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Bacterial Expression

30,744 results

Showing: 11181 - 11200 of 30744 results

  2. pGBW-m4046942

    Plasmid
    #145757
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145757-plasmid-map-sequence-id-292503

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  3. pSL0747 (pDonor_L-MmeI)

    Plasmid
    #130647
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated left end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (L*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATG --> TGTTGGAG
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130647-plasmid-map-sequence-id-255647

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  4. pORTMAGE502B

    Plasmid
    #128971
    Purpose
    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    xyl-Pm
    Available Since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    128971-plasmid-map-sequence-id-252654

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  5. USP08 (3N3K)

    Plasmid
    #28115
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP08 (USP8 Human)
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    Catalytic domain only.
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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  16. pUS252s

    Plasmid
    #191833
    Purpose
    Expresses fuGFPs constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPs to place in other plasmid backbones
    Depositor
    Nicholas Coleman
    Insert
    fuGFPs
    Expression
    Bacterial
    Available Since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191833-plasmid-map-sequence-id-382124

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  18. CUL3A-c012

    Plasmid
    #53673
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    CUL3A
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Available Since
    June 13, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 11181 - 11200 of 30744 results