32,417 results
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Plasmid#122573PurposeMAGIC donor plasmid (Himar transposon with catP/sfGFP payload)DepositorInsertcatP/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30_Halotag_K73T_v2
Plasmid#183692PurposeBacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)DepositorInsertER Halo(K73T)
ExpressionBacterialMutationK73T in HalotagAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSV2_P0026-mCherryBb
Plasmid#118239PurposeKanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0026 promoter driven mCherryDepositorInsertbb0026 promoter driven mCherry
ExpressionBacterialAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
NSSCP
Plasmid#183190PurposemRFP-PopZ expressed under J23116 promoter; split N terminal of TEV protease-SpmXΔC expressed under Tac promoter; SpmXΔC-split C terminal of TEV protease expressed under Tac promoter; DivIVA-sfGFP-TEVDepositorInsertsmRFP-PopZ
DivIVA-GFP(tevR-50)
SpmX-TEV(C)
TEV(N)-SpmX
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rb-379-928-pGex2TK
Plasmid#21749DepositorInsertRb (RB1 Human)
TagsGSTExpressionBacterialMutationdeletion mutant ORF contains aa 379-928 of wt Rb …Available SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pD434-SR rat Alpha-synuclein
Plasmid#89076Purposebacterial expression of rat Alpha-synucleinDepositorAvailable SinceMarch 17, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-29b-3xGluGlu-c-jun-His6
Plasmid#129227PurposeEncodes 3xGluGlu tagged human c-Jun for purification by nickel affinityDepositorAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBDK
Plasmid#135190PurposeIntegration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selectionDepositorInsertKanR
UseSynthetic BiologyExpressionBacterialPromotercatAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOPO mCherry
Plasmid#68490PurposeTOPO construct expressing mCherry for generation of GMAP-compatible geneDepositorInsertmCherry
UseGmapExpressionBacterialPromoterPlacAvailable SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRaGE Pyl TAG GFP Y35TAG
Plasmid#160041PurposeEncodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.DepositorInsertsPyrrolysyl tRNA synthetase (Methanosarcina mazei)
Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
deGFP
UseSynthetic BiologyTagsHis-tagExpressionBacterialMutationChanged tyrosine 35 to TAG stop-codon (Site numbe…PromoterP70b promoter and T500 terminator, Vibrio natrieg…Available SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only