32,115 results
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Plasmid#160735PurposeSingle-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA and has BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFE-Reu-FI-prk
Plasmid#162696PurposeExpresses R. eutropha Form IC rubisco and S. elongatus PrkDepositorInsertR. eutropha CbbLS and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-XPLN
Plasmid#73375PurposeGST-tagged expression of XPLN (ARHGEF3) in bacteriaDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCV019-catP
Plasmid#232825PurposeRandom transposon mutagenesis vector for Bacteroidales species - chloramphenicolDepositorInsertcatP
ExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFC334
Plasmid#87846PurposeAMA1 plasmid with Aspergillus optimized Cas9, argB selection marker and yA specific sgRNA expressed with ribozymesDepositorInsertsCas9
argB
yA specific sgRNA
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterA. nidulans gpdA promoter with Hammerhead ribozym…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pet16b ALIX Bro1 (1-359)
Plasmid#80641PurposeResidues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428DepositorInsertALIX Bro1, residues 1-359 (PDCD6IP Human)
TagsHis TagExpressionBacterialMutationResidues 1-359PromoterT7Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET32-sLatherin
Plasmid#61685Purposebacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusionDepositorInsertLatherin (LATH horse)
TagsHIS and Trx-Tag thioredoxin proteinExpressionBacterialMutationCodon optimized for E.coliPromoterT7Available SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSRK2706
Plasmid#78571PurposeExpresses untagged human rhinovirus 3C protease in E. coliDepositorAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLG008 (Ec78 retron)
Plasmid#157886PurposeExpresses the Ec78 retron defense systemDepositorInsertEc78 retron
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chPylRS(IPYE)
Plasmid#99222PurposeExpression plasmid encoding chPylRS(IPYE) and pylTDepositorInsertPlpp chPyl(IPYE)RS; PproK pylT
ExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable SinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-KM
Plasmid#203587PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFC333
Plasmid#87844PurposeAMA1 plasmid with Aspergillus optimized Cas9 and ble selection markerDepositorInsertsCas9
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mCherry
Plasmid#102629PurposeIn vitro expression of mCherry from the T7 promoterDepositorInsertmCherry
ExpressionBacterialPromoterT7Available SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Ag43
Plasmid#107743PurposeExpresses Ag43 under the control of an L-arabinose transcriptional regulatorDepositorInsertAg43 (flu )
ExpressionBacterialMutationremoved all pstI cut sites with silent mutations,…PromoterpBADAvailable SinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mKate2
Plasmid#133452PurposeExpresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mKate2
TagsGSGSGS linker and His6ExpressionBacterialPromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBADmod1-linker2-gamS
Plasmid#45833DepositorInsertgamS
UseSynthetic BiologyTagsHistagExpressionBacterialPromoterpBADAvailable SinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBbE8k-RFP
Plasmid#35270DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterBADAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDEST-GST-FBW7 alpha
Plasmid#197456PurposeThe plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.DepositorInsertFBW7 (FBXW7 Human)
UseIn vitro translationTagsGSTExpressionBacterialMutationSee depositor comments belowPromoterT7Available SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRAM18dSGK[MCS]
Plasmid#84684PurposeCan be used for the transformation of rickettsiaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-luciferase
Plasmid#190721Purposefine tune expression of luciferase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-luciferase
UseLuciferaseExpressionBacterialAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrcHIS-wt7D12
Plasmid#125268PurposeExpresses wild type 7D12 anti-EGFR nanobodiesDepositorInsert7D12 nanobody
TagsHis-TagExpressionBacterialPromoterTrcAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+) pCopA RBS30 sfgfp
Plasmid#226371PurposePlasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30DepositorInsertsfGFP
Tags6x HisTagExpressionBacterialPromotercopA promoter (pCopA) and RBS30 regulationAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIS003
Plasmid#194014PurposeThis plasmid is designed for use in a procedure to synthesize single-stranded DNA containing only the insert.DepositorInsertC16 Ex4 XTR f1
ExpressionBacterialAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
KEAP1 (KEAP1A-c029)
Plasmid#110247PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFE-Rs-FI-prk
Plasmid#162697PurposeExpresses R. spaeroides Form IC rubisco and S. elongatus PrkDepositorInsertR. spaeroides Form IC rubisco and S. elongatus Prk
TagsN terminal 6x His tag on PRKExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29-neoLux1.2
Plasmid#229952PurposeBacterial expression for de novo luciferase, neoLux1.2DepositorInsertneoLux1.2
TagsHis-tagExpressionBacterialPromoterT7Available SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
TagsHisExpressionBacterialAvailable SinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc
Plasmid#117728PurposeIntegration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc geneDepositorInsertNanoLuc
UseLuciferaseExpressionBacterialAvailable SinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-Ad5_pvc10-HUHe
Plasmid#243745PurposePVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with HUHe on CTDDepositorInsertpvc13-Ad5_pvc10-HUHe
ExpressionBacterialAvailable SinceSept. 24, 2025AvailabilityAcademic Institutions and Nonprofits only