30,753 results

1. ACS2 Truncated

   Plasmid

   #13748

   Depositor

   John Denu

   Article

   Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

   Insert

   Truncated Acetyl CoA Sythetase 2 (Acss1 Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 39–682

   Available Since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

2. bbCas9pluspAAA

   Plasmid

   #82581

   Purpose

   The plasmid encodes the T7 promoter, Cas9 (from pX330 #42230) and a stretch of poly-A. After linearization with restriction enzyme SapI It is used to produce in vitro transcribed mRNA

   Depositor

   Ivo Huijbers

   Article

   Pritchard et al Methods Mol Biol. 2017;1642:21-35. doi:

   Insert

   Cas9
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. C-BLa 2.0

   Plasmid

   #92060

   Purpose

   The C-BLa 2.0 plasmid encodes a protein, where the TMD is connected to the C-terminus of the cytoplasmic domain of the ToxR protein and to the N-terminus of the C-terminal beta-lactamase fragment.

   Depositor

   Dieter Langosch

   Article

   Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

   Insert

   C-BLa 2.0 fusion protein
   

   Tags

   Flag (DYKDHDG)

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRB-polySH33-EGFP

   Plasmid

   #175792

   Purpose

   Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRB-polySH35

   Plasmid

   #175234

   Purpose

   Bacterial expression and purification, mixed with polyPRM to form condensates, can bind FKBP in the presence of rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX4T1-Smurf1 C699A

   Plasmid

   #13503

   Depositor

   Jeff Wrana

   Article

   Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

   Insert

   SMURF1 (SMURF1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C699A: catalytically inactive

   Available Since

   Dec. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pTYB2-PAB1

   Plasmid

   #37234

   Depositor

   Jon Lorsch

   Article

   Park et al EMBO J. 2011 Jan 19;30(2):302-16. Epub 2

   Insert

   PAB1 (PAB1 Budding Yeast)
   

   Tags

   intein

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

8. 6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)

   Plasmid

   #169168

   Purpose

   Conjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Tags

   6X Histidine Tag, TEV cleavage site, mCherry

   Expression

   Bacterial

   Promoter

   T7 lac promoter

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGSTag Flag Jnk3a2

   Plasmid

   #15748

   Depositor

   Roger Davis

   Article

   Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

   Insert

   Jnk3a2 (MAPK10 Human)
   

   Expression

   Bacterial

   Available Since

   March 6, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pGEX-6P-2_dCrk

    Plasmid

    #131146

    Purpose

    Expresses GST::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. AHCYL1A

    Plasmid

    #42465

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AHCYL1A (AHCYL1 Human)
    

    Tags

    His6-StrepII-TEV

    Expression

    Bacterial

    Mutation

    See Comments

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pET28a-pro-sumo3

    Plasmid

    #25103

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO3 (SUMO3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Serine 2 to Alanine

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pBT-4

    Plasmid

    #22831

    Depositor

    Ryan Gill

    Article

    Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-GAR

    Plasmid

    #34697

    Depositor

    Steven Clarke

    Article

    Frankel et al J Biol Chem. 2000 Oct 20;275(42):32974-8

    Insert

    Fibrillarin (FBL Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 1-145 of human fibrillarin with K2E and …

    Promoter

    tac

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCellFree_G06_ZIKV NS3

    Plasmid

    #145355

    Purpose

    Cell-free expression vector for Zika virus NS3 protein with C-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS3
    

    Tags

    mCherry and 8xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHH0103_GNA11_G-alpha

    Plasmid

    #109911

    Purpose

    Protein expression and purification of GNA11_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNA11_G-alpha (GNA11 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDOC-K-glmS-mPlum

    Plasmid

    #158062

    Purpose

    Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    monomeric plum fluoresecent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    acpP

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOSV812

    Plasmid

    #126605

    Purpose

    modular integrative vector for the assembly of DNA fragments and expression in Streptomyces species

    Depositor

    Jean-Luc Pernodet

    Article

    Aubry et al Appl Environ Microbiol. 2019 Jun 7. pii:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM-T Dmel Or35a

    Plasmid

    #59650

    Purpose

    Probe for Drosophila melanogaster Or35a expression

    Depositor

    Richard Axel, Leslie Vosshall

    Article

    Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

    Insert

    Or35a (Or35a Fly)
    

    Expression

    Bacterial

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pTCF3 H-2D(d)

    Plasmid

    #180452

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    H-2D(d)
    

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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