30,416 results

1. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

   Plasmid

   #199605

   Purpose

   Expression of cell permeable opAsCas12a in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   opCas12a-T8N
   

   Use

   CRISPR

   Tags

   6xHis, Twin-strep, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-6xHis-(pos36)GFP

   Plasmid

   #62937

   Purpose

   Expression of 6xHis-(+36)GFP in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (pos36)GFP
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET51b(+)_SNAP-tag2

   Plasmid

   #226512

   Purpose

   Bacterial cell expression of SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   SNAP-tag2
   

   Use

   Tags

   His-tag (10x) and Strep-Tag II,

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-MHL

   Plasmid

   #26096

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Cheryl Arrowsmith

   Type

   Empty backbone

   Use

   Tags

   6xHis and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 20, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCDF-Frb-v1

   Plasmid

   #201923

   Purpose

   E. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.

   Depositor

   Ryan Mehl, Richard Cooley

   Article

   Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

   Inserts

   FrbA
   FrbB
   FrbC
   FrbD
   FrbE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (modified), T7 (modified, moderate), T7 (modif…

   Available Since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPbcas13b

   Plasmid

   #89906

   Purpose

   Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-mTagBFP2

   Plasmid

   #34632

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I174A compared to mTagBFP (enhanced photostabilit…

   Promoter

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pE-SUMO CRYAA

   Plasmid

   #80756

   Purpose

   Expression of human alpha A-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYAA (CRYAA Human)
   

   Use

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFREE

   Plasmid

   #92050

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRhamBAD and ptet

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST17-UnaG

    Plasmid

    #203485

    Purpose

    For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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