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34,150 results

Showing: 1101 - 1120 of 34150 results

  6. pSL0284 (pQCascade_entry)

    Plasmid
    #130635
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.
    Depositor
    Samuel H. Sternberg
    Publication
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    130635-plasmid-map-sequence-id-255022

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  10. pALOD4

    Plasmid
    #111026
    Purpose
    Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A
    Depositor
    Arun Radhakrishnan
    Publication
    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d
    Insert
    Domain 4 of Anthrolysin O
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Mutations: S404C, C472A
    Promoter
    T7
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111026-plasmid-map-sequence-id-214044

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  11. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Publication
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86846-plasmid-map-sequence-id-172845

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  12. pFA6a-HaloTag-KanMX6

    Plasmid
    #87029
    Purpose
    Tag S. pombe genes in C terminal with HaloTag
    Depositor
    Julien Berro
    Insert
    HaloTag
    Expression
    Bacterial and Yeast
    Available since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87029-plasmid-map-sequence-id-173001

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  14. pET28-Dnmt3a3L-sc27

    Plasmid
    #71827
    Purpose
    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.
    Depositor
    Albert Jeltsch
    Publication
    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do
    Insert
    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    Tags
    6xHis tag
    Expression
    Bacterial
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71827-plasmid-map-sequence-id-254300

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Showing: 1101 - 1120 of 34150 results