30,719 results

1. pET/LEGH

   Plasmid

   #108226

   Purpose

   Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

   Depositor

   Didier Picard

   Article

   Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

   Insert

   LBT-EGFP-His6
   

   Expression

   Bacterial

   Promoter

   T7, lacO

   Available Since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW313lux-hrpR

   Plasmid

   #61436

   Purpose

   pBWF2620 encoding rbs33-hrpR-ter (pBWF2620: pSB3K3 carrying BBa_F2620 (PTet-rbs34-luxR-ter))

   Depositor

   Baojun Wang, Martin Buck

   Article

   Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

   Insert

   F2620-rbs33-hrpR-ter
   

   Expression

   Bacterial

   Promoter

   plux

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. HS-DN-Plk1

   Plasmid

   #105949

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   Plk1 (PLK1 Human)
   

   Expression

   Bacterial

   Mutation

   Dominant negative K82 R

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-Split-Broccoli-Toehold-Switch

   Plasmid

   #87316

   Purpose

   Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P70a

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGST2-Alix (360-702)

   Plasmid

   #17639

   Depositor

   James Hurley

   Article

   Lee et al Nat Struct Mol Biol. 2007 Mar . 14(3):19

   Insert

   Alix (360-702) (PDCD6IP Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Alix V domain

   Available Since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pET42b-A3A-PBE-ΔUGI

   Plasmid

   #119771

   Purpose

   To express the recombinant APOBEC3A in vitro

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

   Insert

   APOBEC3A-nCas9-NLS
   

   Tags

   6*HIS and NLS

   Expression

   Bacterial

   Mutation

   nCas9 has a mutation D10A in wild type SpCas9

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINGS

   Plasmid

   #41121

   Depositor

   Ray Owens

   Type

   Empty backbone

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   March 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. SIRT5

   Plasmid

   #25487

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   SIRT5 (SIRT5 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. KG#65

   Plasmid

   #110933

   Purpose

   Expresses proteins in cholinergic neurons in the ventral nerve cord of C. elegans

   Depositor

   Kenneth Miller

   Article

   Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

   Inserts

   unc-17 beta promoter
   unc-54 3' control region
   

   Expression

   Bacterial

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT783

    Plasmid

    #68920

    Purpose

    NanoLuc expressed constiutively from PAM2 and RC500 RBS, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Insert

    NanoLuc
    

    Expression

    Bacterial

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS913.CB18.31

    Plasmid

    #69569

    Purpose

    TCR beta chain genomic transgenic construct for creating OT-1 mice

    Depositor

    Francis R. Carbone

    Article

    Carbone et al Int Immunol. 1992 Aug;4(8):861-7.

    Insert

    TCR beta (Tcrb Mouse)
    

    Use

    Mouse Targeting

    Expression

    Bacterial

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR2.1 mNFATc2

    Plasmid

    #17858

    Depositor

    Jerry Crabtree

    Article

    Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

    Insert

    NFATc2 (Nfatc2 Mouse)
    

    Expression

    Bacterial

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

13. hsp70:Stathmin1-mKate2

    Plasmid

    #105969

    Purpose

    heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Stathmin1 (STMN1 Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTac-C-luci

    Plasmid

    #65935

    Purpose

    Theophylline Riboswitch C with luciferase gene

    Depositor

    Justin Gallivan

    Article

    Mishler et al Nucleic Acids Res. 2014 Jun;42(10):6753-

    Insert

    luciferase
    

    Expression

    Bacterial

    Available Since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pET28b-NpR5600

    Plasmid

    #45741

    Depositor

    Emily Balskus, Christopher T Walsh

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    NpR5600 (Npun_R5600 Nostoc punctiforme)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCRISP_IS

    Plasmid

    #120425

    Purpose

    This plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

    Depositor

    Tamás Fehér

    Article

    Nyerges et al Synth Biol (Oxf). 2019;4(1):ysz008. doi:

    Insert

    CRISPR spacers targeting IS1, IS5, IS3
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    constitutive

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-TSV (linearized in VP2)

    Plasmid

    #70032

    Purpose

    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region

    Depositor

    Mariet Feltkamp

    Article

    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

    Insert

    TSV full genome, linearized in VP2 gene
    

    Use

    Cloning vector

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJUMP43-2A(sfGFP)

    Plasmid

    #126987

    Purpose

    Level 2 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPS0384

    Plasmid

    #133234

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Available Since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLC-239

    Plasmid

    #73193

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (yhaV) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-yhaV (yhaV )
    

    Expression

    Bacterial

    Promoter

    PrhaB

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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