29,937 results

1. pACYCDuet_D27NDHFR_WTTS

   Plasmid

   #91244

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D27N

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACYCDuet_F31YG121VDHFR_WTTS

   Plasmid

   #91229

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F31YG121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACYCDuet_G121VDHFR_WTTS

   Plasmid

   #91233

   Purpose

   bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

   Depositor

   Kimberly Reynolds

   Article

   Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

   Inserts

   folA
   thyA
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   G121V

   Promoter

   T7

   Available since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FGFR3_HUMAN_D0

   Plasmid

   #79731

   Purpose

   This plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Nicholas Levinson, Markus Seeliger, John Chodera

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   FGFR3 (FGFR3 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pTMS1

   Plasmid

   #87799

   Purpose

   For constructing MBP-SUMO-fusion proteins with N-terminal His tag and C-terminal strep II tag using "blunt-end" cloning.

   Depositor

   Yu-Zhu Zhang

   Article

   Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

   Type

   Empty backbone

   Use

   Tags

   6xHis and Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-T Dmel Or69a

   Plasmid

   #59674

   Purpose

   Probe for Drosophila melanogaster Or69a expression

   Depositor

   Richard Axel, Leslie Vosshall

   Article

   Vosshall et al Cell. 1999 Mar 5;96(5):725-36.

   Insert

   Or69a (Or69a Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pSopB2xHA

   Plasmid

   #112513

   Purpose

   PsopB driven sopB-2xHA and sigE

   Depositor

   Olivia Steele-Mortimer

   Article

   Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

   Insert

   sopB-2xHA/sigE operon
   

   Use

   Tags

   2xHA

   Expression

   Bacterial

   Mutation

   SopB C-terminal 2xHA

   Promoter

   PsopB

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH6-mNeon

   Plasmid

   #178460

   Purpose

   The mNeonGreen fluorescent protein with an N-terminal 6xHis-tag in pET28a.

   Depositor

   Siegfried Musser

   Article

   Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

   Insert

   mNeonGreen
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK357

   Plasmid

   #71568

   Purpose

   Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)

   Depositor

   T. J. Kappock

   Article

   Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

   Insert

   succinyl-CoA:acetate CoA-transferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDIV494

    Plasmid

    #177701

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX SHIP1-SH2

    Plasmid

    #46492

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHIP1 (INPP5D Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-118

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAW209

    Plasmid

    #113188

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21a - Mtb-TF

    Plasmid

    #111513

    Purpose

    Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    Trigger Factor
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBbA7c-RFP

    Plasmid

    #35317

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-HA-CPDSalI

    Plasmid

    #38254

    Depositor

    Matthew Bogyo, Aimee Shen

    Article

    Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

    Type

    Empty backbone

    Use

    Tags

    6xHis, HA, and Vibrio cholerae MARTX toxin cystei…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC247

    Plasmid

    #65890

    Purpose

    Strong P2 activator plasmid for two-strain system

    Depositor

    Matthew Bennett

    Article

    Chen et al Science. 2015 Aug 28;349(6251):986-9. do

    Insert

    RhlI (rhlI )
    

    Use

    Tags

    LAA tagged

    Expression

    Bacterial

    Mutation

    Promoter

    Prhl/lac-s

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b(+)-SPN+IFS

    Plasmid

    #83372

    Purpose

    Expresses SPN and IFS complex in E. coli cell

    Depositor

    SeWon Suh

    Article

    Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

    Inserts

    SPN
    IFS
    

    Use

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB3aZ_14*

    Plasmid

    #98542

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Brigitte Gasser, Michael Sauer, Diethard Mattanovich

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p15aC-4D1D

    Plasmid

    #107864

    Purpose

    Bacterial expression of PIS and PI4K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-kinase β
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWT050a

    Plasmid

    #107899

    Purpose

    W2.4-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA4 and is part of CAMERA 2.4

    Depositor

    David Liu

    Article

    Tang et al Science. 2018 Feb 15. pii: science.aap89

    Insert

    PLacO-sgRNA1, PRha-sgRNA4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge