30,260 results

1. pEvol-pAzFRS.1.t1

   Plasmid

   #73547

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

   Depositor

   Farren Isaacs

   Article

   Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

   Inserts

   pAzFRS.1.t1
   pAzFRS.1.t1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJYS1Peftu

   Plasmid

   #85546

   Purpose

   Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   recT
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   etfu and unknown

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJYS3_ΔcrtYf

   Plasmid

   #85542

   Purpose

   Constitutive transcription of FnCpf1 and crRNA of crtYf

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Inserts

   Cpf1
   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207374

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N

   Promoter

   T7

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFCiKm7

   Plasmid

   #167521

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinRAM-KmR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPP085

   Plasmid

   #158795

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-32a(+)-TGFB1m

   Plasmid

   #120286

   Purpose

   Produce human recombinant mTGFB1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   monomeric Transforming Growth Factor-β1
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promotor

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPJ23116-MS2-rep

   Plasmid

   #184842

   Purpose

   Expression of the rep protein of phage MS2

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Replicase gene MS2 phage
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23116

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMevT

   Plasmid

   #17815

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFPC1-muscle Amphiphysin 2

    Plasmid

    #22213

    Depositor

    Pietro De Camilli

    Article

    Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

    Insert

    Amphiphysin II (BIN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

    Plasmid

    #117728

    Purpose

    Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCP20T-eyfp

    Plasmid

    #78466

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    eYFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDonor14-N7HELIX-KanR

    Plasmid

    #190663

    Purpose

    Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pATetO 6XHis T4 DNA Ligase

    Plasmid

    #206207

    Purpose

    Inducible expression of T4 DNA ligase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    T4 DNA ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRMCD28

    Plasmid

    #167556

    Purpose

    Plasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mapping

    Depositor

    Renato Morona

    Article

    Daniels et al Mol Microbiol. 1998 Jun;28(6):1211-22. d

    Type

    Empty backbone

    Use

    Tags

    PhoA'

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKL10-mRuby-iATPSnFR1.0 bb118

    Plasmid

    #158980

    Purpose

    Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mruby-iATPSNfR1.0
    

    Use

    Tags

    mruby3

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#102557

    Promoter

    Bba_J23118 (sigma 70)

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSC101_TIMER

    Plasmid

    #103057

    Purpose

    low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

    Depositor

    Dirk Bumann

    Article

    Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

    Insert

    TIMER-bac
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations: S4T and S179T

    Promoter

    constitutive ybaJp promoter

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbA5a-sufCDSUB

    Plasmid

    #195055

    Purpose

    Expresses SUF operon from B. subtilis from IPTG-inducible promoter

    Depositor

    Greg Bokinsky

    Article

    D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

    Insert

    sufCDSUB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAKgfplux3

    Plasmid

    #199304

    Purpose

    Fluorescence and bioluminescence expression in Gram-negative bacteria

    Depositor

    Attila Karsi

    Article

    Ozdemir et al Pathogens. 2023 May 2;12(5):670. doi: 10

    Insert

    Chloramphenicol acetyltransferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge