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Showing: 1281 - 1300 of 29574 results

  1. p2B-55

    Plasmid
    #177924
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    TDH3
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177924-plasmid-map-sequence-id-349994

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  2. pAJE3-E7

    Plasmid
    #214359
    Purpose
    Expresses the 2nd-generation "E7" Tet2 Methanocaldococcus jannaschii (Mj) aminoacyl tRNA synthetase/tRNA pair for encoding Tet2 noncanonical amino acids into TAG sites of proteins
    Depositor
    Ryan Mehl
    Article
    Eddins et al Bioconjug Chem. 2023 Dec 20;34(12):2243-
    Inserts
    2nd generation Tet2 "E7" aminoacyl tRNA synthetase
    Lpp promoted M. jannaschii tRNA
    Aminoglycoside-3''-adenyltransferase (Spectinomycin/streptomycin Resistance Gene)
    Orthogonal "D4II" ColE1 origin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    AmpR and lpp
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214359-plasmid-map-sequence-id-424910

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  5. pBHSTC104

    Plasmid
    #124356
    Purpose
    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    gp41-1 N-intein and C-intein (bicistronic)
    Use
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Mutation
    Promoter
    STC
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124356-plasmid-map-sequence-id-242355

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  6. SPYtag-Histag-Avidin

    Plasmid
    #214836
    Purpose
    This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    SPYtag-Histag-Avidin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214836-plasmid-map-sequence-id-423115

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  8. mTurquoise2-pBAD

    Plasmid
    #54844
    Purpose
    Localization: Protein Expression Vector , Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    6x His, T7 tag, and TEV site
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54844-plasmid-map-sequence-id-139002

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  9. pET23a-H6-TEV-cSrc

    Plasmid
    #214233
    Purpose
    Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings
    Depositor
    Neel Shah
    Article
    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1
    Insert
    cSrc Kinase domain (SRC Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214233-plasmid-map-sequence-id-422793

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  15. pAIDA1

    Plasmid
    #79180
    Purpose
    A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags
    Depositor
    Gen Larsson
    Article
    Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi
    Insert
    Adhesin Involved in Diffuse Adherence
    Use
    Tags
    His and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    lacUV5
    Available since
    Jan. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    79180-plasmid-map-sequence-id-170854

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  17. Cys-60nmSAH-SPYcatcher

    Plasmid
    #214840
    Purpose
    This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al bioRxiv 2024.01.21.576499.
    Insert
    Cys-60nmSAH-SPYcatcher
    Use
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214840-plasmid-map-sequence-id-425312

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  19. pTOX8

    Plasmid
    #127453
    Purpose
    Encodes MqsR toxin and tsPurple chromoprotein. CAM-R
    Depositor
    Matthew Waldor
    Article
    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii
    Inserts
    mqsR toxin
    tsPurple chromoprotein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Codon optimized from protein sequence in FPbase
    Promoter
    apFAB46 and pRha
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127453-plasmid-map-sequence-id-251148

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  20. p0857 pBR322 SV40

    Plasmid
    #10877
    Depositor
    Peter Howley
    Insert
    SV40
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 26, 2005
    Availability
    Academic Institutions and Nonprofits only
Showing: 1281 - 1300 of 29574 results