30,808 results

1. pACYC.GFP1Amb

   Plasmid

   #102792

   Purpose

   Expresses GFP with a premature stop codon

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   Green Fluorescent Protein
   

   Expression

   Bacterial

   Mutation

   Y39Stop

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. ZP17 Krox20

   Plasmid

   #16924

   Depositor

   Randall Moon

   Insert

   Krox20 (egr2b Zebrafish)
   

   Expression

   Bacterial

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pRO16Sh2

   Plasmid

   #180604

   Purpose

   Expression of ShCAST under control of Plac with pRO1600 origin.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLac

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSG81-pLas-tetO-deGFPssrA

   Plasmid

   #102454

   Purpose

   Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pLas-tetO combinatorial promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   deGFP
   

   Tags

   ssrA

   Expression

   Bacterial

   Promoter

   pLas-tetO

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLBH547_Tet-Cas14b2Locus

   Plasmid

   #112504

   Purpose

   Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b2
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T_PDZ2

   Plasmid

   #59735

   Purpose

   expresses PDZ2 domain of human ZO-1 in bacteria

   Depositor

   Alan Fanning

   Article

   Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6

   Insert

   zonula occludens-1 (TJP1 Human)
   

   Tags

   GST and thrombin clvg

   Expression

   Bacterial

   Available Since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pXWP11-gfp2

   Plasmid

   #123152

   Purpose

   pSB1K3 carrying Pecf11-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   Pecf11-30gfp-t
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Pecf11

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T Syt1 C2AB(140) WT

   Plasmid

   #134695

   Purpose

   Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression and purification

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Synaptotagmin 1 (Syt1 Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hp MTAN

   Plasmid

   #69802

   Purpose

   Expression of H. pylori J99 methylthioadenosine nucleosidase

   Depositor

   Vern Schramm

   Article

   Wang et al Biochemistry. 2012 Sep 4;51(35):6892-4.

   Insert

   Methylthioadenosine nucleosidase
   

   Tags

   Histidine tag

   Expression

   Bacterial

   Available Since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21TtTERT(195-516)

    Plasmid

    #28172

    Depositor

    Kathleen Collins

    Article

    O'Connor et al J Biol Chem. 2005 Apr 29. 280(17):17533-

    Insert

    Tetrahymena TERT
    

    Expression

    Bacterial

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pYTK033

    Plasmid

    #65140

    Purpose

    Encodes Venus as a Type 3 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    Venus
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX Abl-SH2

    Plasmid

    #46402

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Abl (ABL1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 120-225

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. NM-01

    Plasmid

    #69799

    Purpose

    Bacterial expression of Cas9 with His, MBP, and Strep tags

    Depositor

    Gerard Cagney

    Insert

    Cas9
    

    Tags

    HIS, MBP, and StrepII

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pBbB7a-GFP

    Plasmid

    #35358

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9-M-G6

    Plasmid

    #73437

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor G6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(TIGIT)

    Plasmid

    #177902

    Purpose

    Bacterial expression of his10-tagged human TIGIT Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    TIGIT (aa 163-244 only) (TIGIT Human)
    

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHH0103_ALDOA_Glycolytic

    Plasmid

    #109854

    Purpose

    Protein expression and purification of ALDOA_Glycolytic

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ALDOA_Glycolytic (ALDOA Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSAVED-CHAT_PCaspase_PCi_PC-σ

    Plasmid

    #214040

    Purpose

    Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-Pcaspase-Pci-PC-σ
    

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21(+)-His6-Ubc13

    Plasmid

    #212715

    Purpose

    Bacterial expression of His6-Ubc13

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    Ubc13
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAllet

    Plasmid

    #202560

    Purpose

    E. coli cloning vector with large MCS (50+ unique restriction sites)

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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