30,736 results

1. N8his-GFPenhancer-GGGGS4-LaG16

   Plasmid

   #140442

   Purpose

   We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.

   Depositor

   Motoyuki Hattori

   Article

   Zhang et al Sci Rep. 2020 Apr 10;10(1):6239. doi: 10

   Insert

   GFPenhancer-GGGGS4-LaG16
   

   Tags

   8 histidine tag

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pON.mCherry

   Plasmid

   #84821

   Purpose

   Constitutive Expression plasmid with mCherry fluorescent protein

   Depositor

   Howard Shuman

   Article

   Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

   Insert

   mCherry
   

   Expression

   Bacterial

   Promoter

   Tac Promoter

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSGKP-spe

   Plasmid

   #117234

   Purpose

   A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET22b-mEB1-mCherry-6xHis

   Plasmid

   #130984

   Purpose

   Bacterial expression of mouse EB1-mCherry-6xHis for protein purification.

   Depositor

   Anne Straube

   Article

   Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

   Insert

   EB1-mCherry-6xHis (Mapre1 Mouse)
   

   Tags

   mCherry-6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-AR-1-660

   Plasmid

   #104196

   Purpose

   Expresses human AR-1-660 from bacterial expression vector pGEX-5X-1 as GST fusion protein

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

   Insert

   human androgen receptor aa 1-660 (AR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAR18_StrepI_mFASm_H8_pET22b

   Plasmid

   #122847

   Purpose

   Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
   

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTD-NTwinStrep_Km

   Plasmid

   #45938

   Purpose

   use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

   Depositor

   Thorben Dammeyer

   Article

   Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

   Type

   Empty backbone

   Tags

   6xHis and Twin-Strep

   Expression

   Bacterial

   Promoter

   Trc (lactose/IPTG inducible)

   Available Since

   Sept. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC18T-mini-Tn7T-Tp-dsRedExpress

   Plasmid

   #65036

   Purpose

   TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   dsRedExpress
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBAD-Lime

    Plasmid

    #191255

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein Lime

    Depositor

    Robert Campbell

    Article

    Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

    Insert

    Lime
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTW335c3

    Plasmid

    #237322

    Purpose

    expresses A. thaliana RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Article

    Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

    Inserts

    A. thaliana RbcL (rbcL Mustard Weed)
    A. thaliana RbcS-6xHis
    

    Tags

    6xHis and None

    Expression

    Bacterial

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-SNAP-PI Probe

    Plasmid

    #211507

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BcPI-PLC-ANH
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TXNRD1

    Plasmid

    #38863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TXNRD1 (Txnrd1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX6P1-mCherry-Nanobody (LaM-2)

    Plasmid

    #162276

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    mCherry-Nanobody (LaM-2)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBBR1k-J23107-GFPuv

    Plasmid

    #106389

    Purpose

    pBBR1k-GFPuv with Anderson promoter J23107 expressing lacI

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T1-RhoA G17A

    Plasmid

    #69357

    Purpose

    expression of nucleotide free RhoA in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    RhoA (RHOA Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G17A

    Promoter

    tac

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pNP566

    Plasmid

    #222724

    Purpose

    pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST39-pNL29

    Plasmid

    #91696

    Purpose

    Expresses Mega GVs in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

    Insert

    Mega GV gene cluster (from pNL29) in pST39 backbone
    

    Tags

    No tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6xHis-SNAP-PI(3,4,5)P3 Probe

    Plasmid

    #211514

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    BTK1-PH
    

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHBintT

    Plasmid

    #48134

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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