32,967 results
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Plasmid#128724PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopY1
ExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-H253A-R256A-R260A-R296A-R418A-Y419A
Plasmid#127790PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in His2…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5530
Plasmid#128705PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopA1
ExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB15a-GFP
Plasmid#126130PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23100Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-MBP-CfaN-His6
Plasmid#126017PurposeBacterial expression of MBP-CfaN fusion protein (with C-terminal His5)DepositorInsertMBP-CfaN-His5
Tags5xHisExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExp-Calm
Plasmid#129238PurposeProtein expression in E.coliDepositorTypeEmpty backboneTagsHis and StrepExpressionBacterialPromoterT7Available SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAA6
Plasmid#128282Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq- mRFP, parS
ExpressionBacterialAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM015
Plasmid#128289Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPara - lp-cfp-parB
ExpressionBacterialAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOSV803
Plasmid#126596Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP27ts-1A(sfGFP)
Plasmid#126975PurposeLevel 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationRepA A56V thermosensitive mutationAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP18-lambdaRED_NOC
Plasmid#127032PurposeBasic Part NOC- Complete ORF; LambdaRED / _RED; Policistronic "NOC" part with coding regions for the three lambda red proteins (the first; gam; doesn't have RBS; but bet and exo genes have RBS).DepositorInsertPart ?RED_NOC
UseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pES133
Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
ExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…Available SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT100.110
Plasmid#122602Purposeclone CDA1 phage by SapI Golden GateDepositorInsertCDA1
ExpressionBacterialAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSPB845
Plasmid#128296Purposeasymmetric plasmid partitioning E. coli - regulatory plasmidDepositorInsertPLlacO1 - lp-cfp-parB, BBa_J23110 - lacI
ExpressionBacterialAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAH01SpR
Plasmid#128159PurposepCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDSDepositorTypeEmpty backboneExpressionBacterialPromoterPtetAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Abeta(MC1-40)
Plasmid#127152PurposeExpresses N-terminal cysteine Abeta(1-40)DepositorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
Tags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP49-2E(sfGFP)
Plasmid#126995PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP48-2A(sfGFP)
Plasmid#126993PurposeLevel 2 vector. sfGFP reporter. Origin pUC (high copy number)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP41-2A(sfGFP)
Plasmid#126985PurposeLevel 2 vector. sfGFP reporter. Origin R6K (pir conditional).DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only