3∆UGI
    

    Tags

    His6 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. bactin:GFP-PCNA

    Plasmid

    #105979

    Purpose

    transgenesis, ubiquitous expression

    Depositor

    Caren Norden

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    PCNA (PCNA Human)
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEVA231-CRISPR

    Plasmid

    #138712

    Purpose

    Plasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR Array

    Depositor

    Víctor de Lorenzo

    Article

    Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. KTK_354

    Plasmid

    #180525

    Purpose

    KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.

    Depositor

    Tom Ellis

    Article

    Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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