30,808 results

1. p264 pSP72 hAR-LBD(LNCaP)

   Plasmid

   #12495

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

   Insert

   AR LBD (AR Human)
   

   Expression

   Bacterial

   Mutation

   Thr 878 Ala in AR-LBD

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pET21b-RL015A

   Plasmid

   #42134

   Depositor

   Sheref Mansy

   Article

   Lentini et al ACS Synth Biol. 2013 Mar 15.

   Insert

   sfGFP-mCherry
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Feb. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-PI(WT)-Src(KD)-mCherry

   Plasmid

   #87358

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Src (Src Mouse)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   D388R

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBetI-E1

   Plasmid

   #74677

   Purpose

   BetI-E1 gate

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

   Insert

   PTac-RBSE1-BetI-PBetI-YFP
   

   Expression

   Bacterial

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSW002-PpsbA-E2-Crimson

   Plasmid

   #111258

   Purpose

   Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with E2-Crimson

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   E2-Crimson
   

   Expression

   Bacterial

   Promoter

   PpsbA

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. STK3_HUMAN_D0

   Plasmid

   #79689

   Purpose

   This plasmid encodes the kinase domain of STK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   STK3 (STK3 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

   Plasmid

   #116963

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3I91

   Plasmid

   #62514

   Purpose

   Bacterial expression plasmid for use in structural determination.

   Depositor

   Cheryl Arrowsmith

   Article

   Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

   Insert

   CBX8 (CBX8 Human)
   

   Tags

   6xHis and TEV cleavage

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pET24a MDHC1_ARATH

   Plasmid

   #204237

   Purpose

   Bacterial expression of Arabidopsis cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHC1_ARATH (AT1G04410 Mustard Weed)
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500

    Plasmid

    #92228

    Purpose

    Expression of zmADH using the p70a promoter and UTR1

    Depositor

    Lital Alfonta

    Article

    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

    Insert

    NTerm 6xHis Tagged zmADH
    

    Use

    Synthetic Biology; Cell free protein synthesis (c…

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    p70a

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. GST-N1IC

    Plasmid

    #47612

    Purpose

    bacterial expression of GST-myc-Notch1 IC

    Depositor

    Urban Lendahl

    Article

    Beatus et al Development. 1999 Sep;126(17):3925-35.

    Insert

    Notch1 intracellular domain (Notch1 Mouse)
    

    Tags

    GST and myc

    Expression

    Bacterial

    Mutation

    contains amino acids 1753-2531

    Promoter

    tac

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pDB88

    Plasmid

    #26958

    Depositor

    Michael Glickman

    Article

    Barkan et al Gene. 2011 Jan 1;470(1-2):31-6. doi: 10.

    Insert

    e. coli galactokinase
    

    Expression

    Bacterial

    Mutation

    cloned under the MOP promoter

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. env8 selectivity (b')

    Plasmid

    #99837

    Purpose

    mNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.

    Depositor

    Robert Batey

    Article

    Polaski et al J Biol Chem. 2017 May 8. pii: jbc.M117.7

    Insert

    env8 selectivity (b')
    

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    A20G, A68G, G-C pairs in P1 and P3

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pgRNAtet-phaZ

    Plasmid

    #169874

    Purpose

    Single guide RNA plasmid for Cas9 targeting phaZ in P. putida

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    sgRNA towards phaZ in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS18: pHelper(PmcCAST)_PmcPSP1

    Plasmid

    #168151

    Purpose

    Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    PmcTniQ
    

    Expression

    Bacterial

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEB2-TurboRFP

    Plasmid

    #103996

    Purpose

    Plasmid encoding TurboRFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TurboRFP
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, T68A, L79F, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXN-FBLWLF-LexA

    Plasmid

    #26266

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    LexA
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4092709

    Plasmid

    #153859

    Purpose

    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    Nearly native encoding;handful SNPs as watermarks…

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pGEX-4T-1-β4 hinge-ear

    Plasmid

    #196692

    Purpose

    Expresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteria

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2015 Dec 25;290(52):30736-4

    Insert

    AP-4 β4 hinge-ear (534-739) (AP4B1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD-LSSA12

    Plasmid

    #186546

    Purpose

    LSSA12 fluorescent protein (ex/em = 398/511 nm): bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSA12
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hfYFP-G65S/Y203I/Q204E/E222D/R223F

    Promoter

    araB

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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