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SupD v2

Plasmid

#198598

Purpose

Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SupD

Expression

Bacterial

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTA-Mob 2.0 Tp

Plasmid

#188619

Purpose

Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Conjugation helper plasmid

Expression

Bacterial and Yeast

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pJV62

Plasmid

#26910

Depositor

Graham Hatfull

Article

van Kessel et al Mol Microbiol. 2008 Mar . 67(5):1094-107

Insert

Che9c gene 61

Expression

Bacterial

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pQE60 R124A wgMDH

Plasmid

#204257

Purpose

Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R124A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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BPK2101

Plasmid

#65770

Purpose

Bacterial expression plasmid for S. aureus Cas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSaCas9-NLS-3xFLAG-T7-BsaIcassette-Sa-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized SaCas9, and SaCas9 gRNA

Use

CRISPR

Tags

NLS-3xFlag

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pINTO_7/8

Plasmid

#206431

Purpose

A vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTSA-13

Plasmid

#17327

Depositor

Takeshi Sano

Article

Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

Insert

streptavidin

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Available Since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pSHDY-Prha-mVenus_rhaS

Plasmid

#137662

Purpose

Conjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.

Depositor

Ilka Axmann

Article

Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

Insert

Prha:mVenus

Expression

Bacterial

Mutation

rhaS (Arg218Leu)

Promoter

Prha

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2018

Plasmid

#140665

Purpose

dCas9, LuxR & Csy4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9, LuxR & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor T19N

Plasmid

#12152

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor T19N (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA T19N (dominant-negative mutation)*

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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