30,410 results
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Plasmid#53734PurposeExpression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertalpha NTD-Beta flap fusion
UseTagsExpressionBacterialMutationPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA
Plasmid#225079PurposeExpresses precursor peptide ChrA in E.coliDepositorInsertChrA
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNCS mTangerine
Plasmid#91763PurposeBacterial expression of a fluorescent protein, monomeric Tangerine (mTangerine)DepositorInsertmonomeric Tangerine
UseTagsno tagExpressionBacterialMutationPromoterT7Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KO (KanR, 186)
Plasmid#45985DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
LbCpf1-2NLS
Plasmid#102566Purposebacterial expression of LbCpf1-2NLSDepositorInsertLbCpf1
UseTags6xHis-MBP-TEV and HA-2xSV40NLSExpressionBacterialMutationPromoterT7Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS mHoneydew
Plasmid#91760PurposeBacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)DepositorInsertmonomeric Honeydew
UseTagsno tagsExpressionBacterialMutationPromoterT7Available SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFC902
Plasmid#106904PurposeThe vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1DepositorInsertscas9
pyrG
U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationPromoterAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FAM98A-Halo
Plasmid#175329PurposeProduction of HaloTag fusion proteinDepositorInsertFAM98A (FAM98A Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBB540
Plasmid#27393DepositorInsertgrpE, clpB
UseTagsExpressionBacterialMutationPromoterAvailable SinceApril 26, 2012AvailabilityAcademic Institutions and Nonprofits only