30,756 results

1. pHH0103_YWHAE_14-3-3

   Plasmid

   #109942

   Purpose

   Protein expression and purification of YWHAE_14-3-3

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   YWHAE_14-3-3
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX CblB-SH2

   Plasmid

   #46413

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   CblB (CBLB Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 42-359

   Promoter

   Tac

   Available Since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRRNAcos

   Plasmid

   #78492

   Purpose

   Transcription of Streptococcus pyogenes crRNA and tracrRNA under the wildtype crRNA promoter.

   Depositor

   David Bikard

   Article

   Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

   Insert

   tracrRNA
   

   Expression

   Bacterial

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. B6-3

   Plasmid

   #111487

   Purpose

   Ser-tRNA synthetase

   Depositor

   Cheemeng Tan

   Article

   Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

   Insert

   Ser-tRNA synthetase
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAP Z alpha 1 beta 1 CP

   Plasmid

   #13451

   Depositor

   John Cooper

   Article

   Soeno et al J Muscle Res Cell Motil. 1998 Aug . 19(6

   Insert

   capping protein alpha 1 beta 1 (chicken) (CAPZB Chicken)
   

   Expression

   Bacterial

   Mutation

   none

   Available Since

   Dec. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

6. MBP-hnRNPA2_LC

   Plasmid

   #98661

   Purpose

   Expresses MBP-tagged hnRNPA2 AA 190-341

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV:: HA-hM4Dnrxn

   Plasmid

   #52525

   Purpose

   Expresses N-terminal HA tagged hM4Dnrxn

   Depositor

   Scott Sternson

   Article

   Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

   Insert

   HA-hM4Dnrxn (CHRM4 Synthetic, Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15-EF4n_mFAP2b

   Plasmid

   #159464

   Purpose

   Encodes for EF4n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

   Depositor

   David Baker

   Insert

   EF4n_mFAP2b
   

   Tags

   6xHis-thrombin-TEV

   Expression

   Bacterial

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXS-mPlum

   Plasmid

   #117388

   Purpose

   pGEN-mcs with a modular mPlum expression scaffold

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Insert

   mPlum
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMT-ubb-Avi-Cerulean-Rpl10a

    Plasmid

    #79882

    Purpose

    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    

    Tags

    Avi

    Expression

    Bacterial

    Promoter

    Ubiquitin

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBEST-p15A-PLlacO1-deGFP-T500

    Plasmid

    #45394

    Depositor

    Vincent Noireaux

    Article

    Shin et al J Biol Eng. 2010 Jun 24;4:8.

    Insert

    deGFP
    

    Expression

    Bacterial

    Promoter

    PL-lacO1

    Available Since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ_3WJrep_N19_switch

    Plasmid

    #132723

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N19_switch
    

    Expression

    Bacterial

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDule-IBBN (G2)

    Plasmid

    #85500

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM263-EcPPK

    Plasmid

    #38333

    Depositor

    Florian Freimoser

    Insert

    E. coli polyphosphate kinase (ppk Escherichia coli)
    

    Tags

    6xHIS, GST, and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJ128

    Plasmid

    #19962

    Depositor

    Jonathan Tait

    Article

    Jin et al J Biol Chem 2004; 279:40351-57.

    Insert

    annexin V-128 (ANXA5 Human)
    

    Tags

    (Met)-Ala-Gly-Gly-Cys-Gly-His

    Expression

    Bacterial

    Mutation

    Deletion of the initiator Met at position 1 of wi…

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZE-P9-egfp

    Plasmid

    #201790

    Purpose

    the promoter can be recognized by PadR

    Depositor

    Yajun Yan

    Article

    Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

    Insert

    P9
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    July 11, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. Cas12c-sgRNA_BbsI_CAM

    Plasmid

    #183221

    Purpose

    Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRESpuro2-UBXD1-mCherry

    Plasmid

    #31835

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    UBXD1 (UBXN6 Human)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pUC-UAS1B16-Leum

    Plasmid

    #44324

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B16-Leum promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B16-Leum

    Available Since

    May 23, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pGex-3x MKK4

    Plasmid

    #14617

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    MKK4 (MAP2K4 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only