30,735 results

1. RBM15B_3xRRM_pGEX

   Plasmid

   #135125

   Purpose

   Recombinant expression of RBM15B with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   RBM15B (RBM15B Human)
   

   Tags

   GST-SBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLIC-SGC1

   Plasmid

   #39187

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Nicola Burgess-Brown

   Type

   Empty backbone

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT7-7 asyn S129G

   Plasmid

   #36049

   Depositor

   Hilal Lashuel

   Article

   Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

   Insert

   alpha-synuclein S129G (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   S129G

   Promoter

   T7

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ZP36 Pax2

   Plasmid

   #16906

   Depositor

   Randall Moon

   Insert

   pax2 (pax2a Zebrafish)
   

   Expression

   Bacterial

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pAB088c

   Plasmid

   #79213

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI-TnTBR3-F3
   

   Expression

   Bacterial

   Promoter

   PlacZ-opt (OR1); Ppro1

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103_ARHGAP32-1/1

   Plasmid

   #91312

   Purpose

   Protein expression and purification of human SH3 domain construct ARHGAP32-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ARHGAP32-1/1 (ARHGAP32 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28-mTDGa.1

   Plasmid

   #81051

   Purpose

   bacterial expression of catalytically dead murine TDG

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TDG (Tdg Mouse)
   

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   asparagine 151 changed to alanine

   Promoter

   T7

   Available Since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Pr-deGFP-MGapt

   Plasmid

   #67734

   Purpose

   OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

   Depositor

   Richard Murray

   Article

   Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

   Insert

   UTR1-deGFP-MGapt-T500
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHH0103_GNAT3_G-alpha

   Plasmid

   #109948

   Purpose

   Protein expression and purification of GNAT3_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNAT3_G-alpha (GNAT3 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSP64T tBMPR (DM#135)

    Plasmid

    #15067

    Depositor

    Douglas Melton

    Article

    Graff et al Cell. 1994 Oct 7. 79(1):169-79.

    Insert

    BMP receptor (bmpr1a Frog)
    

    Expression

    Bacterial

    Mutation

    Truncated dominant negative form

    Available Since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pYTK084

    Plasmid

    #65191

    Purpose

    Encodes KanR-ColE1 as a Type 8 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    KanR-ColE1
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCoofy41

    Plasmid

    #55184

    Purpose

    Insect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…

    Expression

    Bacterial

    Promoter

    pPH (Polyhedrin promoter)

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p7XC3GH

    Plasmid

    #47066

    Purpose

    FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Eric Geertsma, Raimund Dutzler

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMTP130 (pTA106_TnsABC(Tn6900))

    Plasmid

    #164261

    Purpose

    Vector expressing the TnsA, TnsB, and TnsC transposition proteins from the Tn6900 derivative element with a lactose promoter.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Insert

    tnsA, tnsB, and tnsC from Tn6900 derivative from A. salmonicida S44 pS44-1
    

    Expression

    Bacterial

    Mutation

    (tnsA) Changed Alanine 125 to Aspartic acid

    Promoter

    Lactose

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM33_Nsp3a_Ub1

    Plasmid

    #156467

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3a_Ub1 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3a_Ub1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p_SNIPR_target_42_mut_G

    Plasmid

    #139467

    Purpose

    T7 RNAP-driven expression of WT trigger RNA with mutation at 42

    Depositor

    Alexander Green

    Article

    Hong et al Cell. 2020 Feb 25. pii: S0092-8674(20)30

    Insert

    target_42_mut_G
    

    Expression

    Bacterial

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX 4T-1 GST-Ago2 S387A

    Plasmid

    #60656

    Purpose

    Expresses GST-Ago2 S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    S387A

    Promoter

    tac

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FEVA-c012

    Plasmid

    #53666

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    FEVA
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pGBW-m4046933

    Plasmid

    #145592

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge