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UBH12 (3BI7)

Plasmid

#25219

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

ubh12.414.617 (UHRF1 Human)

Tags

His

Expression

Bacterial

Mutation

SRA Domain: Residues 414-617

Available Since

Aug. 12, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJEC625

Plasmid

#174368

Purpose

Golden gate vector for gRNA cloning. Contains BbsI sites for insertion of the gRNA spacer as annealed oligos.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDJSVT90

Plasmid

#109304

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

TamA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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SRS-MTBD(22/19), AB(monomer), wt

Plasmid

#22380

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

His and myc

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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B5-2

Plasmid

#111481

Purpose

Arg-tRNA synthetase

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Arg-tRNA synthetase

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Pk-SOD1

Plasmid

#25574

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

Pk-SOD1

Tags

His

Expression

Bacterial

Available Since

Sept. 10, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pETM41-EcPPXc

Plasmid

#38329

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available Since

Aug. 6, 2012

Availability

Academic Institutions and Nonprofits only
pEE077

Plasmid

#176834

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

716

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPlacPbuCas13b-gRNA-T-MS2

Plasmid

#184839

Purpose

Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119 and lac promoter

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_LYN-1/1

Plasmid

#91536

Purpose

Protein expression and purification of human SH3 domain construct LYN-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LYN-1/1 (LYN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFLIPmal-40uDelta1YFP

Plasmid

#18853

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Insert

optimized maltose FRET sensor

Tags

CFP and YFP

Expression

Bacterial

Mutation

N227A;

Available Since

Oct. 23, 2008

Availability

Academic Institutions and Nonprofits only
pBT-3

Plasmid

#22832

Depositor

Ryan Gill

Article

Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

Type

Empty backbone

Expression

Bacterial

Available Since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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pZE-P9-egfp

Plasmid

#201790

Purpose

the promoter can be recognized by PadR

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

P9

Expression

Bacterial

Mutation

WT

Available Since

July 11, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pSKA397

Plasmid

#80380

Purpose

mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

Depositor

Mustafa Khammash

Article

Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

Inserts

cat
metE

Use

Synthetic Biology; Mini-tn7 delivery vector

Expression

Bacterial

Promoter

cat promoter and cpcG2Δ59

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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C2-66 pComb3xss anti-cFos nanobody

Plasmid

#145833

Purpose

Bacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-cFos (Human) recombinant llama nanobody C2-66 (FOS L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC-TolB-NHSTIIT-GG

Plasmid

#215792

Purpose

Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pOPINO_F2

Plasmid

#171926

Purpose

Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2

Depositor

Ray Owens

Article

Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:

Insert

VHH_F2

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Available Since

Sept. 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSK+ mBMPR-1B DN

Plasmid

#49530

Purpose

cloning vector for dominant negative mouse BMP receptor 1B

Depositor

Lee Niswander, Peter ten Dijke

Article

Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

Insert

BMPR-1B DN (Bmpr1b Mouse)

Expression

Bacterial

Mutation

dominant negative mutation (K231R); also L2V

Promoter

T3

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only