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pBAD02_CBASS_Escherichia_coli_BWH59

Plasmid

#224393

Purpose

For expression of the type I CBASS system encoded by Escherichia coli BWH59

Depositor

Philip Kranzusch

Publication

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

2TM_EcCdnD

Expression

Bacterial

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYTSK01K_0G7

Plasmid

#177296

Purpose

genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

Depositor

Karl-Erich Jaeger

Publication

Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

Insert

PaacC1-aacC1

Use

Synthetic Biology; Yeast expression, tn7 genomic …

Expression

Bacterial

Available since

Aug. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-TEX264_wt-His6

Plasmid

#220214

Purpose

Bacterial expression of wild type TEX264, C-terminal His6 tag

Depositor

Kristijan Ramadan

Publication

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-6p1-GST-Ubiquitin

Plasmid

#223236

Purpose

Bacterial expression construct for GST-Tagged Ubiquitin production and purification

Depositor

Weixing Zhao

Insert

Ubiquitin

Expression

Bacterial

Promoter

tac

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyB-GFP

Plasmid

#220190

Purpose

sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC-GFP

Plasmid

#220191

Purpose

sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter with GFP in sgRNA site

Use

CRISPR

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-LacI-dCas9

Plasmid

#220194

Purpose

mini-Tn7 dCas9 expression vector - LacI/pLac promoter

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

LacI/pLac

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET41a iGluh

Plasmid

#119830

Purpose

Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Publication

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluh

Tags

GST

Expression

Bacterial

Mutation

E25A

Promoter

T7

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SoxS-GFP

Plasmid

#220165

Purpose

To track the natural SoxS promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Publication

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

SoxS promoter only

Expression

Bacterial

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-D-TOPO-PARP14-SΔK

Plasmid

#216658

Purpose

Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

Depositor

Herwig Schüler

Publication

Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

Insert

PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Synthetic, Human)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

Deletion starting from Q1390 to D1448 and substit…

Promoter

T7

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRSET mNG-GECO1

Plasmid

#201933

Purpose

Expresses mNG-GECO1 indicator in bacteria

Depositor

Robert Campbell

Publication

Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

Insert

mNeonGreenGECO1

Tags

HIS tag

Expression

Bacterial

Available since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-FGFR1

Plasmid

#222611

Purpose

Expression of MAC3-tagged FGFR1

Depositor

Markku Varjosalo

Publication

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FGFR1 (FGFR1 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MAC3-EGFR

Plasmid

#222610

Purpose

Expression of MAC3-tagged EGFR

Depositor

Markku Varjosalo

Publication

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFR (EGFR Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MUNC18a

Plasmid

#218888

Purpose

Expresses rat MUNC18a in bacteria

Depositor

Axel Brunger

Publication

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

Munc18a (Stxbp1 Rat)

Tags

6 his

Expression

Bacterial

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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RepairTemplate_NIPBL_mEGFP_mAID

Plasmid

#217664

Purpose

Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

Depositor

Daniel Gerlich

Publication

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NIPBL_mEGFP_mAID (NIPBL Human)

Tags

mAID and meGFP

Expression

Bacterial and Mammalian

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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His_CL7_2NLS_iCas12a-4NLS

Plasmid

#222971

Purpose

Bacterial expression of NLS-rich iCas12a construct

Depositor

Jennifer Doudna

Publication

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His_CL7_2NLS_iCas12a-4NLS

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMVV098

Plasmid

#215304

Purpose

Expresses sfGFP under induction with the DAPG Marionette Cassette (PhIFAM + PPhIF-sfGFP) with a BBR1 backbone.

Depositor

Brian Pfleger

Publication

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Use

Synthetic Biology

Tags

None

Expression

Bacterial

Promoter

PPhIF

Available since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3079 (p7INT-Pveg_lacI)

Plasmid

#218505

Purpose

integrative plasmid enabling constitutive expression of lacI in Streptococcus, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

Depositor

Emmanuelle Charpentier

Publication

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

lacI (lacI Escherichia coli)

Expression

Bacterial

Promoter

Pveg

Available since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-mScarlet-TEV-Strep-His

Plasmid

#210823

Purpose

Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

Depositor

Luke Chao

Publication

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

mScarlet

Tags

TwinStrep, 10xHis

Expression

Bacterial

Promoter

T7

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pQ-Elo-HB-C-A(1-630)

Plasmid

#171086

Purpose

Co-expresses of ΔC-ELOA containing Elongin complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding all 3Elongin subunits, with a 6-His tag on ELOB

Depositor

Joan Conaway

Publication

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Inserts

Elongin B (ELOB Human)
Elongin C (ELOC Human)
Elongin A (ELOA Human)

Tags

His

Expression

Bacterial

Mutation

Deleted N-term 26aa and C-term 142aa for mutant e…

Promoter

T5 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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