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pGEX-Bcatfl

Plasmid

#24193

Depositor

David Rimm

Insert

beta-catenin (CTNNB1 Human)

Tags

GST

Expression

Bacterial

Available since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pcDNA3-HA-Tuba

Plasmid

#22214

Depositor

Pietro De Camilli

Publication

Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tuba (DNMBP Human)

Tags

HA

Expression

Bacterial and Mammalian

Available since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pGEX-2T-Rac1-Q61L

Plasmid

#12979

Depositor

Gary Bokoch

Insert

Rac1 constitutively active (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

Q61L Constitutively active

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pBD-0001

Plasmid

#12373

Depositor

Axel Brunger

Publication

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP (Vcp Mouse)

Tags

His6

Expression

Bacterial

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDW363

Plasmid

#8842

Depositor

David Waugh

Publication

Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

Type

Empty backbone

Tags

MBP and biotin acceptor peptide

Expression

Bacterial

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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1257 pGEX4T3 GST FKHR

Plasmid

#10706

Depositor

William Sellers

Insert

FKHR (FOXO1 Human)

Tags

GST

Expression

Bacterial

Available since

March 14, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
6xHis-SNAP-PI(3)P Probe

Plasmid

#211508

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

FYVE-Hrs x 2 (PIKFYVE Human)

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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6xHis-SNAP-PI(3,5)P2 Probe

Plasmid

#211512

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SnxA-2xPX

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cre

Plasmid

#198274

Purpose

For loading Cre into PVCs; also contains PVC regulatory genes

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Publication

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Expression

Bacterial

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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plRL2

Plasmid

#163631

Purpose

Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Publication

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBbAW4k-loxP-TT-loxP-mRFP1

Plasmid

#134405

Purpose

Fluorescent reporter for Cre recombinase activity. Cre removes a transcription terminator, leading to RFP production.

Depositor

Mary Dunlop

Publication

Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

Insert

W4-loxP-TT-loxP-mRFP1

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

W4 (modified T7A1)

Available since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLp_3050sNuc

Plasmid

#122030

Purpose

Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

Depositor

Vincent Eijsink, Geir Mathiesen

Publication

Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

Insert

signal peptide Lp_3050

Expression

Bacterial

Promoter

sppA

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pQE30-His-ThrRS

Plasmid

#124119

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Threonyl-tRNA ligase (thrS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGPA1

Plasmid

#115476

Purpose

Generation of mariner-based transposon mutant libraries in Enterococcus faecium

Depositor

Willem van Schaik

Publication

Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PVS10

Plasmid

#104398

Purpose

polycistronic vector for expression of E. coli core RNAP

Depositor

Irina Artsimovitch

Publication

Svetlov et al Methods Mol Biol. 2015;1276:13-29. doi:

Insert

rpoA-rpoB-rpoC-6xHis and rpoZ (rpoA, rpoB, rpoC, rpoZ E. coli)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET28-his-3C_Tau352

Plasmid

#100092

Purpose

Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

Depositor

Nikolai Sluchanko

Publication

Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

Insert

Fetal microtubule-associated protein tau (MAPT Human)

Tags

His6

Expression

Bacterial

Mutation

wild type sequence

Promoter

T7

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9HF1-recA56

Plasmid

#89962

Purpose

Modified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Inserts

Cas9HF1
recA56

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet and proD

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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eSrtA(2A-9) pET29b

Plasmid

#75145

Purpose

reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

Depositor

David Liu

Publication

Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

reprogrammed sortase A variant

Tags

6x His

Expression

Bacterial

Mutation

S102C A104H E105D K138P K152I N160K K162H T164N K…

Promoter

T7

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBS-Int

Plasmid

#50000

Purpose

Expresses the integrase on a suicide plasmid

Depositor

Brian Robertson, Peter Sander

Publication

Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

Insert

Integrase from pMV361

Expression

Bacterial

Promoter

own

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles