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K401-iLid

Plasmid

#122484

Purpose

Component of optically-induced motor dimerization system

Depositor

Matt Thomson

Article

Ross et al Nature. 2019 Aug;572(7768):224-229. doi:

Insert

Kinesin 1-401 iLid His tag

Tags

His6 and iLid

Expression

Bacterial

Mutation

Kinesin amino acids 1-401

Promoter

T7

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

Plasmid

#99221

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.1

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_nocharge

Plasmid

#139118

Purpose

express hnRNPA2 LC with no charged residues

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all charged residues are changed to S/Q except fo…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pExp-PpiB

Plasmid

#112572

Purpose

Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

Depositor

Marko Hyvönen

Type

Empty backbone

Tags

8xHis-PpiB and Strep tag

Expression

Bacterial

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b I-SceI (Ctermhis -MH)

Plasmid

#195613

Purpose

Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

Depositor

Frederick Gimble

Article

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mEGFPBb

Plasmid

#118234

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mEGFP

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1K3_pBen-sfGFP_J23101-mRFP

Plasmid

#128126

Purpose

Expressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pBen (benzoate responsive promoter)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET11b-SpyCatcher-M2e

Plasmid

#165990

Purpose

SpyCatcher-fused influenza M2 ectodomain with removable HisTag

Depositor

Vesa Hytönen

Article

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

SpyCatcher-M2e

Tags

HisTag and SpyCatcher

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBsVchCAST

Plasmid

#203812

Purpose

Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAM5080

Plasmid

#85106

Purpose

Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

YFP-KaiC

Expression

Bacterial

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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BASIC_11_RBS34-GFP-B0015

Plasmid

#68145

Purpose

storage vector for BASIC part: RBS-GFP(ORF)

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

RBS-34-GFP-B0015

Expression

Bacterial

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1

Plasmid

#154189

Purpose

To express LanCL1 in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Tags

Hexahistidine

Expression

Bacterial

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pNAND-PhlF

Plasmid

#49376

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

PhlF repressor, pPhlF-pLmrA reporter

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSkunk3-BLA

Plasmid

#61531

Purpose

Phagemid expressing streptomycin/spectinomycin resistance gene

Depositor

Marc Ostermeier

Article

Firnberg et al PLoS One. 2012;7(12):e52031. doi: 10.137

Insert

aadA

Expression

Bacterial

Promoter

lacI

Available Since

Dec. 12, 2014

Availability

Academic Institutions and Nonprofits only

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p1977 (pUC19-PTDH3-PTEF1)

Plasmid

#78230

Purpose

Example plasmid containing a bidirectional promoter of pTDH3 and pTEF1

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

Bidirectional promoters pTDH3-pTEF1

Expression

Bacterial

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only
pQLinkHD-SYNZIP4

Plasmid

#80660

Purpose

Encodes the synthetic coiled coil peptide SYNZIP4

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP4

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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TOPO rtTA3-2A-Cre

Plasmid

#68448

Purpose

TOPO construct expressing rtTA3-2A-Cre for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

rtTA3-2A-Cre

Use

Gmap

Expression

Bacterial

Mutation

Codon optimized for expression in mice

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

Plasmid

#204794

Purpose

Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

West Nile NS2B-NS3 fusion

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

Plasmid

#198599

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-143-144

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp8

Plasmid

#156477

Purpose

Bacterial expression of Sars-CoV2 Nsp8 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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