30,810 results

1. pGEX SHP-2(N)-SH2

   Plasmid

   #46498

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(N) (PTPN11 Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-104

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExp-PpiB

   Plasmid

   #112572

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Tags

   8xHis-PpiB and Strep tag

   Expression

   Bacterial

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBD-0016

   Plasmid

   #12345

   Depositor

   Axel Brunger

   Article

   DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

   Insert

   VCP R313K (Vcp Mouse)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R313K, I206V*(see below)

   Available Since

   Aug. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pHybr_r2a>mG2a-srt-his

   Plasmid

   #124803

   Purpose

   HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgG2a
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Erk1

   Plasmid

   #29578

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

   Insert

   Erk1 (MAPK3 Human)
   

   Tags

   FLAG, HIS6, and TEV

   Expression

   Bacterial

   Mutation

   K289N mutation present. Starting Met removed from…

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pAM002

   Plasmid

   #170006

   Purpose

   Encodes PpmrD-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   Superfolder GFP
   PhoP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLTetO-1 and PpmrD

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaG-29

   Plasmid

   #172753

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-29; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-29
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-His-hPKM2

   Plasmid

   #42515

   Depositor

   Dimitrios Anastasiou

   Article

   Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

   Insert

   Pyruvate Kinase M2 (PKM Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pG1AK-mCherry

   Plasmid

   #71737

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Insert

   mCherry
   

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Bacterial

   Promoter

   pRplsWT

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. B1-2

    Plasmid

    #111464

    Purpose

    Met-tRNA synthetase

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    Met-tRNA synthetase
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

    Plasmid

    #192273

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBLO 4.01

    Plasmid

    #121495

    Purpose

    engineered Mu transposon

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    p15A

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCOLA-T7-WspR:G249A

    Plasmid

    #79163

    Purpose

    Expresses inactive WspR G249A under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    WspR (wspR P. areuginosa)
    

    Expression

    Bacterial

    Mutation

    G249A

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1_Cre

    Plasmid

    #201198

    Purpose

    AAV vector for cre expression under EF1 promoter

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Inserts

    cre
    EF1alpha promoter
    

    Use

    AAV

    Expression

    Bacterial and Mammalian

    Mutation

    nuclear targeting cre

    Promoter

    N/A and hEF1alpha promoter

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMOD3-mClover-nat

    Plasmid

    #120328

    Purpose

    Bacterial mClover promoter trap

    Depositor

    James Kirby

    Article

    Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

    Insert

    mClover
    

    Expression

    Bacterial

    Mutation

    D103N (see comments below)

    Promoter

    (None)

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVRb01_syn20

    Plasmid

    #49704

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    sfgfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Pecf01_syn20

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-tTALE-mStrawberry-15b

    Plasmid

    #206868

    Purpose

    Express tTALE-mStrawberry

    Depositor

    Kyubong Jo

    Article

    Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

    Insert

    7-mer tTALE-mStrawberry
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC012 LwCas13a locus with nontargeting spacer

    Plasmid

    #91904

    Purpose

    LwCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LwCas13a with non-targeting spacer
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Human HMG-CoA lyase (HL)

    Plasmid

    #52433

    Purpose

    bacterial expression of human HMG-CoA lyase (HL)

    Depositor

    Henry Miziorko

    Article

    Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

    Insert

    HMG CoA lyase (HMGCL Human)
    

    Tags

    His and S

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pTXTL-T7max-LuxA

    Plasmid

    #188305

    Purpose

    Expression of LuxA via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    luxA
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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