32,686 results
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Plasmid#113318PurposeAraC-pBAD-dCas9, MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRExpressionBacterialMutationR93AAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
4LG1
Plasmid#60092PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL21D:JMC072-C04:C227980 (VCPKMT Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNRB150
Plasmid#36451DepositorAvailable SinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #3
Plasmid#104210PurposeBacterial expression of WW domain #3 from NEDD4DepositorInsertNEDD4 WW domain #3 (NEDD4 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mEYFP
Plasmid#103985PurposePlasmid encoding mEYFPDepositorInsertmEYFP
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S65G, V68L, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tensin-2 WT
Plasmid#201797PurposeExpresses wild-type Tensin-2 protein in MAC-Tag-C backboneDepositorAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5400_pMBP_CDS dropout vector
Plasmid#221189PurposeExpression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interestDepositorTypeEmpty backboneTags10x His-tag + E. coli maltose binding protein (MB…ExpressionBacterialPromoterT7 promoterAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJCC_083 Cas12i1
Plasmid#171670PurposeFor bacterial expression of Cas12i1 with an N-terminal His-MBP tagDepositorInsertCas12i1
Tags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA-DAB2
Plasmid#162690PurposeExpresses DabAB2 inorganic carbon transport complexDepositorInsertDabAB2 inorganic carbon transport complex
ExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXPR-SF61
Plasmid#163916PurposetRNA synthetase/tRNA pair for the in vivo incorporation of para-Pentafluorosulfanyl-Phenylalanine, into proteins in E. coli in response to the amber (TAG) codonDepositorInsertSF5Phe tRNA synthetase
ExpressionBacterialPromoteraraBADAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero11
Plasmid#187928PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero11DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
p_SNIPR_target_WT
Plasmid#139465PurposeT7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensorDepositorInsertTarget_WT
ExpressionBacterialAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
Reb_207 aka PAS630
Plasmid#71235PurposeReb_206 with RebA(S88P)DepositorInsertReb locus
TagsmNeonGreen to second copy RebB, soluble mCherry a…ExpressionBacterialMutationRebA(S88P)Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-PTB
Plasmid#21929DepositorAvailable SinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMD18-T-pyrF
Plasmid#174388Purposemodified pMD18-T containing pyrF and its native promoter, AmprDepositorInsertpyrF
UseTemplate for cloning fragments for chromosomal in…ExpressionBacterialAvailable SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-citrate
Plasmid#225251PurposeBacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrateDepositorInsertqmTQ2-citrate
Tags6XHis, T7 tag, XpressTM tagExpressionBacterialPromoterT7 promoterAvailable SinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207in
Plasmid#176230PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTmpKmH207out
Plasmid#176231PurposeA template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.DepositorInsertcI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only