30,744 results

1. pdCas9-AsiA

   Plasmid

   #158063

   Purpose

   Expressing CasTA of dCas9 fused AsiA

   Depositor

   Harris Wang

   Article

   Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

   Insert

   dCas9-AsiA
   

   Expression

   Bacterial

   Promoter

   TetO

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CBLB

   Plasmid

   #39056

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CBLB (CBLB Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pVRb22_up1147

   Plasmid

   #49716

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   sfgfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Pecf22_up1147

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDC184

   Plasmid

   #70055

   Purpose

   Co-expresses Syt1-SNARE37aa-linker complex with p045

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

   Inserts

   His tagged Synaptobrevin 28-89
   Syntaxin 191-256
   

   Tags

   10xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. NAT2

   Plasmid

   #25182

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NAT2_07 (NAT2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 9, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pBbB3k-GFP

   Plasmid

   #35348

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   ProS

   Available Since

   Feb. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS Wnt-1HA

   Plasmid

   #17367

   Depositor

   Jan Kitajewski

   Article

   Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

   Insert

   Wnt-1HA (Wnt1 Mouse)
   

   Tags

   HA

   Expression

   Bacterial

   Available Since

   July 7, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-MSP1D1deltaH4/2

   Plasmid

   #71712

   Purpose

   Membrane scaffold protein (MSP), half of helix 4 truncated

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Available Since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4046810

   Plasmid

   #149251

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   May 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGEX-4T_CIP2A_1-560

    Plasmid

    #217921

    Purpose

    E. coli expression of GST-tagged CIP2A_1-560 protein

    Depositor

    Jukka Westermarck

    Insert

    CIP2A (CIP2A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSR241

    Plasmid

    #125091

    Purpose

    Expression of ompR(1-137aa)-yxdJ(132-229aa) chimera under PLtetO-1, output Promoter PyxdL181

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    ompR(REC)-yxdJ(DBD)137
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. UCP3 promoter EP1

    Plasmid

    #71743

    Purpose

    Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activity

    Depositor

    Heinrich Taegtmeyer

    Article

    Harmancey et al J Biol Chem. 2015 Nov 10. pii: jbc.M115.

    Insert

    Uncoupling Protein 3 promoter (Ucp3 Mouse)
    

    Tags

    Luciferase

    Expression

    Bacterial

    Mutation

    Contains 7kb of the murine UCP3 gene encompassing…

    Promoter

    UCP3

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDHK29

    Plasmid

    #80783

    Purpose

    Cloning vector compatible with ColE1 plasmids with high copy number

    Depositor

    Gregory Phillips

    Article

    Phillips et al Biotechniques. 2000 Mar;28(3):400-2, 404

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTn7·xylS

    Plasmid

    #122591

    Purpose

    Plasmid for genomic integraction of the xylS regulator into the Tn7 insertion site

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    xylS
    

    Use

    Synthetic Biology; Tn7 genomic integration

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5FRT/TO-p97-EQ-mycStrep

    Plasmid

    #31838

    Depositor

    Hemmo Meyer

    Article

    Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

    Insert

    p97
    

    Tags

    Strep and myc

    Expression

    Bacterial and Mammalian

    Mutation

    E578Q

    Promoter

    CMV

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pGEX p85a(C)-SH2

    Plasmid

    #46463

    Depositor

    Bruce Mayer, Leszek Kotula

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    p85a(C) (PIK3R1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 612-724

    Promoter

    Tac

    Available Since

    July 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15-MTFmt

    Plasmid

    #31174

    Depositor

    Linda Spremulli

    Article

    Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

    Insert

    Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
    

    Tags

    His and thrombin cleavage

    Expression

    Bacterial

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pET30a-SARS-CoV2-ORF3b (S23Q)

    Plasmid

    #165088

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
    

    Expression

    Bacterial

    Mutation

    Stop codon to glutamine at 23rd amino acid

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pOPS0359

    Plasmid

    #115508

    Purpose

    Reporter Plasmid. Taurine-inducible expression of GFP

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Taurine-inducible promoter of S. meliloti Promote…

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQTEV-TPT1

    Plasmid

    #31558

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    tumor protein, translationally-controlled 1 (TPT1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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