33,480 results
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Plasmid#215769Purposeencodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locusDepositorInsertsExpressionBacterialMutationCas12a PAM mutatedAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only
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pDonor-LoxP
Plasmid#219436PurposeCRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegensDepositorInserttwo transposon ends, LoxP-terminator-LoxP gene cassette
ExpressionBacterialAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSIBR4-Cre
Plasmid#219435PurposeTheophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinaseDepositorInsertintron 4-Cre
ExpressionBacterialAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAGD236
Plasmid#219497PurposepCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoterDepositorInsertsfGFP, Eco1 msr, Eco1 RT
ExpressionBacterialPromoterT7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
PARP2-c098
Plasmid#216873PurposeBacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)
ExpressionBacterialAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-CaPETaseM9
Plasmid#218707PurposeEncodes codon optimized CaPETaseM9 enzyme for bacterial expressionDepositorInsertCaPETaseM9
Tags10xHisExpressionBacterialAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptrc99a-pVhb-tdTER-fdh
Plasmid#215649PurposepBR322 Origin, AmpR, with tdTER and Fdh genes under Vhb promoterDepositorInsertstrans-enoyl-CoA reductase
formate dehydrogenase
ExpressionBacterialPromoterVhbAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMVV205
Plasmid#215342PurposeRecombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backboneDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23105Available sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-TfCut2-S121P-D174S-D204P
Plasmid#218695PurposeEncodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expressionDepositorInsertTfCut2
Tags10xHisExpressionBacterialMutationS121P-D174S-D204PAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-BhrPETase
Plasmid#218692PurposeEncodes codon optimized BhrPETase enzyme for bacterial expressionDepositorInsertBhrPETase
Tags10xHisExpressionBacterialAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(CTLA4)
Plasmid#177905PurposeBacterial expression of his10-tagged human CTLA4 Intracellular DomainDepositorInsertCTLA4 (aa 183-223 only) (CTLA4 Human)
Tags10xHis-Thrombin cut site-T7 tagExpressionBacterialPromoterT7Available sinceMay 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cut190*_pET21b
Plasmid#215404PurposeExpression construct for Cut190* gene, codon optimized for expression in E. coliDepositorInsertCut190*
ExpressionBacterialMutationS226P/R228SAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG-HF1-Hypa
Plasmid#218166PurposeThis plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG-HF1-Hypa
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2n-6xHis
Plasmid#218886PurposeBacterial expression of improved GABA sensor (negative change in fluorescence)DepositorInsertiGABASnFR2n
Tags6xHisExpressionBacterialMutationS99A F104H R168PPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA333
Plasmid#214745PurposeMedium copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
ExpressionBacterialAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
peSCBE3-NG
Plasmid#218163PurposeThis plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.DepositorInsertsescbe3-NG
a programmable sgRNA cloning cassette
UseCRISPR; Sgrna transcriptionExpressionBacterialMutationMutation in the portion of deaminase hAPOBEC3A : …PromoterrpsL promoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT22
Plasmid#200996PurposepLv2-pUC18R6KT-miniTn7-Km, KmR, ApRDepositorTypeEmpty backboneExpressionBacterialAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT13.G
Plasmid#201001PurposepLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApRDepositorInsertsuperfolder GFP
ExpressionBacterialPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT21.RY
Plasmid#201006PurposepLv1-pUC18R6KT-miniTn7-Gm-mCherry-sYFP2, GmR, ApRDepositorInsertsmcherry
sYFP2
ExpressionBacterialPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT21.YB
Plasmid#201008PurposepLv1-pUC18R6KT-miniTn7-Gm-sYFP2-TagBFP, GmR, ApRDepositorInsertssYFP2
TagBFP
ExpressionBacterialPromoterJ23104Available sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only