32,967 results
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Plasmid#65616PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edCit-attR1 and attR2-edCer-cMycExpressionBacterialPromoterT7 promoterAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only
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pYTK073
Plasmid#65180PurposeEncodes ConRE' as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConRE'
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK076
Plasmid#65183PurposeEncodes HIS3 as a Type 6 part to be used in the Dueber YTK systemDepositorInsertHIS3
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK079
Plasmid#65186PurposeEncodes HygromycinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertHygromycinR
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK085
Plasmid#65192PurposeEncodes SpecR-ColE1 as a Type 8 part to be used in the Dueber YTK systemDepositorInsertSpecR-ColE1
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK091
Plasmid#65198PurposeEncodes SpecR-ColE1 as a Type 8a part to be used in the Dueber YTK systemDepositorInsertSpecR-ColE1
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-KA
Plasmid#64981PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108AAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL821-8
Plasmid#58224PurposeFor fungal transformation via ATMT. Transformants to be selected by hygromicin resistance.DepositorInserthygromycin B phosphotransferase
ExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGL822-8
Plasmid#58226PurposeFor fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.DepositorInsertphosphinothricin acetyl transferase
ExpressionBacterialPromoterAspergillus nidulans trpC promoterAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
4RFQ
Plasmid#64656PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL18:PBC004-E03:C231318 (METTL18 Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceMay 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
4RG1
Plasmid#64657PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertC9orf114:JMC099-C04:C232626 (SPOUT1 Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceMay 15, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRFP1-36
Plasmid#63825PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
ExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP1-1
Plasmid#63816PurposeA member of an RBS library for mRFP1 with different translation ratesDepositorInsertmRFP1
ExpressionBacterialMutationCodon optimized for E.coliPromoterJ23100Available SinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSET-A2-77p-Venus
Plasmid#64856PurposeExpresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tagDepositorInsertVWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Synthetic, Human)
Tags7X His and VenusExpressionBacterialPromoterT7 PromoterAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_5+7_gfp
Plasmid#60595Purposeencodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.DepositorInsertCas_5+7_gfp
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_2+5
Plasmid#60594Purposeencodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2DepositorInsertCas_2+5
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG 413
Plasmid#63859PurposeDetect translation initiation from CUG start codon (E. coli lacZ)DepositorAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Y203)
Plasmid#63559PurposeExpression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Y203)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged threonine at position 203 with respect to…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only