30,756 results

1. pSiMPlh_N + pSiMPlh_C

   Plasmid

   #134316

   Purpose

   Maintenance of 2 plasmids with a single antibiotic in E. coli

   Depositor

   Barbara Di Ventura

   Article

   Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

   Insert

   hygR_gp41-1 N-intein + hygR_gp41-1 C-intein
   

   Expression

   Bacterial

   Mutation

   P254Q in hygR_gp41-1 N-intein

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. p405CYC1

   Plasmid

   #15967

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   p405CYC1 (CYC1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   CYC1 promoter is between SacI and XbaI restrictio…

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-EGFAB(H306Y)

   Plasmid

   #25771

   Depositor

   Johann Deisenhofer

   Article

   McNutt et al J Biol Chem. 2009 Apr 17. 284(16):10561-

   Insert

   LDL receptor EGFAB (LDLR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   His 306 to Tyr

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pJB066

   Plasmid

   #98913

   Purpose

   Express FtsZ tagged with pAmCherry1 in E coli

   Depositor

   Jie Xiao

   Article

   Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

   Insert

   FtsZ
   

   Tags

   pAmCherry1

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKM343

   Plasmid

   #71487

   Purpose

   Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.

   Depositor

   Kenan Murphy

   Article

   Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

   Insert

   loxP-hyg-cat-loxP
   

   Use

   Source of loxp-hyg-cat- loxp for pcr amplificatio…

   Expression

   Bacterial

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMAL658

   Plasmid

   #167837

   Purpose

   Encodes OptoLAC1B circuit

   Depositor

   Jose Avalos

   Article

   Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

   Insert

   lacI
   

   Use

   Synthetic Biology

   Tags

   ssrA (LAA)

   Expression

   Bacterial

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMAL659

   Plasmid

   #167838

   Purpose

   Encodes OptoLAC2B circuit

   Depositor

   Jose Avalos

   Article

   Lalwani et al Nat Chem Biol. 2021 Jan;17(1):71-79. doi

   Insert

   lacI
   

   Use

   Synthetic Biology

   Tags

   ssrA (AAV)

   Expression

   Bacterial

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-SUMO mCER-Prx2

   Plasmid

   #158226

   Purpose

   Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (Recombinant bacterial expression)

   Depositor

   Tobias Dick

   Article

   Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

   Insert

   mCER-Prx2
   

   Expression

   Bacterial

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMTB2-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80067

   Purpose

   Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. PC4_3spc34h

    Plasmid

    #70700

    Purpose

    Expresses S. cerevisiae DASH/Dam1 complex with C-terminal his-tag on spc34

    Depositor

    Stephen Harrison

    Article

    Miranda et al Nat Struct Mol Biol. 2005 Feb;12(2):138-

    Insert

    DASH / Dam1 complex (DAM1, ASK1, DUO1, SPC34, SPC19, DAD1, DAD2, DAD4, HSK3, DAD3 Budding Yeast)
    

    Tags

    his-tag on C-terminus of spc34

    Expression

    Bacterial

    Available Since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. UBH12 (3BI7)

    Plasmid

    #25219

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ubh12.414.617 (UHRF1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    SRA Domain: Residues 414-617

    Available Since

    Aug. 12, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pGMCSq17-10M-0X

    Plasmid

    #27364

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    tetR(sB)
    

    Use

    Gateway destination vector

    Expression

    Bacterial

    Mutation

    N/A

    Available Since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSFDuet-1 IntS11 AA 300-597

    Plasmid

    #199329

    Purpose

    Expresses His-tagged Drosophila IntS11 amino acids 300-597

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 11 (IntS11 Fly)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 300-597 only

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBad33_ISMmu1_reRNA

    Plasmid

    #205349

    Purpose

    Bacterial expression of ISMmu1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISMmu1 TnpB
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAJT254-litR

    Plasmid

    #202308

    Purpose

    litR repressor plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    litR
    

    Expression

    Bacterial

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAJT249-psrA

    Plasmid

    #202304

    Purpose

    psrA repressor plasmid for LPattB2 integration

    Depositor

    Christopher Voigt

    Article

    Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

    Insert

    psrA
    

    Expression

    Bacterial

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15-EF1p3_mFAP2b

    Plasmid

    #159470

    Purpose

    Encodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    EF1p3_mFAP2b
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15-mFAP10

    Plasmid

    #159463

    Purpose

    Encodes for mFAP10 with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

    Depositor

    David Baker

    Insert

    mFAP10
    

    Tags

    6xHis-thrombin-TEV

    Expression

    Bacterial

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBSSK mPTP-1B

    Plasmid

    #8603

    Depositor

    Ben Neel

    Article

    Chernoff et al Proc Natl Acad Sci U S A 1990 Apr;87(7):

    Insert

    PTP-1B (Ptpn1 Mouse)
    

    Expression

    Bacterial

    Available Since

    June 21, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pSB_init_Sb92

    Plasmid

    #159424

    Purpose

    Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    Sb92
    

    Tags

    c-Myc and poly-His

    Expression

    Bacterial

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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