30,535 results

1. pLPO1

   Plasmid

   #209975

   Purpose

   Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   replication origin (256_rep)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSRKBB-empty

   Plasmid

   #59449

   Purpose

   Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.

   Depositor

   Claudia Schmidt-dannert

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   modified lac

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUCP20T-tdTomato

   Plasmid

   #78469

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   tdTomato
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA707

   Plasmid

   #186238

   Purpose

   KanR-BBR1 entry vector for HR donor

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSL-MS2-12X

   Plasmid

   #27119

   Depositor

   Robert Singer

   Article

   Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

   Insert

   MS2 binding sites (12)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-Himar-dCas9

   Plasmid

   #137079

   Purpose

   Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSJX022

   Plasmid

   #232325

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and cat

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119 and Pvan

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSJX016

   Plasmid

   #232324

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119 and Pvan

   Available Since

   June 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-21a_3xNLS_SpCas9_protein_expression

   Plasmid

   #114365

   Purpose

   Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

   Insert

   3xNLS_SpCas9
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHEN-CoV2-nanobody-G6

    Plasmid

    #194589

    Purpose

    Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

    Depositor

    Gerald McInerney

    Article

    Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

    Insert

    G6 (S )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. p11d-tRPA(123)

    Plasmid

    #102613

    Purpose

    Expression of human RPA in E. coli

    Depositor

    Marc Wold

    Article

    Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

    Inserts

    RPA1 (RPA1 Human)
    RPA2 (RPA2 Human)
    RPA3 (RPA3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC18T-mini-Tn7T-Gm-eyfp

    Plasmid

    #65031

    Purpose

    GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    eyfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB150

    Plasmid

    #101661

    Purpose

    Opto-T7RNAP*(563)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD33-Gm

    Plasmid

    #65098

    Purpose

    bacterial expression vector with Gentamycin selection, based on pBAD33

    Depositor

    Juan Tomas

    Article

    Jimenez et al J Bacteriol. 2009 Apr;191(7):2228-36. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTA-Mob 2.0

    Plasmid

    #149662

    Purpose

    Conjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.

    Depositor

    Bogumil Karas

    Article

    Soltysiak et al Int J Mol Sci. 2019 Oct 21;20(20). pii:

    Type

    Empty backbone

    Use

    Synthetic Biology; Conjugation helper plasmid

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET His6 LIC cloning vector (2Bc-T)

    Plasmid

    #37236

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBbS5a-Opto-Cre-Vvd-2

    Plasmid

    #160400

    Purpose

    Updated light-inducible Cre recombinase split with Vivid photodimers for bacterial expression.

    Depositor

    Mary Dunlop

    Article

    Sheets et al ACS Synth Biol. 2020 Feb 21;9(2):227-235

    Insert

    Opto-Cre-Vvd-2
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacUV5

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOxyR_rfp

    Plasmid

    #194155

    Purpose

    Red Fluorescent Reporter of intracellular hydrogen peroxide

    Depositor

    Sven Panke

    Article

    Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

    Insert

    transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PoxyR, PoxyS

    Available Since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6xHis-MBP-huBsCas12a

    Plasmid

    #174671

    Purpose

    Expresses 6xHis-MBP tagged BsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTCF5 HLA-A*01:01

    Plasmid

    #180448

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*01:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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