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pWUR703

Plasmid

#53082

Purpose

inducible TtAgoDM (D478A,D546A) expression

Depositor

John van der Oost

Article

Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

Insert

TtAgo

Tags

Strep-II tag

Expression

Bacterial

Mutation

D478A D546A

Promoter

T7

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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JMJD2A

Plasmid

#38846

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

JMJD2A (Kdm4a Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp15-StefinA

Plasmid

#182405

Purpose

Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-stefin (CSTA Human)

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBbA2c-RFP

Plasmid

#35326

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCM18

Plasmid

#78565

Purpose

60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmid

Depositor

James Kaper

Article

Morin et al FEMS Immunol Med Microbiol. 2009 Oct;57(

Type

Empty backbone

Tags

luxCDABE

Expression

Bacterial

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-CFP

Plasmid

#90103

Purpose

CFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

CFP

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pRDV5 containing Sb-convex

Plasmid

#132696

Purpose

pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_convex including 5' and 3' flanking sequences used for ribosome display

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET-45b-POLR2A-CTD-mCherry

Plasmid

#185011

Purpose

Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

POLR2A (Polr2a Mouse)

Expression

Bacterial

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHLUM (version 2)

Plasmid

#64166

Purpose

harbours marker genes HIS3, LEU2, URA3 and MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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