33,476 results
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Plasmid#180604PurposeExpression of ShCAST under control of Plac with pRO1600 origin.DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
UseSynthetic BiologyExpressionBacterialPromoterpLacAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZE-P9-egfp
Plasmid#201790Purposethe promoter can be recognized by PadRDepositorInsertP9
ExpressionBacterialMutationWTAvailable sinceJuly 11, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
His-DNPH1
Plasmid#203224PurposeFor protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, G38R)-Strep
Plasmid#196209PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationG38RAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIS-MDH1-HIS
Plasmid#184558PurposeBacterial expression of human MDH1 with HIS TagDepositorAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Az
Plasmid#197101PurposeTo express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanineDepositorInsertTyrRS variant, amber suppressor tRNA, kanamycin resistance gene
ExpressionBacterialMutationThr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…Available sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pND004
Plasmid#200950PurposeMBP-tagged E. coli protein expression destination vector for Golden Gate cloningDepositorTypeEmpty backboneTagsMBPExpressionBacterialPromoterT7Available sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23100-B0034m-E0040m-B0015)
Plasmid#197659PurposeExpresses GFP under the control of J23100 and B0034mDepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG1
Plasmid#193799PurposeProtein production of ChemoG1 in bacteriaDepositorInsertChemoG1
Tags10xHisExpressionBacterialPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG2
Plasmid#193800PurposeProtein production of ChemoG2 in bacteriaDepositorInsertChemoG2
Tags10xHisExpressionBacterialMutationEGFP: A206KPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG3
Plasmid#193801PurposeProtein production of ChemoG3 in bacteriaDepositorInsertChemoG3
Tags10xHisExpressionBacterialMutationEGFP:A206K; HT7: L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG 3.2
Plasmid#193803PurposeProtein production of ChemoG3.2 in bacteriaDepositorInsertChemoG 3.2
Tags10xHisExpressionBacterialMutationEGFP:A206K, T225R; HT7: L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG4
Plasmid#193804PurposeProtein production of ChemoG4 in bacteriaDepositorInsertChemoG4
Tags10xHisExpressionBacterialMutationEGFP:A206K; HT7: E143R, E147R, L271EPromoterT7Available sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-Strep
Plasmid#180024PurposeCodon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.DepositorInsertALIX(1-868, P801G)-Strep (PDCD6IP Human)
TagsStrep tag (WSHPQFEK)ExpressionBacterialMutationP801GAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-ALIX(P801G)
Plasmid#180025PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.DepositorInsertGB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)
TagsGB1-His6-TEVExpressionBacterialMutationP801GAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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hPU.1ΔN165-pET28b
Plasmid#199796PurposeExpressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coliDepositorAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO_MT3
Plasmid#200303PurposeBacterial expression plasmids for production of recombinant metallothionein 3 containing N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubilityDepositorAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
TagsHis6-SUMO and designed intein CExpressionBacterialPromoterT7Available sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPO685
Plasmid#195300PurposepBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.DepositorInsertMcCAST Cascade and native array with PaqCI entry site
ExpressionBacterialPromoterArabinose inducible arapBADAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only