31,065 results

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pMT-ubb-Avi-Cerulean-Rpl10a

Plasmid

#79882

Purpose

Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)

Tags

Avi

Expression

Bacterial

Promoter

Ubiquitin

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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p15A-OptoCre-cat-T172A-P-R

Plasmid

#189854

Purpose

Cre-inducible activation of cat-T172A for chloramphenicol resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P-loxP-TT-loxP-R-catT172A

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P (T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Gi-c009

Plasmid

#173079

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Tags

mCitrine

Expression

Bacterial

Mutation

full length, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CoV-2-NSP3-c002

Plasmid

#173084

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))

Expression

Bacterial

Mutation

E1084-E1192, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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Stag-YCrs5

Plasmid

#215163

Purpose

Encodes a yeast cadmium metallothionein fused with the S-tag at the N-terminus

Depositor

Travis Beddoe, Bhuvana Shanbhag

Insert

Stag-YCrs5

Tags

S-tag

Expression

Bacterial

Promoter

T7 lac

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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mSNX13-PX (558-677)

Plasmid

#119094

Purpose

Bacterial expression of human phox homology (PX) domain, mSNX13-PX (558-677)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

mSNX13-PX (558-677)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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SNX14-PX (561-686)

Plasmid

#119095

Purpose

Bacterial expression of human phox homology (PX) domain, SNX14-PX (561-686)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX14-PX (561-686) (SNX14 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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NUDT4 (NUDT4A-c001)

Plasmid

#98241

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

NUDT4A (NUDT4 Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX2T E7 Q

Plasmid

#13648

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 Q mutant (E7 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 30-37 changed from DSSEEEDE to QSSQQQQQ.

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pLpp5oGFP

Plasmid

#69345

Purpose

Orthogonal GFP message under lpp5 promoter

Depositor

Michael Jewett

Article

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

super folder GFP

Expression

Bacterial

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-Leum

Plasmid

#44324

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-Leum

Available Since

May 23, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pCoofy61

Plasmid

#122013

Purpose

E.coli vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

Depositor

Peggy Stolt-Bergner, Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-eGFP

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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sfTq2-PBP5

Plasmid

#117959

Purpose

Periplasmic expression of superfolder mTurquoise2 (pNM067 pTHV037-DsbAss-LEGPAGL-sfTq2-EFGS-∆1-17-PBP5)

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

sfTq2-PBP5 (dacA )

Expression

Bacterial

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP3

Plasmid

#80659

Purpose

Encodes the synthetic coiled coil peptide SYNZIP3

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP3

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-O-GECO1

Plasmid

#113938

Purpose

Bacterial expression of orange fluorescent Ca2+ sensor O-GECO1

Depositor

Robert Campbell, Thomas Hughes

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

O-GECO1

Tags

6xHis

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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mCherryS131C

Plasmid

#48732

Purpose

Expresses mCherryS131C in E. coli cells

Depositor

Bradley Olsen

Article

Thomas et al ACS Nano. 2011 Jul 26;5(7):5697-707. doi

Insert

mCherryS131C

Tags

6xHis

Expression

Bacterial

Mutation

changed serine 131 to cysteine

Promoter

T5

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pBSSK mPTP-1B

Plasmid

#8603

Depositor

Ben Neel

Article

Chernoff et al Proc Natl Acad Sci U S A 1990 Apr;87(7):

Insert

PTP-1B (Ptpn1 Mouse)

Expression

Bacterial

Available Since

June 21, 2005

Availability

Academic Institutions and Nonprofits only
pETM33_Nsp3b_ADRP

Plasmid

#156468

Purpose

Bacterial expression of Sars-CoV2 Nsp3b_ADRP protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3b_ADRP (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ChmN

Plasmid

#221348

Purpose

Express ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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Pxyl-zapT-mScarlet-I

Plasmid

#207249

Purpose

Integrative plasmid for expressing ZapT-mScarlet-I in Caulobacter crescentus CB15N under Pxyl promoter

Depositor

Suliana Manley

Article

Zhang et al Nat Commun. 2024 Apr 24;15(1):3460. doi:

Insert

ZapT-mScarlet-I

Tags

mScarlet-I

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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